First radiobiological results of LDEF-1 experiment A0015 with Arabidopsis seed embryos and Sordaria fungus spores.

This article highlights the first results of investigations on the general vitality and damage endpoints caused by cosmic ionizing radiation in dry, dormant plant seeds of the crucifer plant Arabidopsis thaliana (L.) Heynh. and the ascomycete Sordaria fimicola after 69 month stay in space. Wild-type and mutant gene marker lines were included in Free Flyer Biostack containers and exposed on earth and side tray of the LDEF-1 satellite. The damage in biological endpoints observed in the seeds increased in the side tray sample compared to the earth tray sample. For the ascospores we found different effects depending on the biological endpoints investigated for both expositions.     

Potential vascular damage from radiation in the space environment.

Cultured endothelial cells of blood vessels have a Do of 2 Gy for X-rays. A dose of 0.5 Gy of X-rays has an acute effect on vessel diameter. The vessels may show other acute effects such as change in permeability including a change in the blood brain barrier. Changes occurring from late effects of chronic exposure in vascular architecture include telangiectasia and decrease in vascular density. Changes in the perivascular connective tissue particularly collagen may play a role in these changes. After charged particle exposure of 15 and 30 Gy, radiation changes in the blood brain barrier and vascular changes are noted in the nervous system. These long term changes are recorded by PET, MRI, and CT imaging. Chronic exposure to alpha particles causes vascular damage in compact bone resulting in bone infarcts. Using tandem scanning confocal microscopy in-situ imaging of the capillaries and collagen of the papillary dermis provides a non-invasive method of serial recording of changes in irradiated microvasculature.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

Control of microorganisms in flowing nutrient solutions.

Controlling microorganisms in flowing nutrient solutions involves different techniques when targeting the nutrient solution, hardware surfaces in contact with the solution, or the active root zone. This review presents basic principles and applications of a number of treatment techniques, including disinfection by chemicals, ultrafiltration, ultrasonics, and heat treatment, with emphasis on UV irradiation and ozone treatment. Procedures for control of specific pathogens by nutrient solution conditioning also are reviewed.     

Multitarget detection using Kalman-based average displacementestimator

The anomaly in the displacement estimates obtained from a low-level Kalman-based average displacement estimator is used to detect multiple targets in a forward-looking infrared (FLIR) scene. The displacement estimates originating from the targets could be associated with the detected multiple targets and an updated estimated position of the target could be obtained. This procedure could be repeated for all targets in the scene to obtain multitarget tracking. The behavior of the expected value of the displacement estimates as a function of the number of iterations is investigated. The behavior of the displacement error covariance matrix and the Kalman gain matrix are discussed as functions of the number of iterations     

A modified Gaussian sum approach to estimation of non-Gaussiansignals

A Gaussian sum estimation algorithm has previously been developed to deal with noise processes that are non-Gaussian. Inherent in this algorithm is a serious growing memory problem that causes the number of terms in the Gaussian sum to increase exponentially at each iteration. A modified Gaussian sum estimation algorithm using an adaptive filter is developed that avoids the growing memory problem of the previous algorithm while providing effective state estimation. The adaptive filter is comprised of a fixed set of estimators operating in parallel with each individual estimate possessing its own corresponding weighting term. A simulation example illustrates the new non-Gaussian estimation technique     

Editorial: Show Us the Data

Journal of Reducing Space Mission Cost -     

A new deterministic VXI highway interconnect

Programs based upon the VXIbus modular instrumentation standard are playing a significant role in test systems for both defense and commercial applications. Typical single-chassis VXI systems include an embedded computer-based controller as the system host. Multi-chassis systems generally use a parallel bus or a local area network, such as Ethernet, to interconnect the chassis. An important class of multi-chassis test systems requires high I/O throughput, low latency and a highly deterministic I/O response. This paper describes a new highway interconnect system that includes host computer interface options for VMEbus, EISA and PCI bus. A typical system is configured with a host interface, a fiber-optic highway “loop” and slot-0 controllers in the VXI chassis. The system supports up to 126 VXI chassis, an aggregate I/O throughput of 10 megabytes per second, extremely low latency and a highly deterministic I/O response. Typical applications, including jet aircraft engine testing, missile test systems and high-performance wind tunnel data systems are discussed     

Optimal radar threshold determination in Weibull clutter andGaussian noise

A technique is presented for determining the ideal detection threshold when Gaussian noise and Weibull distributed clutter returns are present on a radar receiver and neither is dominant. Quantitative data is presented for several clutter types and false alarm probabilities