Structural and functional changes in man accompanying the weightlessness in "Skylab" flights: a mathematical approach

The present paper reports a kinetic analysis of changes of some physiological parameters, obtained from international literature, after changes in gravitational environment. The overall phenomenology of the adaptation to weightlessness is characterized by a rapid process followed by a slow one. The two processes show half time values differing by about five times. Also in the case of readaptation to gravity, after recovery on the Earth, two well resolved processes, showing different half time values, are observed. It is of interest to notice that the rate of response to weightlessness is lower than that to gravity. Of course, the half time values observed depend on the different physiological parameters considered. In any case, the experimental data suggest a general trend of many adaptive changes, that may all be described by a simple mathematical model.     

Skylab experiment M-092: results of the first manned mission

Blood pressure at 30-sec intervals, heart rate, and percentage increase in leg volume continuously were recorded during a 25-min protocol in the M092 Inflight Lower Body Negative Pressure (LBNP) experiment carried out in the first manned Skylab mission. These data were collected during six tests on each crewman over a 5-month preflight period. The protocol consisted of a 5-min resting control period, 1 min at -8, 1 min at -16, 3 min at -30, 5 min at -40, and 5 min at -50 mm Hg LBNP. A 5-min recovery period followed. Inflight tests were performed at approximately 3-day intervals through the 28-day mission. Individual variations in cardiovascular responses to LBNP during the preflight period continued to be demonstrated in the inflight tests. Measurements of the calf indicated that a large volume of fluid was shifted out of the legs early in the flight and that a slower decrease in leg volume, presumably due to loss of muscle tissue, continued throughout the flight. Resting heart rates tended to be low early in the flight and to increase slightly as the flight progressed. Resting blood pressure varied but usually was characterized by slightly elevated systolic blood pressure, lower diastolic pressure, and higher pulse pressures than during preflight examinations. During LBNP inflight a much greater increase in leg volume occurred than in preflight tests. Large increases occurred even at the smallest levels of negative pressure, suggesting that the veins of the legs were relatively empty at the beginning of the LBNP. The greater volume of blood pooled in the legs was associated with greater increases of heart rate and diastolic pressure and larger falls of systolic and pulse pressure than seen in preflight tests. The LBNP protocol represented a greater stress inflight, and on three occasions it was necessary to stop the test early because of impending syncopal reactions. LBNP responses inflight appeared to predict the degree of postflight orthostatic intolerance. Postflight responses to LBNP during the first 48 hours were characterized by marked elevations of heart rate and instability of blood pressure. In addition, systolic and diastolic pressures were typically elevated considerably both at rest and also during stress. The time required for cardiovascular responses to return to preflight levels was much slower than in the case of Apollo crewmen.     

Mineral and nitrogen balance study: results of metabolic observations on Skylab II 28-day orbital mission

Prediction that the various stresses of flight, particularly weightlessness, would bring about significant derangements in the metabolism of the musculoskeletal system has been based on various observations of long-term immobilized or inactive bed rest. The only attempt at controlled measurement of metabolic changes in space prior to Skylab, a study during the 14-day Gemini VII flight, revealed rather modest losses of important elements. The three astronauts of Skylab II consumed a planned day-by-day, quite constant, dietary intake of major metabolic elements in mixed foods and beverages and provided virtually complete collections of excreta for 31 days preflight, during the 28 days inflight, and for 17 days postflight. Analyses showed that, in varying degree among the crewmen, urinary calcium increased gradually during flight in a pattern similar to that observed in bed-rest studies: the mean plateau peak of urinary calcium excretion in the latter part of flight was double preflight levels. Fecal calcium excretion did not change significantly, but calcium balance, owing to the urinary calcium rise, became either negative or less positive than in preflight measurement. Increased excretion and negative balance of nitrogen and phosphorus indicated appreciable loss of muscle tissue in all three crewmen. Significant losses also occurred inflight in potassium, sodium, and magnesium. Based on the similarity in pattern and degree between these observations and those in bed rest of the losses in calcium, phosphorus, and nitrogen, musculoskeletal integrity would not be threatened in space flights of up to at least 3 months. However, if similar changes occur, indicative of continuing losses of these elements, in the planned Skylab flights for considerably more than 28 days, concern for capable musculoskeletal function should be serious for flights of very many months' duration, and greater research attention will need to be given to development of protective counter-measures.     

Vibration and pulsation processes in feed systems of liquid rocket engines

Pulsation and vibration process in high speed centrifugal pumps and feed lines were investigated. Linear and quadratic relations between pulsation parameters and main factors were obtained. The investigation of turbopump low frequency oscillations showed that the intensity of pulsations is proportional to the pump specific linear size. Linear mathematic models with lumped parameters fairly well demonstrate the vibration influence on low frequency flow pressure vibrations in pumps and feed lines. It is shown that, in some conditions, the pressure oscillation amplitude distribution by the vibrating feed lines length may be characterized by the presence of a node in an intermediate section. A one-dimensional mathematic model based on wave equations proved to be suitable for calculations of vibration and pulsation parameters within a wide frequency range.     

Changes in organ perfusion and weight ratios in post-simulated microgravity recovery

Head-down tilt models have been used as ground-based simulations of microgravity. Our previous animal research has demonstrated that there are significant changes in fluid distribution within 2 h after placement in a 45 degrees head-down tilt (45HDT) position and these changes in fluid distribution were still present after 14 days of 45HDT. Consequently, we investigated changes in fluid distribution during recovery from 16 days of 45HDT. Changes in radioactive tracer distribution and organ/body weight ratio were examined in rats randomly assigned to a 45HDT or prone control group. The 45HDT rats were suspended for 16 days and then allowed to recover at the prone position 0, 77, 101, or 125 h post-suspension. Animals were injected with technetium-labeled diethylenetriamine pentaacetate (99mTcDTPA, MW=492 amu, physical half-life of 6.02 h) and then killed 30 min post-injection. Lungs, heart, liver, spleen, kidneys, and brain were harvested, weighed, and measured for radioactive counts. Statistical analyses included two-way analysis of variance (ANOVA) that compared 45HDT versus controls at the four experimental time points. The organ weight divided by the body weight ratio for the brain, heart, kidneys and liver in the 45HDT rats was significantly different than the control rats, regardless of time (treatment). There was no difference between the different time points (time). The average 99mTcDTPA count divided by the organ weight ratio values for the heart, liver, and spleen were significantly higher in the 45HDT group than the control group. The average counts for the heart and spleen were significantly higher at 77, 101, and 125 h than at time zero. We conclude that the major organs have different recovery patterns after 45HDT for 16 days in the rat.     

Future investigations onboard Soviet biosatellites of the Cosmos series.

Many rat experiments onboard Cosmos biosatellites have furnished information concerning the effects of weightlessness, artificial gravity, and ionizing radiation combined with weightlessness on structural and biochemical parameters of the animal body. The necessity to expand the scope of physiological investigations has led to the project of flight primate studies. It is planned to carry out the first primate experiments onboard the Cosmos biosatellite in 1982. At present investigations of weightlessness effects on the cardiovascular and vestibular systems, higher nervous activity, skeletal muscles and biorhythms of two rhesus monkeys are being developed and tested. It is also planned to conduct a study of weightlessness effects on embryogenesis of rats and bioenergetics of living systems onboard the same biosatellite. Further experiments onboard Cosmos biosatellites are planned.     

The cost benefits of the Spacelab system to scientific investigations and space applications

In the past, one of the major problems in performing scientific investigations in space has been the high cost of developing, integrating, and transporting scientific experiments into space. The limited resources of unmanned spacecraft, coupled with the requirements for completely automated operations, was another factor contributing to the high costs of scientific research in space. In previous space missions after developing, integrating and transporting costly experiments into space and obtaining successful data, the experiment facility and spacecraft have been lost forever, because they could not be returned to earth. The objective of this paper is to present how the utilization of the Spacelab System will result in cost benefits to the scientific community, and significantly reduce the cost of space operations from previous space programs.The following approach was used to quantify the cost benefits of using the Spacelab System to greatly reduce the operational costs of scientific research in space. An analysis was made of the series of activities required to combine individual scientific experiments into an integrated payload that is compatible with the Space Transportation System (STS). These activities, including Shuttle and Spacelab integration, communications and data processing, launch support requirements, and flight operations were analyzed to indicate how this new space system, when compared with previous space systems, will reduce the cost of space research. It will be shown that utilization of the Spacelab modular design, standard payload interfaces, optional Mission Dependent Equipment (MDE), and standard services, such as the Experiment Computer Operating System (ECOS), allow the user many more services than previous programs, at significantly lower costs. In addition, the missions will also be analyzed to relate their cost benefit contributions to space scientific research.The analytical tools that are being developed at MSFC in the form of computer programs that can rapidly analyze experiment to Spacelab interfaces will be discussed to show how these tools allow the Spacelab integrator to economically establish the payload compatibility of a Spacelab mission.The information used in this paper has been assimilated from the actual experience gained in integrating over 50 highly complex, scientific experiments that will fly on the Spacelab first and second missions. In addition, this paper described the work being done at the Marshall Space Flight Center (MSFC) to define the analytical integration tools and techniques required to economically and efficiently integrate a wide variety of Spacelab payloads and missions. The conclusions reached in this study are based on the actual experience gained at MSFC in its roles of Spacelab integration and mission managers for the first three Spacelab missions. The results of this paper will clearly show that the cost benefits of the Spacelab system will greatly reduce the costs and increase the opportunities for scientific investigation from space.     

Diuretic as a means for rapid adaptation to weightlessness

In a number of flights, cosmonauts and astronauts have experienced aggravation of their health status and general condition in the initial hours and days in a weightless environment. One of the trigger mechanisms for the onset of these unfavourable conditions at the start of space flight is a redistribution of body fluids and a blood shift towards the head. To ensure controlled hypohydration as a countermeasure to the deleterious effects of 0-g and to investigate the feasibility to control adaptation, six cosmonauts were administered lasix once a day during the first 3 days of a mission. All data of the experiment (correction test, questionnaire, hematocrit) were recorded on a special form in the logbook and transmitted to the control centre for processing. Results showed that the diuretic weakened the sensation of discomfort and improved the cosmonauts' general condition. Objective indices of the correction test indicate an increased work ability of cosmonauts. After hypohydration, circulating plasma volumes in the group were reduced by 6.8 + 1.0% on average.     

Choroidal responses in microgravity. (SLS-1, SLS-2 and hindlimb-suspension experiments)

Fluid and electrolyte shifts occuring during human spaceflight have been reported and investigated at the level of blood, cardio-vascular and renal responses. Very few data were available concerning the cerebral fluid and electrolyte adaptation to microgravity, even in animal models. It is the reason why we developed several studies focused on the effects of spaceflight (SLS-1 and SLS-2 programs, carried on NASA STS 40 and 56 missions, which were 9- and 14-day flights, respectively), on structural and functional features of choroid plexuses, organs which secrete 70–90 % of cerebrospinal fluid (CSF) and which are involved in brain homeostasis. Rats flown aboard space shuttles were sacrificed either in space (SLS-2 experiment, on flight day 13) or 4–8 hours after landing (SLS-1 and SLS-2 experiments). Quantitative autoradiography performed by microdensitometry and image analysis, showed that lateral and third ventricle choroid plexuses from rats flown for SLS-1 experiment demonstrated an increased number (about x 2) of binding sites to natriuretic peptides (which are known to be involved in mechanisms regulating CSF production). Using electron microscopy and immunocytochemistry, we studied the cellular response of choroid plexuses, which produce cerebrospinal fluid (CSF) in brain lateral, third and fourth ventricles. We demonstrated that spaceflight (SLS-2 experiment, inflight samples) induces changes in the choroidal cell structure (apical microvilli, kinocilia organization, vesicle accumulation) and protein distribution or expression (carbonic anhydrase II, water channels,…). These observations suggested a loss of choroidal cell polarity and a decrease in CSF secretion. Hindlimb-suspended rats displayed similar choroidal changes. All together, these results support the hypothesis of a modified CSF production in rats during long-term (9, 13 or 14 days) adaptations to microgravity.     

Precipitating Protons and Their Role in Ionization of the Polar Ionosphere

Spatial distributions of the electron density in the latitude range 60°–90° N were calculated on the basis of a physical model of the E and lower Fregions of the high-latitude ionosphere using statistical models of auroral proton and electron precipitation. It is shown that precipitating protons can play the key role in the ionization of the Eregion in the dusk and midnight sectors of the auroral oval. However, quantitative estimates of the contribution of protons to the ionization depend on the used statistical models of electron precipitation. Comparison of the electron density profiles calculated for two incoherent scatter radars, EISCAT (Tromsö) and ESR (Svalbard), for simultaneous precipitation of electrons and protons and for electron precipitation only show that the influence of protons is the most significant in the dusk sector over the EISCAT radar and in the midnight sector over the ESR radar. The results presented indicate the need to take protons into account when radar data are used to derive precipitating electron spectra.