Comparative survival analysis of Deinococcus radiodurans and the haloarchaea Natrialba magadii and Haloferax volcanii exposed to vacuum ultraviolet irradiation

The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10(5) to 10(6) cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10(-5) Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of (3.77±0.76)×10(-2), which was larger than that of D. radiodurans (1.13±0.23)×10(-2). The survival fraction of the haloarchaea H. volcanii, of (3.60±1.80)×10(-4), was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluences up to 150?J m(-2). For fluences larger than 150?J m(-2), there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher VUV fluence (1350?J m(-2)), while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable microorganisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and Jupiter's moon Europa. This is the first work to report survival of haloarchaea under simulated interplanetary conditions.     

Cross-correlation properties of algebraically constructed Costasarrays

The problem of determining the cross-correlation properties of signals based on algebraically constructed Costas arrays is addressed by examining the discrete cross-correlation of the algebraically constructed Costas arrays for a given construction and dimension. Finding two arrays that minimally correlate implies that the signals based on these arrays also minimally correlate. The properties of finite fields are reviewed, and the major algebraic constructions for Costas arrays are presented, i.e. the Welch construction and the Golomb construction. The discrete cross-correlation properties of the Costas arrays are derived for arrays of the same dimension derived from the same construction. The use of Costas arrays in the signal design problem is discussed, and examples are given to show the cross-correlation of the signals based on the algebraically constructed arrays     

Carrier-wave interference to Loran-C: a statistical evaluation

Loran-C is a pulsed, hyperbolic radio aid for long-range marine, air, and land navigation. The worldwide expansion of Loran-C, especially in Europe, has focused attention on carrier-wave interference to the system. The effects of a multiplicity of carrier-wave interferers (CWIs) on receiver timing measurement accuracy are considered. Both the phase tracking and the cycle-selection performance of receivers are quantified in terms of the probabilities that they will operate correctly within the arbitrarily set error limits. Characteristic functions (CFs) are used to compute these probabilities. Analyses and results are presented for typical situations. The relative sensitivity of the phase tracking and cycle-selection functions to interfering signals are discussed     

Comprehensive data reduction package for the Immersion GRating INfrared Spectrograph: IGRINS

We present a Python-based data reduction pipeline package (PLP) for the Immersion GRating INfrared Spectrograph (IGRINS), an instrument that covers the complete H- and K-bands in one exposure with a spectral resolving power of 40,000. The reduction steps carried out by the PLP include flat-fielding, background removal, order extraction, distortion correction, wavelength calibration, and telluric correction using spectra of A type standard stars. As the spectrograph has no moving parts, the PLP automatically reduces the data using predefined functions for the processes of order extraction, distortion correction, and wavelength calibration. Before the telluric correction of the target spectra, the intrinsic hydrogen absorption features of the standard A star are removed with a Gaussian fitting algorithm. The final result is the flux of the target as a function of wavelength. Users can customize the predefined functions for the extraction of the spectrum from the echellogram and adjust the parameters for the fitting functions for the spectra of celestial objects, using “fine-tuning” options, as necessary. Presently, the PLP produces the best results for point-source targets.     

A new perspective of carcinogenesis from protracted high-LET radiation arises from the two-stage clonal expansion model.

When applied to the Colorado Plateau miner population, the two-stage clonal expansion (TSCE) model of radiation carcinogenesis predicts that radiation-induced promotion dominates radiation-induced initiation. Thus, according to the model, at least for alpha-particle radiation from inhaled radon daughters, lung cancer induction over long periods of protracted irradiation appears to be dominated by radiation-induced modification of the proliferation kinetics of already-initiated cells rather than by direct radiation-induced initiation (i.e., mutation) of normal cells. We explore the possible consequences of this result for radiation exposures to space travelers on long missions. Still unknown is the LET dependence of this effect. Speculations of the cause of this phenomenon include the suggestion that modification of cell kinetics is caused by a "bystander" effect, i.e., the traversal of normal cells by alpha particles, followed by the signaling of these cells to nearby initiated cells which then modify their proliferation kinetics.     

Gravitropism of basidiomycetous fungi--on Earth and in microgravity.

In order to achieve perfect positioning of their lamellae for spore dispersal, fruiting bodies of higher fungi rely on the omnipresent force gravity. Only accurate negatively gravitropic orientation of the fruiting body cap will guarantee successful reproduction. A spaceflight experiment during the STS-55 Spacelab mission in 1993 confirmed that the factor gravity is employed for spatial orientation. Most likely every hypha in the transition zone between the stipe and the cap region is capable of sensing gravity. Sensing presumably involves slight sedimentation of nuclei which subsequently causes deformation of the net-like arrangement of F-actin filament strands. Hyphal elongation is probably driven by hormone-controlled activation and redistribution of vesicle traffic and vesicle incorporation into the vacuoles and cell walls to subsequently cause increased water uptake and turgor pressure. Stipe bending is achieved by way of differential growth of the flanks of the upper-most stipe region. After reorientation to a horizontal position, elongation of the upper flank hyphae decreases 40% while elongation of the lower flank slightly increases. On the cellular level gravity-stimulated vesicle accumulation was observed in hyphae of the lower flank.