Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

Beagle 2: a proposed exobiology lander for ESA's 2003 Mars Express mission.

The aim of the proposed Beagle 2 small lander for ESA's 2003 Mars Express mission is to search for organic material on and below the surface of Mars and to study the inorganic chemistry and mineralogy of the landing site. The lander will have a total mass of 60kg including entry, descent, and landing system. Experiments will be deployed on the surface using a robotic arm. It will use a mechanical mole and grinder to obtain samples from below the surface, under rocks, and inside rocks. Sample analysis by a mass spectrometer will include isotopic analysis. An optical microscope, an X-ray spectrometer and a Mossbauer spectrometer will conduct in-situ rock studies.     

The Solar Isotope Spectrometer for the Advanced Composition Explorer

The Solar Isotope Spectrometer (SIS), one of nine instruments on the Advanced Composition Explorer (ACE), is designed to provide high- resolution measurements of the isotopic composition of energetic nuclei from He to Zn (Z=2 to 30) over the energy range from ∼10 to ∼100 MeV nucl−1. During large solar events SIS will measure the isotopic abundances of solar energetic particles to determine directly the composition of the solar corona and to study particle acceleration processes. During solar quiet times SIS will measure the isotopes of low-energy cosmic rays from the Galaxy and isotopes of the anomalous cosmic-ray component, which originates in the nearby interstellar medium. SIS has two telescopes composed of silicon solid-state detectors that provide measurements of the nuclear charge, mass, and kinetic energy of incident nuclei. Within each telescope, particle trajectories are measured with a pair of two-dimensional silicon-strip detectors instrumented with custom, very large-scale integrated (VLSI) electronics to provide both position and energy-loss measurements. SIS was especially designed to achieve excellent mass resolution under the extreme, high flux conditions encountered in large solar particle events. It provides a geometry factor of ∼40 cm2 sr, significantly greater than earlier solar particle isotope spectrometers. A microprocessor controls the instrument operation, sorts events into prioritized buffers on the basis of their charge, range, angle of incidence, and quality of trajectory determination, and formats data for readout by the spacecraft. This paper describes the design and operation of SIS and the scientific objectives that the instrument will address. This revised version was published online in June 2006 with corrections to the Cover Date.     

Direction-finding with sparse rectangular dual-size spatialinvariance array

A novel sparse array geometry embedding two sizes of spatial invariances is presented for use with a new ESPRIT-based (estimation of signal parameters via rotational invariance techniques) algorithm for aperture extension. The half-wavelength invariance yields unambiguous but high-variance direction cosine estimates to disambiguate low-variance but cyclically ambiguous estimates from the larger invariance. With larger invariance at 60 half-wavelengths, resolution threshold for two closely spaced emitters is reduced by 50 dB and estimation error by 100-fold. Array design formulas are also presented     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

Review of primary spaceflight-induced and secondary reloading-induced changes in slow antigravity muscles of rats.

We have examined the light and electron microscopic properties of hindlimb muscles of rats flown in space for 1-2 weeks on Cosmos biosatellite flights 1887 and 2044 and Space Shuttle missions Spacelab-3, Spacelab Life Sciences-1 and Spacelab Life Sciences-2. Tissues were obtained both inflight and postflight permitting definition of primary microgravity-induced changes and secondary reentry and gravity reloading-induced alterations. Spaceflight causes atrophy and expression of fast fiber characteristics in slow antigravity muscles. The stresses of reentry and reloading reveal that atrophic muscles show increased susceptibility to interstitial edema and ischemic-anoxic necrosis as well as muscle fiber tearing with disruption of contractile proteins. These results demonstrate that the effects of spaceflight on skeletal muscle are multifaceted, and major changes occur both inflight and following return to Earth's gravity.     

用CBN刀具高速切削灰铸铁的试验研究

研究了CBN刀具高速切削灰铸铁时的刀具前、后刀面磨损、耐用度以及富氧气氛对切削性能的影响,所用的灰铸铁具有不同的铁素体、珠光体比例,最高切削速度达1＇100 m/min.研究表明Amborite刀具的刀尖磨损略大于BZN6000刀具,在较低速度下加工以铁素体为主的灰铸铁时更容易发生月牙洼磨损;BZN6000刀具以700 m/min的切削速度加工铁素体含量较高的灰铸铁时耐用度较高,Amborite刀具的耐用度随切削速度而升高;在富氧气氛中切削可以减轻刀具磨损,这一效果对BZN6000刀具更为显著.文章中对这些现象的原因进行了讨论.     

Structure of the Program of Short-term Prediction of Powerful Solar Flares

Input data of the system are two-dimensional images and one-dimensional distributions of total and polarized solar emission at 5.2 cm wavelength obtained with SSRT. Together with photoheliograms, magnetograms, Hα-filtergrams and characteristics of active regions received from other sources, they form the initial database. The first stage includes superimposing the images, identifying microwave sources with active regions, assigning NOAA numbers to the sources, and determining for each active region the heliolatitude, extent, and inclination angle of the group's axis to the equator. These data are used to calculate the boundaries of longitude zones for each active region. A next stage involves determining the brightness temperatures of microwave sources less than the polarization distribution, the degree of polarization, and microwave emission flux, as well as calculating the parameters of microwave sources. Each parameter is assigned its own value of the weight factor, and the sum of values is used to draw the conclusion about the flare occurrence probability in each active region and on the Sun in general.      

The effect of a magnetic field on stellar pulsations as a singular perturbation problem

The perturbation problem that describes the effect of a weak magnetic field on stellar adiabatic oscillation is considered. This perturbation problem is singular when the magnetic field does not vanish at the stellar surface, and a regular perturbation scheme fails where the magnetic pressure is comparable to the thermodynamic pressure. The application of the Method of Matched Asymptotic Expansion is used to obtain expressions for the eigenfunctions and the eigenfrequencies.     

Definition of Wingtip Devices Shape and Dimensions for a Subsonic Airliner

The results of flow field numerical simulation on the typical wing-body prototype of the modern DLR-F4 airliner under sub- and transonic compressible air flow are presented. Using the DLR-F4 CAD model, the effect of the wingtip end plate area and of the cant angle of a typical Whitcomb winglet is studied. The dependencies of the model lift-to-drag ratio increment on the flat wingtip end plate relative area and on the cant angle of an airfoil Whitcomb winglet are obtained. The concept of an elliptic winglet with a variable cant angle that similar to the winglet used on Airbus A350 is studied. A technique is developed for solving the multi-parameter design optimization task for the Whitcomb winglet, taking the maximum lift-to-drag ratio of the wing as a criterion for optimization.