Soybean cultivar selection for Bioregenerative Life Support Systems (BLSSs) – Hydroponic cultivation

Four soybean cultivars (‘Atlantic’, ‘Cresir’, ‘Pr91m10’ and ‘Regir’), selected through a theoretical procedure as suitable for cultivation in BLSS, were evaluated in terms of growth and production. Germination percentage and Mean Germination Time (MGT) were measured. Plants were cultivated in a growth chamber equipped with a recirculating hydroponic system (Nutrient Film Technique). Cultivation was performed under controlled environmental conditions (12 h photoperiod, light intensity 350 μmol m⁻² s⁻¹, temperature regime 26/20 °C light/dark, relative humidity 65–75%). Fertigation was performed with a standard Hoagland solution, modified for soybean specific requirements, and EC and pH were kept at 2.0 dS m⁻¹ and 5.5 respectively.     

Plasma effects of active ion beam injections in the ionosphere at rocket altitudes

Data from ARCS rocket ion beam injection experiments will be primarily discussed in this paper. There are three results from this series of active experiments that are of particular interest in space plasma physics. These are the transverse acceleration of ambient ions in the large beam volume, the scattering of beam ions near the release payload, and the possible acceleration of electrons very close to the plasma generator which produce intense high frequency waves. The ability of 100 ma ion beam injections into the upper E and F regions of the ionosphere to produce these phenomena appear to be related solely to the process by which the plasma release payload and the ion beam are neutralized. Since the electrons in the plasma release do not convect with the plasma ions, the neutralization of both the payload and beam must be accomplished by large field-aligned currents (milliamperes/square meter) which are very unstable to wave growth of various modes. Future work will concentrate on the wave production and wave-particle interactions that produce the plasma/energetic particle effects discussed in this paper and which have direct application to natural phenomena in the upper ionosphere and magnetosphere.     

Energetic ion emission for active spacecraft control

Future space missions aiming at the accurate measurement of cold plasmas and DC to very low frequency electric fields will require that the potential of their conductive surfaces be actively controlled to be near the ambient plasma potential. In the near-Earth space these spacecraft are usually solar-cell powered; consequently, parts of their surface are most of the time exposed to solar photons. Outside the plasmasphere, a positive surface potential due the dominance of surface-emitted photoelectrons over ambient plasma electrons is to be expected. Photo- and ambient electrons largely determine the potential and positive values between a few Volts up to 100 V have been observed. Active ion emission is the obvious solution of this problem. A liquid metal ion emitter and a saddle field ion emitter are nearing the stage of flight unit fabrication. We will attempt to clamp the spacecraft potential to values close to the plasma potential. We present first results from vacuum chamber tests and describe the emission behaviour and characteristics of emitters producing, respectively, In⁺ and N₂⁺ beams with an energy of ≥ 5 keV.     

Occurrence of equatorial plasma bubbles over Kolhapur

This paper reports the nightglow observations of OI 630.0 nm emissions, made by using all sky imager operating at low latitude station Kolhapur (16.8°N, 74.2°E and dip lat. 10.6°N) during high sunspot number years of 24th solar cycle. The images are analyzed to study the nocturnal, seasonal and solar activity dependence occurrence of plasma bubbles. We observed EPBs in images regularly during a limited period 19:30 to 02:30 LT and reach maximum probability of occurrence at 22:30 LT. The observation pattern of EPBs shows nearly no occurrence during the month of May and it maximizes during the period October–April. The equinox and solstice seasonal variations in the occurrence of plasma bubbles show nearly equal and large differences, respectively, between years of 2010–11 and 2011–12.     

High resolution time profile of decimetric type-III bursts

Type-III bursts are signatures of the electron beams accelerated during the solar flares, their observation and investigation provide information of the acceleration processes, the characteristics of the exciting agent and the acceleration site. The Brazilian Solar Spectroscope (BSS), in operation at INPE, Brazil, have recorded type-III radio bursts in decimetric range (2050–2250 MHz) with high time resolution of 20 ms. Decimetric reverse drift bursts are possibly generated in a dense loop by electron beams travelling towards the photosphere. Hence their time profiles should carry signatures of the density inhomogenities in the loop. Here the temporal and spectral characteristics of decimetric type-III bursts are presented.     

Escaping near-relativistic electron beams from the solar corona

Processes in the solar corona are prodigious accelerators of energetic ions, and electrons. The angular distribution, composition, and spectra of energetic particles observed near Earth gives information on the acceleration mechanisms. A class of energetic particle observations particularly useful in understanding the solar acceleration is the near-relativistic impulsive beam-like electron events. During five years of operation the Advanced Composition Explorer (ACE) has measured well over 400 electron events. Approximately 25% of these electron events are impulsive beam-like events that are released onto interplanetary field lines predominantly from western solar longitudes. We extend our initial 3 year study during the rise to solar maximum (Haggerty and Roelof, 2002; Simnett et al., 2002) to a five year statistical analysis of these beam-like energetic electron events in relationship to optical flares, microwave emission, soft X-ray emission, metric and decametric type-III radio bursts, and coronal mass ejections.     

Comment on estimating the solar proton environment that may affect Mars missions.

Estimates of the energetic proton environment for a Mars mission are generally extrapolated from the solar proton observations at 1 AU. We find that solar particle events may be divided into two general classes. Events dominated by a near-sun injection of particles onto interplanetary magnetic field lines leading to the spacecraft position represent the "classical" solar particle event associated with solar activity. This class of event will scale in radial distance by the classical power law extrapolation. The extended-interplanetary-shock source generates a maximum flux as the shock passes the detection location. This class of event typically generates maximum fluence, but in this case, the flux and fluence will not scale in the classical manner with radial distance.     

Induced abnormality in Mir- and Earth grown Super Dwarf wheat.

Super-dwarf wheat grown on the Mir space station using the Svet "Greenhouse" exhibited morphological, metabolic and reproductive abnormalities compared with Earth-grown wheat. Of prominent importance were the abnormalities associated with reproductive ontogeny and the total absence of seed formation on Mir. Changes in the apical meristem associated with transition from the vegetative phase to floral initiation and development of the reproductive spike were all typical of 'Super-Dwarf' wheat up to the point of anthesis. Observation of ruptured anthers from the Mir-grown plants revealed what appeared to be normally developed pollen. These pollen gains, however, contained only one nuclei, while normal viable pollen is tri-nucleate. A potentially important difference in the flight experiment, compared with ground reference studies, was the presence of a high level of atmospheric ethylene (1,200 ppb). Ground studies conducted by exposing 'Super-Dwarf' wheat to ethylene just prior to anthesis resulted in manifestation of the same abnormalities observed in the space flight samples.     

Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.