UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

Physical properties of dust grains deduced by optical probing techniques

In-situ space observations of dust in the solar system are seldom possible. On the opposite, remote observations of solar light scattered by dust are relatively easy to perform from Earth- or satellite-based observatories; the evolution of the polarization of light scattered by dust particles as a function of the phase angle may provide information on the physical properties of these particles. Unfortunately, since remote observations are integrated along the line-of-sight of the observer, they can hardly be used to determine local physical properties. We have precisely developed Optical Probe techniques to forge the link between the numerous remote observations and the unique in-situ measurements. A short review of the remote observations of light scattered by cometary dust is first presented. Then, the Optical Probe concept is analyzed. Finally, the OPE instrument, which had been designed to optically probe the inner coma of comet Halley is described; its limitations and its achievements during Halley and Grigg-Skjellerup encounters are discussed.     

Evaluation of Cyanothece sp. ATCC 51142 as a candidate for inclusion in a CELSS.

Controlled ecological life support systems (CELSS) have been proposed to make long-duration manned space flights more cost-effective. Higher plants will presumably provide food and a breathable atmosphere for the crew. It has been suggested that imbalances between the CO2/O2 gas exchange ratios of the heterotrophic and autotrophic components of the system will inevitably lead to an unstable system, and the loss of O2 from the atmosphere. Ratio imbalances may be corrected by including a second autotroph with an appropriate CO2/O2 gas exchange ratio. Cyanothece sp. ATCC 51142 is a large unicellular N2-fixing cyanobacterium, exhibiting high growth rates under diverse physiological conditions. A rat-feeding study showed the biomass to be edible. Furthermore, it may have a CO2/O2 gas exchange ratio that theoretically can compensate for ratio imbalances. It is suggested that Cyanothece spp. could fulfill several roles in a CELSS: supplementing atmosphere recycling, generating fixed N from the air, providing a balanced protein supplement, and protecting a CELSS in case of catastrophic crop failure.     

Dynamic considerations for control of closed life support systems

Reliability of closed life support systems will depend on their ability to continue supplying the crew's needs in the face of perturbations and equipment failures. These dynamic considerations interact with the basic static (equilibrium) design through the sizing of storages, the specification of excess capacities in processors, and the choice of system initial state (total mass in the system). This paper uses a very simple system flow model to examine the possibilities for system failures even when there is sufficient storage to buffer the immediate effects of the perturbation. Two control schemes are shown which have different dynamic consequences in response to component failures.     

The influence of gravity on the structure and functions of plant material

Growth process generate plant form and relate to most physiological functions. The Earth's gravity force affects plant growth in both obvious and subtle ways. It is a major environmental influence on morphology and physiology of plants. Gravity is less important as an agent for plant stress than as an environmental signal to guide growth. The plant's bioaccelerometers are remarkably sensitive, especially in hypogravity. Simulation (clinostat) studies and experiments in satellite laboratories are needed to understand the sensing, transduction, and response characteristics of g related mechanisms. By examining how plants alter growth processes to accomplish developmental or physiological “objectives” we may find it pragmatically desirable to ask ourselves how we might design a plant to achieve such responses to environmental influences. Examples of this design engineering approach for gravity related effects are described as an aid to experimentation.     

New multifrequency measurements of the spectrum of the cosmic background radiation

New measurements of the Cosmic Background Radiation temperature at 12 cm, 6.3 cm, 3 cm, 0.9 cm and 0.3 cm have made in July 1982 from the White Mountain High Altitude Research Station. The results are presented and the existence of spectral distortions discussed.     

Survey of earth orbital telescopes and their potential for exobiology

A series of Workshops on Exobiology in Earth Orbit held at NASA Ames Research Center has recently concluded. The draft of the final report from these Workshops contains a prioritized list of telescopic observations (possible only from above the Earth's atmosphere) that relate to the origin and evolution of the biogenic elements and compounds from their nucleosynthetic creation within stars to their inclusion in living systems. These orbital observations and the ground based laboratory and theoretical research necessary to support them have been termed Observational Exobiology. The details available on spacecraft, platforms and instrumentation most likely to be launched in the near future by the U.S. and Europe were considered in the Workshops. The purpose was to determine what observational programs would be tractible and what area of interest to exobiology required hardware and/or mission capabilities not yet envisioned. This paper summarizes the exciting opportunities that exist for Observational Exobiology.     

Biological life support for manned missions by ESA.

The anticipated evolution of life support technologies for ESA, considering both the complementary life support system requirements and the missions' characteristics, is presented. Based on these results, promising biological life support technologies for manned space missions have been selected by ESA either for their intrinsic ability and performance in effecting specific tasks for atmosphere-, water-, waste-management versus physico-chemical alternatives and/or for longer-term application to a more ecological concept (CES) focusing ultimately on food production. Actual status and plan for terrestrial and space testing of biological life support presented focusing on the "task specific" decontamination technology of the Biological Air Filter (BAF), and on food reprocessing technologies from biodegradable wastes with the MELISSA microbial ecosystem.     

Wide field cameras for SAX

SAX is an Italian X-ray satellite with a Dutch contribution that will be placed in orbit in 1994. The prime scientific object of SAX is to cover an energy bandwidth that ranges from 0.1 keV up to 200 keV. Among other instruments, SAX will consist of two X-ray Wide Field Cameras built by the Space Research Organisation Netherlands at Utrecht. The WFCs are based on the coded mask principle, the reconstruction of the image takes place on ground. The field of view is 20 degrees square full width at half maximum (FWHM), the angular resolution 5 arcminutes (FWHM) and the energy band ranges from 1.8 to 30 keV with a resolution of 18% at 6 keV. The sensitive area is 200 cm² at 6 keV. The mask pattern is based on a pseudo random array with 255 × 257 elements of 1 mm², 50% of which are transparent.     

Plasma effects of active ion beam injections in the ionosphere at rocket altitudes

Data from ARCS rocket ion beam injection experiments will be primarily discussed in this paper. There are three results from this series of active experiments that are of particular interest in space plasma physics. These are the transverse acceleration of ambient ions in the large beam volume, the scattering of beam ions near the release payload, and the possible acceleration of electrons very close to the plasma generator which produce intense high frequency waves. The ability of 100 ma ion beam injections into the upper E and F regions of the ionosphere to produce these phenomena appear to be related solely to the process by which the plasma release payload and the ion beam are neutralized. Since the electrons in the plasma release do not convect with the plasma ions, the neutralization of both the payload and beam must be accomplished by large field-aligned currents (milliamperes/square meter) which are very unstable to wave growth of various modes. Future work will concentrate on the wave production and wave-particle interactions that produce the plasma/energetic particle effects discussed in this paper and which have direct application to natural phenomena in the upper ionosphere and magnetosphere.