DNA fragmentation by charged particle tracks.

High-LET (linear energy transfer) charged particles induce DNA double-strand breaks (DSB) in a non-random fashion in mammalian cells. The clustering of DSB, probably determined by track structure as well as chromatin conformation, results in an excess of small- and intermediate-sized DNA fragments. DNA fragmentation in normal human fibroblasts (GM5758) was analyzed by pulsed-field gel electrophoresis after irradiation with photons (60Co) or 125 keV/micrometers nitrogen ions. Compared to conventional DSB analysis, i.e. assays only measuring the fraction of DNA smaller than a single threshold, the relative biological effectiveness (RBE) for DSB induction increased with 100%. Further, the size distribution of DNA fragments showed a significant dependence on radiation quality, with an excess of fragments up to 1 Mbp. Irradiation of naked genomic DNA without histone proteins increased the DSB yields 25 and 13 times for photons and nitrogen ions, respectively. The results suggest possible roles of both track structure and chromatin organization in the distribution of DNA double-strand breaks along the chromosome.     

Issues of exploration: human health and wellbeing during a mission to Mars.

Today, the tools are in our hands to enable us to travel away from our home planet and become citizens of the solar system. Even now, we are seriously beginning to develop the robust infrastructure that will make the 21st century the Century of Space Travel. But this bold step must be taken with due concern for the health, safety and wellbeing of future space explorers. Our long experience with space biomedical research convinces us that, if we are to deal effectively with the medical and biomedical issues of exploration, then dramatic and bold steps are also necessary in this field. We can no longer treat the human body as if it were composed of muscles, bones, heart and brain acting independently. Instead, we must lead the effort to develop a fully integrated view of the body, with all parts connected and fully interacting in a realistic way. This paper will present the status of current (2000) plans by the National Space Biomedical Research Institute to initiate research in this area of integrative physiology and medicine. Specifically, three example projects are discussed as potential stepping stones towards the ultimate goal of producing a digital human. These projects relate to developing a functional model of the human musculoskeletal system and the heart.     

Gravisensing: ionic responses, cytoskeleton and amyloplast behavior.

In Zea mays L., changes in orientation of stems are perceived by the pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. Gravity is perceived in the bundle sheath cells, which contain amyloplasts that sediment to the new cell base when a change in the gravity vector occurs. The mechanism by which the mechanical signal is transduced into a physiological response is so far unknown for any gravity perceiving tissue. It is hypothesized that this involves interactions of amyloplasts with the plasma membrane and/or ER via cytoskeletal elements. To gain further insights into this process we monitored amyloplast movements in response to gravistimulation. In a pharmacological approach we investigated how the dynamics of plastid sedimentation are affected by actin and microtubule (MT) disrupting drugs. Dark grown caulonemal filaments of the moss Physcomitrella patens respond to gravity vector changes with a reorientation of tip growth away from the gravity vector. MT distributions in tip cells were monitored over time and MTs were seen to accumulate preferentially on the lower flank of the tip 30 min after a 90 degree turn. Using a self-referencing Ca2+ selective ion probe, we found that growing caulonemal filaments exhibit a Ca2+ influx at the apical dome, similar to that reported previously for other tip growing cells. However, in gravistimulated Physcomitrella filaments the region of Ca2+ influx is not confined to the apex, but extends about 60 micrometers along the upper side of the filament. Our results indicate an asymmetry in the Ca2+ flux pattern between the upper and side of the filament suggesting differential activation of Ca2+ permeable channels at the plasma membrane.     

Indirect search for Dark Matter with H.E.S.S.

Observations of the Galactic center region with the H.E.S.S. telescopes have established the existence of a steady, extended source of gamma-ray emission coinciding with the position of the super massive black hole Sgr A^*. This is a remarkable finding given the expected presence of dense self-annihilating Dark Matter in the Galactic center region. The self-annihilation process is giving rise to gamma-ray production through hadronization including the production of neutral pions which decay into gamma-rays but also through (loop-suppressed) annihilation into final states of almost mono-energetic photons. We study the observed gamma-ray signal (spectrum and shape) from the Galactic center in the context of Dark Matter annihilation and indicate the prospects for further indirect Dark Matter searches with H.E.S.S.     

Hermes vehicle

The presence of Europe in the future developments of spatial programs, which are foreseen, for the 1990s and further, needs the availability of vehicles, modules and all related technologies adapted to operational use of low earth orbit station.The manned HERMES vehicle shall be part of the in-orbit infrastructure realized either in the European context or in cooperation between Europe and the United States.The main mission for this vehicle will be to run a shuttle with the station that means transport and change of the crews, its safe return in abort condition and cargo transport of consumable and experimental equipment.Secondary missions could be servicing on automatic platform, making autonomous scientific experiments. Lastly, the vehicle, by means of its on-board propulsion capability, could be used to accomplish in-orbit tow and assembly missions.Studies which are undertaken now about the vehicle are devoted to the aerodynamic shape (research of a compromise between aerothermic and overall fitting), the system (functional architecture, ground and flight configuration); further works dealing with technology are presently on hand in the field of thermal protection, aerodynamics, power generation with a high massic yield.     

A space radiation transport method development.

Improved spacecraft shield design requires early entry of radiation constraints into the design process to maximize performance and minimize costs. As a result, we have been investigating high-speed computational procedures to allow shield analysis from the preliminary design concepts to the final design. In particular, we will discuss the progress towards a full three-dimensional and computationally efficient deterministic code for which the current HZETRN evaluates the lowest-order asymptotic term. HZETRN is the first deterministic solution to the Boltzmann equation allowing field mapping within the International Space Station (ISS) in tens of minutes using standard finite element method (FEM) geometry common to engineering design practice enabling development of integrated multidisciplinary design optimization methods. A single ray trace in ISS FEM geometry requires 14 ms and severely limits application of Monte Carlo methods to such engineering models. A potential means of improving the Monte Carlo efficiency in coupling to spacecraft geometry is given in terms of re-configurable computing and could be utilized in the final design as verification of the deterministic method optimized design.     

The electrical Environment of the Earth's Atmosphere: A Review

The study of the electrical environment of the Earth's atmosphere has rapidly advanced during the past century. Great strides have been made towards the understanding of lightning and thunderstorms and in relating them to the global electric circuit. The electromagnetic fields and currents connect different parts of the Earth's environment, and any type of perturbation in one region affects another region. Starting from the traditional views in which the electrodynamics of one region has been studied in isolation from the neighboring regions, the modern theory of the global electrical circuit has been discussed briefly. Interconnection and electrodynamic coupling of various regions of the Earth's environment can be easily studied by using the global electric circuit model. Deficiencies in the model and the possibility of improvement in it have been suggested. Application of the global electric circuit model to the understanding of the Earth's changes of climate has been indicated.     

Indicators of black-hole mass and Eddington accretion ratio from QSO X-ray and UV spectra

The evolution of luminous QSOs is linked to the evolution of massive galaxies. We know this because the relic black-holes found locally have masses dependent on the properties of the host galaxy’s bulge. An important way to explore this evolution would be to measure dependences of black-hole masses and Eddington accretion ratios over a range of redshifts, i.e., with cosmological age. For low redshift QSOs (and their lower luminosity Seyfert galaxy counterparts) it has been possible to infer black-hole masses from the luminosities and velocity dispersions of their host-galaxy bulges. These masses agree with those virial black-hole masses calculated from the Doppler widths of the broad Hβ emission lines. The latter method can then be extended to more distant and luminous QSOs, up to redshifts of 0.6 with ground-based optical observations. We discuss ways to extend these explorations to higher redshifts – up to 3 using the widths of QSOs’ broad UV emission lines, and in principle, and to redshifts near 4 from ground-based infrared observations of rest-frame Hβ at 2.5 μm. We discuss the possibility of investigating the accretion history of the higher redshift QSOs using measures of Eddington accretion ratio – the soft X-ray spectral index and the eigenvectors of Principal Components Analyses of QSOs’ UV emission-line spectra.     

Linear parametrically varying systems with brief instabilities: an application to vision/inertial navigation

This paper addresses the problem of nonlinear filter design to estimate the relative position and velocity of an unmanned air vehicle (UAV) with respect to a point on a ship using infrared (IR) vision, inertial, and air data sensors. Sufficient conditions are derived for the existence of a particular type of complementary filters with guaranteed stability and performance in the presence of so-called out-of-frame events that arise when the vision system loses its target temporarily. The results obtained build upon new developments in the theory of linear parametrically varying systems (LPVs) with brief instabilities - also reported in the paper - and provide the proper framework to deal with out-of-frame events. Field tests with a prototype UAV illustrate the performance of the filter and the scope of applications of the new theory developed.