Abundance and diversity of microbial inhabitants in European spacecraft-associated clean rooms

The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA's and ESA's guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heat-resistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods.     

Modified energy cascade model adapted for a multicrop Lunar greenhouse prototype

Models are required to accurately predict mass and energy balances in a bioregenerative life support system. A modified energy cascade model was used to predict outputs of a multi-crop (tomatoes, potatoes, lettuce and strawberries) Lunar greenhouse prototype. The model performance was evaluated against measured data obtained from several system closure experiments. The model predictions corresponded well to those obtained from experimental measurements for the overall system closure test period (five months), especially for biomass produced (0.7% underestimated), water consumption (0.3% overestimated) and condensate production (0.5% overestimated). However, the model was less accurate when the results were compared with data obtained from a shorter experimental time period, with 31%, 48% and 51% error for biomass uptake, water consumption, and condensate production, respectively, which were obtained under more complex crop production patterns (e.g. tall tomato plants covering part of the lettuce production zones). These results, together with a model sensitivity analysis highlighted the necessity of periodic characterization of the environmental parameters (e.g. light levels, air leakage) in the Lunar greenhouse.     

Analysis of data of “Clementine” and “KAGUYA” missions and “ULCN” and “KSC-1162” catalogues

In this paper an analysis of data coordinate systems from selenographic catalogues and space missions was carried out. The lunar macrorelief models were made on basis of the software package ASNI USTU using method of the spherical harmonic expansion. These models accurately describe the global features of the lunar figure. To construct these models the following sources of topographic information were used: “Clementine” and “KАGUYА” (Selena, Japan mission) missions, “KSC-1162” (Kazan selenocentric catalogue), “Kiev” (selenodesic catalogue), “SAI” (Chuikova (1975)), “Bills, Ferrari”, “ULCN” (The Unified Lunar Control Network 2005). Direct comparison hypsometric information “KSС-1162” catalogue data with “Clementine” mission was carried out. These researches confirmed a good agreement of the hypsometric information of compared systems. The normalized coefficients were obtained on basis of the hypsometric information expansion for eight sources. The displacement of the lunar center of mass (LCM) relatively to the lunar center of figure (LCF) was obtained by using topographic data selenodetical catalogues and space missions.     

Soybean cultivar selection for Bioregenerative Life Support Systems (BLSSs) – Hydroponic cultivation

Four soybean cultivars (‘Atlantic’, ‘Cresir’, ‘Pr91m10’ and ‘Regir’), selected through a theoretical procedure as suitable for cultivation in BLSS, were evaluated in terms of growth and production. Germination percentage and Mean Germination Time (MGT) were measured. Plants were cultivated in a growth chamber equipped with a recirculating hydroponic system (Nutrient Film Technique). Cultivation was performed under controlled environmental conditions (12 h photoperiod, light intensity 350 μmol m⁻² s⁻¹, temperature regime 26/20 °C light/dark, relative humidity 65–75%). Fertigation was performed with a standard Hoagland solution, modified for soybean specific requirements, and EC and pH were kept at 2.0 dS m⁻¹ and 5.5 respectively.     

Vertical shear at the equatorial F-region ionosphere during post-sunset hours

The characteristics of the equatorial F-region zonal plasma drift during post-sunset period have been investigated using the multi-frequency HF Doppler radar. The pattern of the zonal plasma drift is such that it starts with a westward drift during the pre-sunset hours, followed by an eastward drift shortly after the E-region sunset. The zonal plasma drift is characterized by the presence of a positive vertical shear around the post-sunset period and maximum shear is observed at the time of the peak of the pre-reversal enhancement in the vertical drift. The presence of vertical shear in the zonal drift is associated with the post-sunset velocity vortex existing at the equatorial F-region.     

Removing orbital debris with lasers

Orbital debris in low Earth orbit (LEO) are now sufficiently dense that the use of LEO space is threatened by runaway collision cascading. A problem predicted more than thirty years ago, the threat from debris larger than about 1 cm demands serious attention. A promising proposed solution uses a high power pulsed laser system on the Earth to make plasma jets on the objects, slowing them slightly, and causing them to re-enter and burn up in the atmosphere. In this paper, we reassess this approach in light of recent advances in low-cost, light-weight modular design for large mirrors, calculations of laser-induced orbit changes and in design of repetitive, multi-kilojoules lasers, that build on inertial fusion research. These advances now suggest that laser orbital debris removal (LODR) is the most cost-effective way to mitigate the debris problem. No other solutions have been proposed that address the whole problem of large and small debris. A LODR system will have multiple uses beyond debris removal. International cooperation will be essential for building and operating such a system.     

Low-latitude ionospheric scintillations and total electron content obtained with the CITRIS instrument on STPSat1 using radio transmissions from DORIS ground beacons

The primary objective of the Scintillation and Tomography Receiver in Space (CITRIS) is to detect ionospheric irregularities from space at low latitude. For this purpose, the satellite receiver uses the UHF and S-Band transmissions of the ground network of Doppler Orbitography and Radiopositioning Integrated by Satellite (DORIS) beacons. CITRIS, developed at the Naval Research Laboratory, differs from the normal DORIS receiver by being able to capture and store the complex amplitude of the 401.25 and 2036.25 MHz transmissions at 200 Hz sample rate. Ground processing of the CITRIS data yields total electron content (TEC) and both phase and amplitude scintillations. With CITRIS flying on the US Space Test Program (STP) satellite STPSat1, 2 years of data were collected and processed to determine the fluctuations in ionospheric TEC and radio scintillations associated with equatorial irregularities. CITRIS flights over DORIS transmitters yield direct measurements of the horizontal plasma density fluctuations associated with equatorial plasma bubbles. Future flights of CITRIS can provide valuable complements to other satellite instruments such as GPS occultation receivers used to estimate vertical electron density profiles in the ionosphere.     

Assessment of the NeQuick model at mid-latitudes using GNSS TEC and ionosonde data

The modelling of the total electron content (TEC) plays an important role in global navigation satellite systems (GNSS) accuracy, especially for single-frequency receivers, the most common ones constituting the mass market. For the latter and in the framework of Galileo, the NeQuick model has been chosen for correcting the ionospheric error contribution and will be integrated into a global algorithm providing the users with daily updated information.