Near-Earth radiation model deficiencies as seen on CRRES.

The Space Radiation (SPACERAD) experiments on the Combined Release and Radiation Effects Satellite (CRRES) gathered 14 months of radiation particle data in an 18 degrees inclination orbit between 350 km and 36000 km from July 1990 to October 1991. When compared to the NASA radiation belt models AP8 and AE8, the data show the proton model (AP8) does not take into account a second belt formed after major solar flare/shock injection events, and the electron model (AE8) is misleading, at best, in calculating dose in near-Earth orbits. The second proton belt, although softer in energy than the main proton belt, can produce upsets in proton sensitive chips and would produce significant dose in satellites orbiting in it. The MeV electrons observed on CRRES show a significant particle population above 5 MeV (not in the AE8 model) which must be included in any meaningful dose predictions for satellites operating between L-shells of 1.7 and 3.0 RE.     

Cell biology of plant gravity sensing.

The debate about whether gravity sensing relies upon statoliths (amyloplasts that sediment) has intensified with recent findings of gravitropism in starchless mutants and of claims of hydrostatic gravity sensing. Starch and significant plastid sedimentation are not necessary for reduced sensing in mutant roots, but plastids might function here if there were a specialized receptor for plastid mass e.g. in the ER. Alternatively, components in addition to amyloplasts might provide mass for sensing. The nucleus is dense and its position is regulated, but no direct data exist for its role in sensing. If the weight of the protoplast functioned in sensing, why would there be specific cytological specializations favoring sedimentation rather than cell mass? Gravity has multiple effects on plants in addition to gravitropism. There may be more than one mechanism of gravity sensing.     

Solar-interplanetary circumstances associated with the major events in March and June 1991 and comparison with similar events of previous solar cycles.

The solar activity and geomagnetic storm events of March and June 1991 were associated with the appearance of an enhanced particle flux in the trapped radiation belts as discovered by the CRRES satellite observations and later measured by shuttle radiation detectors. The solar-interplanetary conditions associated with these events appear to be a major sequence of activity near the sun's central meridian generating powerful fast interplanetary shocks resulting in major perturbations to the magnetosphere. The solar-interplanetary events in 1991 are discussed and compared to similar activity in the past such as the events in February 1986, August 1972, July 1961, November 1960, and July 1959.     

Dark matter in the outer solar system.

There are now a large number of small bodies in the outer solar system that are known to be covered with dark material. Attempts to identify that material have been thwarted by the absence of discrete absorption features in the reflection spectra of these planetesimals. An absorption at 2.2 micrometers that appeared to be present in several objects has not been confirmed by new observations. Three absorptions in the spectrum of the unusually red planetesimal 5145 Pholus are well-established, but their identity remains a mystery.     

Analysis of the linear amplifier/analog-digital converter interfacein a digital microwave receiver

An analysis of the relationship between a linear amplifier chain and an analog-to-digital converter (ADC) in a digital microwave receiver, with respect to sensitivity and dynamic range issues, is presented. The effects of gain, third-order intermodulation products and ADC characteristics on the performance of the receiver are illustrated and design criteria for the linear amplifier chain (given a specified ADC) are developed. A computer program is included which calculates theoretical receiver performance based on gain and third-order intermodulation product selections. Experimental results are also presented and compared with theoretical values     

Biological degradation and composition of sweet potato biomass - errata

Many challenges are presented by biological degradation in a bioregenerative Controlled Ecological Life Support System as envisioned by the U.S. National Aeronautics and Space Administration. In studies conducted with biodegradative microorganisms indigenous to sweetpotato fields, it was determined that a particle size of 75 microns and incubation temperature of 30°C were optimal for degradation. The composition of the biomass and characterization of plant nutrient solution indicated the presence of potential energy sources to drive microbial transformations of plant waste. Selected indigenous soil isolates with ligno-cellulolytic or sulfate-reducing ability were utilized in biological studies and demonstrated diversity in their ability to reduce sulfate in solution and to utilize alternative carbon sources: a lignin analog 4-hydroxy, 3-methoxy cinnamic acid, cellulose, arabinose, glucose, sucrose, mannitol, galactose, ascorbic acid.     

Molecular analysis of mutagenesis by high LET radiation.

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.     

The impact of alternative mission models on the future orbital debris environment

In reviewing discussions of future directions for space activity, it becomes obvious that there are a large number of groups formulating a wide diversity of plans for the future use of space. These plan alternatives are being made to account for user needs, technology development constraints, economic constraints, and launch support, and each of the plans will have direct or indirect effects on the orbital debris environment in terms of mass to orbit, deposition of operational debris, and control of accidental breakups. Thus it is important to develop the ability to project future debris states for a range of possible space traffic scenarios. The impact that these possible traffic environments would have on space operations forms the basis for studies of alternative options for the usage of space. In this paper, the effects on the orbital debris environment of a base-line mission model and two alternatives are investigated, using a numerical debris environment simulation code under development at JSC.     

Calculations and observations of solar particle enhancements to the radiation environment at aircraft altitudes.

Solar particle events can give greatly enhanced radiation at aircraft altitudes, but are both difficult to predict and to calculate retrospectively. This enhanced radiation can give significant dose to aircrew and greatly increase the rate of single event effects in avionics. Validation of calculations is required but only very few events have been measured in flight. The CREAM detector on Concorde detected the event of 29 September 1989 and also four periods of enhancement during the events of 19-24 October 1989. Instantaneous rates were enhanced by up to a factor ten compared with quiet-time cosmic rays, while flight-averages were enhanced by up to a factor six. Calculations are described for increases in radiation at aircraft altitudes using solar particle spectra in conjunction with Monte Carlo radiation transport codes. In order to obtain solar particle spectra with sufficient accuracy over the required energy range it is necessary to combine space data with measurements from a wide range of geomagnetically dispersed, ground-level neutron monitors. Such spectra have been obtained for 29 September 1989 and 24 October 1989 and these are used to calculate enhancements that are compared with the data from CREAM on Concorde. The effect of cut-off rigidity suppression by geomagnetic activity is shown to be significant. For the largest event on record on 23 February 1956, there are no space data but there are data from a number of ground-level cosmic-ray detectors. Predictions for all events show very steep dependencies on both latitude and altitude. At high latitude and altitude (17 km) calculated increases with respect to cosmic rays are a factor 70 and 500 respectively for 29 September 1989 and 23 February 1956. The levels of radiation for high latitude, subsonic routes are calculated, using London to Los Angeles as an example, and can exceed 1 mSv, which is significantly higher than for Concorde routes from Europe to New York. The sensitivity of the calculations to spectral fitting, geomagnetic activity and other assumptions demonstrates the requirement for widespread carriage of radiation monitors on aircraft.     

Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart.

Little is known about the effect of microgravity on gene expression, particularly in vivo during embryonic development. Using transgenic zebrafish that express the gfp gene under the influence of a beta-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart. Zebrafish embryos, at the 18-20 somite-stage, were exposed to simulated-microgravity for 24 hours. The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy. Our measurements indicated that simulated-microgravity induced a 23.9% increase in GFP-associated fluorescence in the heart. In contrast, the caudal notochord showed a 17.5% increase and the embryo as a whole showed only an 8.5% increase in GFP-associated fluorescence. This suggests that there are specific effects on the heart causing the more dramatic increase. These studies indicate that microgravity can influence gene expression and demonstrate the usefulness of this in vivo model of 'reporter-gene' expression for studying the effects of microgravity.