Mammalian cell cultivation in space.

Equipment used in space for the cultivation of mammalian cells does not meet the usual standard of earth bound bioreactors. Thus, the development of a space worthy bioreactor is mandatory for two reasons: First, to investigate the effect on single cells of the space environment in general and microgravity conditions in particular, and second, to provide researchers on long term missions and the Space Station with cell material. However, expertise for this venture is not at hand. A small and simple device for animal cell culture experiments aboard Spacelab (Dynamic Cell Culture System; DCCS) was developed. It provides 2 cell culture chambers, one is operated as a batch system, the other one as a perfusion system. The cell chambers have a volume of 200 microliters. Medium exchange is achieved with an automatic osmotic pump. The system is neither mechanically stirred nor equipped with sensors. Oxygen for cell growth is provided by a gas chamber that is adjacent to the cell chambers. The oxygen gradient produced by the growing cells serves to maintain the oxygen influx by diffusion. Hamster kidney cells growing on microcarriers were used to test the biological performance of the DCCS. On ground tests suggest that this system is feasible.     

Determination of aerosol optical depth from ground measurements

The methods used to determine the aerosol optical depth as a function of wavelength are briefly described and discussed. Some results from the operational network of the World Meteorological Organization and other, more research oriented studies, are reviewed and critically analysed to assess the reliability and accuracy of such determinations and their value as ground truth measurements for space applications.     

Heterodyne spectroscopy of astronomical and laboratory sources at 8.5 μm using diode laser local oscillators

The first 8.5m infrared heterodyne spectrometer has been constructed using tuneable semiconductor (PbSe) diode lasers and was used to measure absorption line profiles of N₂O in the laboratory and black body emission from the Moon and from Mars. Spectral information was recorded over a 200 MHz bandwidth using an 8-channel filter bank. The resolution was 25 MHz (6 × 10^–6 m) and the minimum detectable (black-body) power was 1 × 10^–16 W for 8 min of integration. The results demonstrate the usefulness of heterodyne spectroscopy for the study of remote and local sources in the infrared.We wish to thank Dr Jack Butler and Craig Simpson (Arthur D. Little, Inc.) for their continuing (and successful) efforts to grow better diode lasers during the course of this experiment. We thank the National Radio Astronomy Observatory for the loan of a 40-channel filter bank and associated mini-computer.We thank en masse the dozens of people who loaned equipment to us for the duration of this experiment, and the commercial firms (especially Harshaw, Inc.) which expedited orders for crucial equipment. We thank Dr Bertram Donn, Dr Norman Ness, and Dr George Pieper for administrative, scientific, and financial support; and we thank Mr James Faris for technical assistance and Dr Sol Glicker for preparation of sample gas cells. We thank Dr Thomas Clark for providing the Kohoutek ephemeris, sighting printout, and predicted molecular line positions and for advice and the loan of some equipment. Finally, we wish to acknowledge the strong support accorded us by our wives and families who scarcely saw us during this period.     

The Diverse Planetary Ingassing/Outgassing Paths Produced over Billions of Years of Magmatic Activity

Space Science Reviews - Accretion onto black holes is an efficient mechanism in converting the gas mass-energy into energetic outputs as radiation, wind and jet. Tidal disruption events, in which...     

Mercury Dust Monitor (MDM) Onboard the Mio Orbiter of the BepiColombo Mission

Space Science Reviews - In this chapter, we review the contribution of space missions to the determination of the elemental and isotopic composition of Earth, Moon and the terrestrial planets, with...     

Genesis and preservation of a uranium-rich paleozoic epithermal system with a surface expression (Northern Flinders Ranges, South Australia): radiogenic heat driving regional hydrothermal circulation over geological timescales

The surface expressions of hydrothermal systems are prime targets for astrobiological exploration, and fossil systems on Earth provide an analogue to guide this endeavor. The Paleozoic Mt. Gee-Mt. Painter system (MGPS) in the Northern Flinders Ranges of South Australia is exceptionally well preserved and displays both a subsurface quartz sinter (boiling horizon) and remnants of aerial sinter pools that lie in near-original position. The energy source for the MGPS is not related to volcanism but to radiogenic heat produced by U-Th-K-rich host rocks. This radiogenic heat source drove hydrothermal circulation over a long period of time (hundreds of millions of years, from Permian to present), with peaks in hydrothermal activity during periods of uplift and high water supply. This process is reflected by ongoing hot spring activity along a nearby fault. The exceptional preservation of the MGPS resulted from the lack of proximal volcanism, coupled with tectonics driven by an oscillating far-field stress that resulted in episodic basement uplift. Hydrothermal activity caused the remobilization of U and rare earth elements (REE) in host rocks into (sub)economic concentrations. Radiogenic-heat-driven systems are attractive analogues for environments that can sustain life over geological times; the MGPS preserves evidence of episodic fluid flow for the past ～300 million years. During periods of reduced hydrothermal activity (e.g., limited water supply, quiet tectonics), radiolytic H(2) production has the potential to support an ecosystem indefinitely. Remote exploration for deposits similar to those at the MGPS systems can be achieved by combining hyperspectral and gamma-ray spectroscopy.     

Kinematics of Saturn's spokes

Voyager 2 images of Saturn's rings have been analyzed for spoke activity. More than 80 and 40 different spokes have been measured at the morning and at the evening ansa, respectively. Higher rate of spoke formation has been found at 145° ± 15° SLS and at 305° ± 15° SLS which persisted for at least 3 Saturn revolutions. Higher spoke activity (formation and growth in width) by more than a factor 3 has been observed over the nightside hemisphere of Saturn than over the dayside hemisphere. The age distribution (i.e. time from radial formation until observation, assuming Keplerian shear) of the leading (old) edges of spokes has its maximum at ～ 9,000 s and ～ 6,000 s for spokes observed at the morning ansa and at the evening ansa, respectively. The highest spoke age observed is ～ 20,000 s. The age distribution of the trailing (young) edges of spokes peaks at < 2,000 s at both ansae but has its mean at ～ 4,500 s and ～ 3,500 s, respectively. On the average the observed spokes grew in width for ～ 4,500 s at the morning ansa and for ～ 2,500 s at the evening ansa. The maximum time of growth in width was ～ 12,000 s.     

Thermal model and thermo-mechanical stresses in cometary nuclei

Spherically symmetric radial temperature profiles of cometary nuclei have been determined numerically (and for simplified models analytically) in dependence on the orbital position of the periodic comet Halley. These temperature fields in the nucleus are connected with thermal stress fields which have been calculated with the assumption of elastic properties of cometary matter. The remarkable result is the possible existence of stresses, strong enough to cause internal cracking of the nucleus and break-ups of the cometary surface. This may be essential understanding normal cometary activity as well as outbursts and splits.     

Solution numérique du problème de Képler

A numerical solution of the Kepler problem. Requiring a six-decimal accuracy, the Kepler problem can be solved with four iterations at most, even for an eccentricity close to 1, provided that a new initial approximation be introduced in this last case.