Setting a standard: the limulus amebocyte lysate assay and the assessment of microbial contamination on spacecraft surfaces

Historically, colony-forming units as determined by plate cultures have been the standard unit for microbiological analysis of environmental samples, medical diagnostics, and products for human use. However, the time and materials required make plate cultures expensive and potentially hazardous in the closed environments of future NASA missions aboard the International Space Station and missions to other Solar System targets. The Limulus Amebocyte Lysate (LAL) assay is an established method for ensuring the sterility and cleanliness of samples in the meat-packing and pharmaceutical industries. Each of these industries has verified numerical requirements for the correct interpretation of results from this assay. The LAL assay is a rapid, point-of-use, verified assay that has already been approved by NASA Planetary Protection as an alternate, molecular method for the examination of outbound spacecraft. We hypothesize that standards for molecular techniques, similar to those used by the pharmaceutical and meat-packing industries, need to be set by space agencies to ensure accurate data interpretation and subsequent decision making. In support of this idea, we present research that has been conducted to relate the LAL assay to plate cultures, and we recommend values obtained from these investigations that could assist in interpretation and analysis of data obtained from the LAL assay.     

Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.     

Solar Wind Turbulence and the Role of Ion Instabilities

Solar wind is probably the best laboratory to study turbulence in astrophysical plasmas. In addition to the presence of magnetic field, the differences with neutral fluid isotropic turbulence are: (i) weakness of collisional dissipation and (ii) presence of several characteristic space and time scales. In this paper we discuss observational properties of solar wind turbulence in a large range from the MHD to the electron scales. At MHD scales, within the inertial range, turbulence cascade of magnetic fluctuations develops mostly in the plane perpendicular to the mean field, with the Kolmogorov scaling $k_{\perp}^{-5/3}$ for the perpendicular cascade and $k_{\|}^{-2}$ for the parallel one. Solar wind turbulence is compressible in nature: density fluctuations at MHD scales have the Kolmogorov spectrum. Velocity fluctuations do not follow magnetic field ones: their spectrum is a power-law with a ?3/2 spectral index. Probability distribution functions of different plasma parameters are not Gaussian, indicating presence of intermittency. At the moment there is no global model taking into account all these observed properties of the inertial range. At ion scales, turbulent spectra have a break, compressibility increases and the density fluctuation spectrum has a local flattening. Around ion scales, magnetic spectra are variable and ion instabilities occur as a function of the local plasma parameters. Between ion and electron scales, a small scale turbulent cascade seems to be established. It is characterized by a well defined power-law spectrum in magnetic and density fluctuations with a spectral index close to ?2.8. Approaching electron scales, the fluctuations are no more self-similar: an exponential cut-off is usually observed (for time intervals without quasi-parallel whistlers) indicating an onset of dissipation. The small scale inertial range between ion and electron scales and the electron dissipation range can be together described by $\sim k_{\perp}^{-\alpha}\exp(-k_{\perp}\ell_{d})$ , with α?8/3 and the dissipation scale ? _d close to the electron Larmor radius ? _d ?ρ _e . The nature of this small scale cascade and a possible dissipation mechanism are still under debate.     

From TOPEX/Poseidon to Jason-2/OSTM in the Amazon basin

A major interest of radar altimetry over rivers is to monitor water resources and associated risk in basins where there is little or no conventional in situ data. The objective of the present study is to calibrate altimetry data in a place where conventional data are available, and use the results to estimate the potential error committed in the estimation of water levels in an ungauged or poorly gauged basin. The virtual stations extracted with Jason-2 in this study concern a very broad sample of river channel width and complexity. Minimum channel width has been estimated at 400 m. Unlike TOPEX/Poseidon (T/P), Jason-2 seems to have the capability to distinguish the river bed from its floodplain. The quality of the results obtained with Jason-2 is incomparably better than that obtained with T/P. Despite the fact that no absolute calibration has been assessed for river in this study, the bias calculated converge around 0, 35 m, which could be then the error estimated on the water stage derived from Jason-2 ranges, when no other validation is available. ICE3 algorithm seems to be performing as well as ICE1, and further research is needed to design retracking algorithm specifically for continental water.     

Improved interpretation of solar wind ion measurements via high-resolution magnetic field data

The Wind   spacecraft’s Faraday cups (FC) continue to produce high-quality, in situ observations of thermal protons (i.e., ionized hydrogen) and α$\alpha$-particles (i.e., fully ionized helium) in the solar wind. By fitting a Wind/FC ion spectrum with a model velocity distribution function (VDF) for each particle species, values for density, bulk velocity, and temperature can be inferred. Incorporating measurements of the background magnetic field from the Wind Magnetic Field Investigation (MFI) allows perpendicular and parallel temperature components to be separated. Prior implementations of this analysis averaged the higher-cadence Wind/MFI measurements to match that of the Wind/FC ion spectra. However, this article summarizes recent and extensive revisions to the analysis software that, among other things, eliminate such averaging and thereby account for variations in the direction of the magnetic field over the time taken to measure the ions. A statistical comparison reveals that the old version consistently underestimates the temperature anisotropy of ion VDF’s: averaging over fluctuations in the magnetic field essentially blurs the perpendicular and parallel temperature components, which makes the plasma seem artificially more isotropic. The new version not only provides a more accurate dataset of ion parameters (which is well suited to the study of microkinetic phenomena), it also demonstrates a novel technique for jointly processing particle and field data. Such methods are crucial to heliophysics as wave-particle interactions are increasingly seen as playing an important role in the dynamics of the solar wind and similar space plasmas.     

Height of shock formation in the solar corona inferred from observations of type II radio bursts and coronal mass ejections

Employing coronagraphic and EUV observations close to the solar surface made by the Solar Terrestrial Relations Observatory (STEREO) mission, we determined the heliocentric distance of coronal mass ejections (CMEs) at the starting time of associated metric type II bursts. We used the wave diameter and leading edge methods and measured the CME heights for a set of 32 metric type II bursts from solar cycle 24. We minimized the projection effects by making the measurements from a view that is roughly orthogonal to the direction of the ejection. We also chose image frames close to the onset times of the type II bursts, so no extrapolation was necessary. We found that the CMEs were located in the heliocentric distance range from 1.20 to 1.93 solar radii (Rs), with mean and median values of 1.43 and 1.38 Rs, respectively. We conclusively find that the shock formation can occur at heights substantially below 1.5 Rs. In a few cases, the CME height at type II onset was close to 2 Rs. In these cases, the starting frequency of the type II bursts was very low, in the range 25–40 MHz, which confirms that the shock can also form at larger heights. The starting frequencies of metric type II bursts have a weak correlation with the measured CME/shock heights and are consistent with the rapid decline of density with height in the inner corona.     

Heliotropic dust rings for Earth climate engineering

This paper examines the concept of a Sun-pointing elliptical Earth ring comprised of dust grains to offset global warming. A new family of non-Keplerian periodic orbits, under the effects of solar radiation pressure and the Earth’s J₂ oblateness perturbation, is used to increase the lifetime of the passive cloud of particles and, thus, increase the efficiency of this geoengineering strategy. An analytical model is used to predict the orbit evolution of the dust ring due to solar-radiation pressure and the J₂ effect. The attenuation of the solar radiation can then be calculated from the ring model. In comparison to circular orbits, eccentric orbits yield a more stable environment for small grain sizes and therefore achieve higher efficiencies when the orbit decay of the material is considered. Moreover, the novel orbital dynamics experienced by high area-to-mass ratio objects, influenced by solar radiation pressure and the J₂ effect, ensure the ring will maintain a permanent heliotropic shape, with dust spending the largest portion of time on the Sun facing side of the orbit. It is envisaged that small dust grains can be released from a circular generator orbit with an initial impulse to enter an eccentric orbit with Sun-facing apogee. Finally, a lowest estimate of 1 × 10¹² kg of material is computed as the total mass required to offset the effects of global warming.     

Symmetry and asymmetry of ionospheric weather at magnetic conjugate points for two midlatitude observatories

Variations of the ionospheric weather W-index for two midlatitude observatories, namely, Grahamstown and Hermanus, and their conjugate counterpart locations in Africa are studied for a period from October 2010 to December 2011. The observatories are located in the longitude sector, which has consistent magnetic equator and geographic equator so that geomagnetic latitudes of the line of force are very close to the corresponding geographic latitudes providing opportunity to ignore the impact of the difference of the gravitational field and the geomagnetic field at the conjugate points on the ionosphere structure and dynamics. The ionosondes of Grahamstown and Hermanus provide data of the critical frequency (foF2), and Global Ionospheric Maps (GIM) provide the total electron content (TECgps) along the magnetic field line up to the conjugate point in the opposite hemisphere. The global model of the ionosphere, International Reference Ionosphere, extended to the plasmasphere altitude of 20,200 km (IRI-Plas) is used to deliver the F2 layer peak parameters from TECgps at the magnetic conjugate area. The evidence is obtained that the electron gas heated by day and cooled by night at the summer hemisphere as compared with the opposite features in the conjugate winter hemisphere testifies on a reversal of plasma fluxes along the magnetic field line by the solar terminator. The ionospheric weather W-index is derived from NmF2 (related with foF2) and TECgps data. It is found that symmetry of W-index behavior in the magnetic conjugate hemispheres is dominant for the equinoxes when plasma movement along the magnetic line of force is imposed on symmetrical background electron density and electron content. Asymmetry of the ionospheric storm effects is observed for solstices when the plasma diffuse down more slowly into the colder winter hemisphere than into the warmer summer hemisphere inducing either plasma increase (positive phase) or decrease (negative phase of W-index) in the ionospheric and plasmaspheric plasma density.     

Performance evaluation of IRI-2007 at equatorial latitudes and its Matlab version for GNSS applications

International Reference Ionosphere (IRI) model is the widely used empirical model for ionospheric predictions, especially TEC which is an important parameter for radio navigation and communication. The Fortran based IRI-2007 does not support real-time interactive visualization and debugging. Therefore, the source code is converted into Matlab and is validated for the purposes of this study. This facilitates easy representation of results and for near real-time implementation of IRI in the applications including spacecraft launching, now casting, pseudolite based navigation systems etc. In addition, the vertical delay results over the equatorial region derived from IRI and GPS data of three IGS stations namely Libreville (Garbon, Africa), Brasilia (Brazil, South America) and Hyderabad (India, Asia) are compared. As the IRI model does not account for plasmasphere TEC, the vertical delays are underestimated compared to vertical delays of GPS signals. Therefore, the model should be modified accordingly for precise TEC estimation.