Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.     

HIRDES – The High-Resolution Double-Echelle Spectrograph for the World Space Observatory Ultraviolet (WSO/UV)

The World Space Observatory Ultraviolet (WSO/UV) is a multi-national project grown out of the needs of the astronomical community to have future access to the UV range. WSO/UV consists of a single UV telescope with a primary mirror of 1.7 m diameter feeding the UV spectrometer and UV imagers. The spectrometer comprises three different spectrographs, two high-resolution echelle spectrographs (the High-Resolution Double-Echelle Spectrograph, HIRDES) and a low-dispersion long-slit instrument. Within HIRDES the 102–310 nm spectral band is split to feed two echelle spectrographs covering the UV range 174–310 nm and the vacuum-UV range 102–176 nm with high spectral resolution (R > 50,000). The technical concept is based on the heritage of two previous ORFEUS SPAS missions. The phase-B1 development activities are described in this paper considering performance aspects, design drivers, related trade-offs (mechanical concepts, material selection etc.) and a critical functional and environmental test verification approach. The current state of other WSO/UV scientific instruments (imagers) is also described.     

Spectroscopy of laboratory plasmas

Work under the heading of Laboratory Plasma Spectroscopy may be conveniently separated into three classes depending on the extent to which the interaction of the emitting atoms with their plasma environment is central to the investigation. Zero order, the longest established use of laboratory plasmas in connection with astrophysics, concerns the use of hot plasmas for the excitation, measurement, and identification of the spectra of highly-stripped ions. In such work the properties of the plasma itself are usually of secondary importance. In first-order, plasma spectroscopy is used to determine fundamental atomic data concerned with the interaction of an atom with a single particle, usually either a photon or an electron, i.e.: the determination of oscillator strengths and collision cross-sections. Finally, higher-order processes in which the plasma nature of the surrounding medium is most relevant concern the study of line-shapes, and related topics such as the excitation of satellite spectral features by plasma oscillations. Developments in plasma diagnostic techniques in the last five years have greatly extended the scope of the second and third categories and have yielded much astrophysically important information from laboratory studies. Recent advances in these areas are reviewed.     

Composition of Interstellar Neutrals and the Origin of Anomalous Cosmic Rays

Knowledge of the elemental composition of the interstellar gas is of fundamental importance for understanding galactic chemical evolution. In addition to spectroscopic determinations of certain element abundance ratios, measurements of the composition of interstellar pickup ions and Anomalous Cosmic Rays (ACRs) have provided the principal means to obtain this critical information. Recent advances in our understanding of particle acceleration processes in the heliosphere and measurements by the Voyagers of the energy spectra and composition of energetic particles in the heliosheath provide us with another means of determining the abundance of the neutral components of the local interstellar gas. Here we compare the composition at the termination shock of six elements obtained from measurements of (a) pickup ions at ～5 AU, (b) ACRs in the heliosphere at ～70 AU, and (c) energetic particles as well as (d) ACRs in the heliosheath at ～100 AU. We find consistency among these four sets of derived neutral abundances. The average interstellar neutral densities at the termination shock for H, N, O, Ne and Ar are found to be 0.055±0.021 cm^?3, (1.44±0.45)×10^?5 cm^?3, (6.46±1.89)×10^?5 cm^?3, (8.5±3.3)×10^?6 cm^?3, and (1.08±0.49)×10^?7 cm^?3, respectively, assuming the He density is 0.0148±0.002 cm^?3.     

Performance Degradation Due to Specular Multipath Intersymbol Interference

Plots of performance degradation are used to compare the effects of fading and intersymbol interference in a two- component specular multipath digital communications channel. Similar plots are then used to compare two practical receivers designed to combat the interference. Degradation plots are shown to allow easy identification of each receiver's range of usefulness, as well as identification of variance bounds demanded of channel parameter estimates which the receivers require.     

Developing better artificial bones

Researchers at the Center for Commercial Applications of Combustion in Space at the Colorado School of Mines are preparing the Space-DRUMS (Dynamically Responding Ultrasonic Matrix Systems) materials processing facility for transport to the International Space Station. The Space-DRUMS uses acoustic pressure beams to maintain the position of a suspended liquid or solid. Space-DRUMS will be used to extend experiments with tricalcium phosphate in the development of artificial bone material.     

High-Resolution, Radar Coherent Linear FM Microwave Source

The microwave CLFM study was directed to generating 14 ?s S-band pulses of 1000 MHz bandwidth and an rms phase error of a few degrees. Over 972 MHz bandwidth, the sampled phase error relative to the reference was 7 degrees rms and 17 degrees peak, with a maximum Fourier component of 4 degrees. The FM pulse train is generated by a gated BWO driven by a stable linearizing waveform. Phase coherency during each pulse is obtained by a sampling technique, where the phase is corrected at intervals of 1/6 ?s, the RF phase having changed an integral number of cycles in each interval. Multiplication of the BWO signal by the sampling pulse train results in band-limited phase error pulses which are applied in a feedback loop. Pulse-to-pulse coherency is obtained by phase lock of the BWO starting frequency to the crystal reference. Feedback leveling holds the output constant to 0.3 dB. The basic MITRE technique was originally demonstrated at 10 MHz in 1964. Range results measured with the X-band model radar using the CLFM generator are given and confirm the phase errors of the CLFM.     

Radar Doppler Spectra of Turbulent Ionized Wakes

The evaluation of the double convolution integrat involved in the expression of the radar response to scattering from a turbulent ionized wake is simplified by the approximation technique presented here, so that the Doppler spectrum parameters can be explicitly expressed in terms of the wake characteristics.