生物组织光学参数：优化散射系数（μ’s）的实时在位测定

利用了一套特殊设计光纤探头组成的稳定态光纤光谱仪测试生物组织的光学参数，从悬乳液(Intralpid)与模拟胶(Phantom)实验推导出优化散射系数(Reduced scattering coeffcient：μ’s)近红外谱测量经验公式。测量反射系数就可以计算出优化散射系数谱。计算结果用模拟胶作了验证，并对大鼠脑组织μ’s进行了实时在位测量，从而得到了一种μ’s在位测量的有效方法。     

Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms.

The space radiation environment is a mixed field consisting of different particles having different energies, including high charge and energy (HZE) ions. Conventional measurements of absorbed doses may not be sufficient to completely characterise the radiation field and perform reliable estimates of health risks. Biological dosimetry, based on the observation of specific radiation-induced endpoints (typically chromosome aberrations), can be a helpful approach in case of monitored exposure to space radiation or other mixed fields, as well as in case of accidental exposure. Furthermore, various ratios of aberrations (e.g. dicentric chromosomes to centric rings and complex exchanges to simple exchanges) have been suggested as possible fingerprints of radiation quality, although all of them have been subjected to some criticisms. In this context a mechanistic model and a Monte Carlo code for the simulation of chromosome aberration induction were developed. The model, able to provide dose-responses for different aberrations (e.g. dicentrics, rings, fragments, translocations, insertions and other complex exchanges), was further developed to assess the dependence of various ratios of aberrations on radiation quality. The predictions of the model were compared with available data, whose experimental conditions were faithfully reproduced. Particular attention was devoted to the scoring criteria adopted in different laboratories and to possible biases introduced by interphase death and mitotic delay. This latter aspect was investigated by taking into account both metaphase data and data obtained with Premature Chromosome Condensation (PCC).     

Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

The detection of energetic cometary and solar particles by the EPONA instrument on the Giotto mission

EPONA is an energetic particle detector system incorporating totally depleted silicon surface barrier layer detectors. Active and passive background shielding will be employed and, by applying various techniques, particles of different species, including electrons, protons, alpha particles and pick-up ions of cometary origin may be detected over a wide spectrum of energies extending from the tens of KeV into the MeV range.

The instrument can operate in two modes namely (a) in a cruise phase or storage mode and (b) in a real time mode. During the real time mode, observations at high spatial (octosectoring) and temporal (0.5s) resolution in the cometary environment permit studies to be made of accelerated particles at the bow shock and/or in the tail of the comet. In conjunction with magnetic field measurements on board Giotto, observations of energetic electrons and their anisotropies can determine whether the magnetic field lines in the cometary tail are open or closed. Further, the absorption of low energy solar particles in the cometary atmosphere can be measured and such data would provide an integral value of the pertaining gas and dust distribution. Solar particle background measurements during encounter may also be used to correct the measurements of other spacecraft borne instruments potentially vulnerable to such radiation.

Solar particle flux measurements, obtained during the cruise phase will, when combined with simultaneous observations made by other spacecraft at different heliographic longitudes, provide information concerning solar particle propagation in the corona and in interplanetary space.     

Charged-particle mutagenesis II. Mutagenic effects of high energy charged particles in normal human fibroblasts.

The biological effects of high LET charged particles are a subject of great concern with regard to the prediction of radiation risk in space. In this report, mutagenic effects of high LET charged particles are quantitatively measured using primary cultures of human skin fibroblasts, and the spectrum of induced mutations are analyzed. The LET of the charged particles ranged from 25 KeV/micrometer to 975 KeV/micrometer with particle energy (on the cells) between 94-603 MeV/u. The X-chromosome linked hypoxanthine guanine phosphoribosyl transferase (hprt) locus was used as the target gene. Exposure to these high LET charged particles resulted in exponential survival curves; whereas, mutation induction was fitted by a linear model. The Relative Biological Effect (RBE) for cell-killing ranged from 3.73 to 1.25, while that for mutant induction ranged from 5.74 to 0.48. Maximum RBE values were obtained at the LET of 150 keV/micrometer. The inactivation cross-section (alpha i) and the action cross-section for mutant induction (alpha m) ranged from 2.2 to 92.0 micrometer2 and 0.09 to 5.56 x 10(-3) micrometer2, respectively. The maximum values were obtained by 56Fe with an LET of 200 keV/micrometer. The mutagenicity (alpha m/alpha i) ranged from 2.05 to 7.99 x 10(-5) with the maximum value at 150 keV/micrometer. Furthermore, molecular analysis of mutants induced by charged particles indicates that higher LET beams are more likely to cause larger deletions in the hprt locus.     

Annual solar UV exposure and biological effective dose rates on the Martian surface.

The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux.     

Dayside reconnection during IMF northward: A possible foreshock effect

The northward and southward orientation of the interplanetary magnetic field (IMF) is usually considered as providing the external boundary conditions in the solar wind interaction with the Earth's magnetopause but it is the magnetic field in the magnetosheath that interacts with the Earth's magnetic field. In this paper, we consider the possibility that the wave activity in the foreshock region may affect the magnetic field orientation in the magnetosheath with time scales that might be geomagnetically effective. If magnetosheath magnetic field becomes disturbed on plasma streamlines which are connected to the quasi-parallel bow shock and foreshock, the magnetic field orientation on the inner magnetosheath may differ significantly from the undisturbed IMF. We present a model of dayside reconnection which may occur when the IMF northward and illustrate its effects on the erosion of the magnetopause.     

Solar UV irradiance variation during cycles 22 and 23

The Solar Ultraviolet Spectral Irradiance Monitor (SUSIM) aboard the Upper Atmosphere Research Satellite (UARS) has been measuring solar UV irradiances since October 1991, a period which includes the decline of solar cycle 22 followed by the rise of cycle 23. Daily solar measurements include scans over the wavelength range 115–410 nm at 1.1 nm resolution. As expected, the measured time series of UV irradiances exhibit strong periodicities in solar cycle and solar rotation. For all wavelengths, the UV irradiance time series are similar to that of the Mg II core-to-wing ratio. During solar cycle 22, the irradiance of the strong Ly- line varied by more than a factor of two. The peak-to-peak irradiance variation declined with increasing wavelength, reaching 10% just below the Al edge at 208 nm. Between the Al edge and 250 nm the variation was 6–7%. Above 250 nm, the variation declines further until none is observed above 290 nm. Preliminary results for the first portion of cycle 23 indicate that the far UV below the Al edge is rising at about the same rate as the Mg II index while the irradiances in the Ly- emission line and for wavelengths longer than the Al edge are rising more slowly — even after accounting for the lower level of activity of cycle 23.     

The effects of microgravity on induced mutation in Escherichia coli and Saccharomyces cerevisiae.

We examined whether microgravity influences the induced-mutation frequencies through in vivo experiments during space flight aboard the space shuttle Discovery (STS-91). We prepared dried samples of repair-deficient strains and parental strains of Escherichia (E.) coli and Saccharomyces (S.) cerevisiae given DNA damage treatment. After culture in space, we measured the induced-mutation frequencies and SOS-responses under microgravity. The experimental findings indicate that almost the same induced-mutation frequencies and SOS-responses of space samples were observed in both strains compared with the ground control samples. It is suggested that microgravity might not influence induced-mutation frequencies and SOS-responses at the stages of DNA replication and/or DNA repair. In addition, we developed a new experimental apparatus for space experiments to culture and freeze stocks of E. coli and S. cerevisiae cells.