Viability of barley seeds after long-term exposure to outer side of international space station

Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.     

Cryptographic Digital Communication

This paper reviews the fundamentals of cryptographic digital systems and defines and discusses the two basic types of ciphers. The remainder of this article examines the word and bit error rates in digital al communication systems with block or stream ciphers. Upper bounds and ensemble averages of the error rates are obtained for both ciphers. The effect of modulation type on the degradation in communication system performance caused by the addition of cryptography is analyzed. A comparison is made between block and stream ciphers with respect to their effects on degradation.     

Analysis of a spacecraft life support system for a Mars mission

This report summarizes a trade study conducted as part of the Fall 2002 semester Spacecraft Life Support System Design course (ASEN 5116) in the Aerospace Engineering Sciences Department at the University of Colorado. It presents an analysis of current life support system technologies and a preliminary design of an integrated system for supporting humans during transit to and on the surface of the planet Mars. This effort was based on the NASA Design Reference Mission (DRM) for the human exploration of Mars [NASA Design Reference Mission (DRM) for Mars, Addendum 3.0, from the world wide web: http://exploration.jsc.nasa.gov/marsref/contents.html.]. The integrated design was broken into four subsystems: Water Management, Atmosphere Management, Waste Processing, and Food Supply. The process started with the derivation of top-level requirements from the DRM. Additional system and subsystem level assumptions were added where clarification was needed. Candidate technologies were identified and characterized based on performance factors. Trade studies were then conducted for each subsystem. The resulting technologies were integrated into an overall design solution using mass flow relationships. The system level trade study yielded two different configurations--one for the transit to Mars and another for the surface habitat, which included in situ resource utilization. Equivalent System Mass analyses were used to compare each design against an open-loop (non-regenerable) baseline system.     

The Tunguska event in 1908: evidence from tree-ring anatomy

We analyzed tree rings in wood samples collected from some of the few surviving trees found close to the epicenter (within 4-5 km) of the Tunguska event that occurred on the last day of June 1908. Tree-ring growth shows a depression starting in the year after the event and continuing during a 4-5-year period. The most remarkable traces of the event were found in the rings' anatomical structure: (1) formation of "light" rings and a reduction of maximum density in 1908; (2) non-thickened tracheids (the cells that make up most of the wood volume) in the transition and latewood zones (the middle and last-formed parts of the ring, respectively); and (3) deformed tracheids, which are located on the 1908 annual ring outer boundary. In the majority of samples, normal earlywood and latewood tracheids were formed in all annual rings after 1908. The observed anomalies in wood anatomy suggest two main impacts of the Tunguska event on surviving trees--(1) defoliation and (2) direct mechanical stress on active xylem tissue. The mechanical stress needed to fell trees is less than the stress needed to cause the deformation of differentiating tracheids observed in trees close to the epicenter. In order to resolve this apparent contradiction, work is suggested on possible topographic modification of the overpressure experienced by these trees, as is an experimental test of the effects of such stresses on precisely analogous growing trees.     

Bumpy ride back to space

Plans for U.S. return to the International Space Station via STS-114 are described with emphasis on safety of flight hardware and safety of the space station and the space shuttle.     

Geysers spawn hot new nanotechnology

The article reports on efforts by NASA scientists to build nanostructures from thermophilic cyanobacteria living in hot springs. Heat shock protein 60 (HSP60) is purified and engineered to serve as a substrate for quantum dots that are about 1-10 nm across, much smaller than the typical 130 nm found in standard computer chips.     

The space-developed dynamic vertical cutoff rigidity model and its applicability to aircraft radiation dose.

We have developed a dynamic geomagnetic vertical cutoff rigidity model that predicts the energetic charged particle transmission through the magnetosphere. Initially developed for space applications, we demonstrate the applicability of this library of cutoff rigidity models for computing aircraft radiation dose. The world grids of vertical cutoff rigidities were obtained by particle trajectory tracing in a magnetospheric model. This reference set of world grids of vertical cutoff rigidities calculated for satellite altitudes covers all magnetic activity levels from super quiet to extremely disturbed (i.e., Kp indices ranging from 0 to 9+) for every three hours in universal time. We utilize the McIlwain "L" parameter as the basis of the interpolation technique to reduce these initial satellite altitude vertical cutoff rigidities to cutoff rigidity values at aircraft altitudes.     

Physical simulation for low-energy astrobiology environmental scenarios

Speculations about the extent of life of independent origin and the potential for sustaining Earth-based life in subsurface environments on both Europa and Mars are of current and relevant interest. Theoretical modeling based on chemical energetics has demonstrated potential options for viable biochemical metabolism (metabolic pathways) in these types of environments. Also, similar environments on Earth show microbial activity. However, actual physical simulation testing of specific environments is required to confidently determine the interplay of various physical and chemical parameters on the viability of relevant metabolic pathways. This testing is required to determine the potential to sustain life in these environments on a specific scenario by scenario basis. This study examines the justification, design, and fabrication of, as well as the culture selection and screening for, a psychrophilic/halophilic/anaerobic digester. This digester is specifically designed to conform to physical testing needs of research relating to potential extent physical environments on Europa and other planetary bodies in the Solar System. The study is a long-term effort and is currently in an early phase, with only screening-level data at this time. Full study results will likely take an additional 2 years. However, researchers in electromagnetic biosignature and in situ instrument development should be aware of the study at this time, as they are invited to participate in planning for future applications of the digester facility.     

Electrification of Polymer Films in Laboratory and under Space Conditions

A simplified theory of the electrification of polymer films, which permits estimation of the internal electric fields of films irradiated by monoenergetic electrons simulating real streams of plasma electrons during magnetic substorms at a geosynchronous orbit, is suggested. To determine the depth behavior of the forward current and the dosage rate in the irradiated polymer, numerical calculations are made by the Monte Carlo technique. The appearance of electrostatic discharges in thin (5 m) mylar films is shown to be very probable in the orbit's shadow sector under magnetic substorm conditions.