Microorganisms metabolizing on clay grains in 3-km-deep Greenland basal ice

We have discovered > 10(8) microbial cells/cm3 attached to clay grains in the bottom 13 m of the GISP2 (Greenland Ice Sheet Project) ice core. Their concentration correlates with huge excesses of CO2 and CH4. We show that Fe-reducing bacteria produce most of the excess CO2 and methanogenic archaea produce the excess CH4. The number of attached cells per clay grain is proportional to grain perimeter rather than to area, which implies that nutrients are accessed at grain edges. We conclude that Fe-reducing microbes immobilized on clay surfaces metabolize via "shuttle" molecules that transport electrons to grain edges, where they reduce Fe(III) ions at edges to Fe(II) while organic acid ions are oxidized to CO2. Driven by the concentration gradient, electrons on Fe(II) ions at grain edges "hop" to Fe(III) ions inward in the same edges and oxidize them. The original Fe(III) ions can then attach new electrons from shuttle molecules at the edges. Our mechanism explains how Fe-reducers can reduce essentially all Fe(III) in clay minerals. We estimate that the Fe(III) in clay grains in the GISP2 silty ice can sustain Fe-reducing bacteria at the ambient temperature of -9 degrees C for approximately 10(6) years. F420 autofluorescence imaging shows that > 2.4% of the cells are methanogens, which account for the excess methane.     

Trajectories for missions of low-thrust spacecraft aimed at delivery of soil samples from main belt asteroids and Phobos

Trajectories of spacecraft with electro-jet low-thrust engines are studied for missions planning to deliver samples of matter from small bodies of the Solar System: asteroids Vesta and Fortuna, and Martian moon Phobos. Flight trajectories are analyzed for the mission to Phobos, the limits of optimization of payload spacecraft mass delivered to it are determined, and an estimate is given to losses in the payload mass when a low-thrust engine with constant outflow velocity is used. The model of an engine with ideally regulated low thrust is demonstrated to be convenient for calculations and analysis of flight trajectories of a low-thrust spacecraft.     

Communicating bioastronautics research to students, families and the nation

The National Space Biomedical Research Institute (NSBRI) is supporting the National Aeronautics and Space Administration's (NASA) education mission through a comprehensive Education and Public Outreach Program (EPOP) that communicates the excitement and significance of space biology to schools, families, and lay audiences. The EPOP is comprised of eight academic institutions: Baylor College of Medicine, Massachusetts Institute of Technology, Morehouse School of Medicine, Mount Sinai School of Medicine, Texas A&M University, University of Texas Medical Branch Galveston, Rice University, and the University of Washington. This paper describes the programs and products created by the EPOP to promote space life science education in schools and among the general public. To date, these activities have reached thousands of teachers and students around the US and have been rated very highly.     

Application of GFP technique for cytoskeleton visualization onboard the International Space Station

Cytoskeleton recently attracted wide attention of cell and molecular biologists due to its crucial role in gravity sensing and trunsduction. Most of cytoskeletal research is conducted by the means of immunohistochemical reactions, different modifications of which are beneficial for the ground-based experiments. But for the performance onboard the space vehicles, they represent quite complicated technique which requires time and special skills for astronauts. In addition, immunocytochemistry provides only static images of the cytoskeleton arrangement in fixed cells while its localization in living cells is needed for the better understanding of cytoskeletal function. In this connection, we propose a new approach for cytoskeletal visualization onboard the ISS, namely, application of green fluorescent protein (GFP) from Aequorea victoria, which has the unique properties as a marker for protein localization in vivo. The creation of chimerical protein-GFP gene constructs, obtaining the transformed plant cells possessed protein-GFP in their cytoskeletal composition will allow receiving a simple and efficient model for screening of the cytoskeleton functional status in microgravity.     

OSIRIS-REx Flight Dynamics and Navigation Design

OSIRIS-REx is the first NASA mission to return a sample of an asteroid to Earth. Navigation and flight dynamics for the mission to acquire and return a sample of asteroid 101955 Bennu establish many firsts for space exploration. These include relatively small orbital maneuvers that are precise to ～1 mm/s, close-up operations in a captured orbit about an asteroid that is small in size and mass, and planning and orbit phasing to revisit the same spot on Bennu in similar lighting conditions. After preliminary surveys and close approach flyovers of Bennu, the sample site will be scientifically characterized and selected. A robotic shock-absorbing arm with an attached sample collection head mounted on the main spacecraft bus acquires the sample, requiring navigation to Bennu’s surface. A touch-and-go sample acquisition maneuver will result in the retrieval of at least 60 grams of regolith, and up to several kilograms. The flight activity concludes with a return cruise to Earth and delivery of the sample return capsule (SRC) for landing and sample recovery at the Utah Test and Training Range (UTTR).     

Influence of Body Waves,Instrumentation Resonances,and Prior Assumptions on Rayleigh Wave Ellipticity Inversion for Shallow Structure at the InSight Landing Site

Based on an updated model of the regolith’s elastic properties, we simulate the ambient vibrations background wavefield recorded by InSight’s Seismic Experiment for Interior Structure (SEIS) on Mars to characterise the influence of the regolith and invert SEIS data for shallow subsurface structure. By approximately scaling the synthetics based on seismic signals of a terrestrial dust devil, we find that the high-frequency atmospheric background wavefield should be above the self-noise of SEIS’s SP sensors, even if the signals are not produced within 100–200 m of the station. We compare horizontal-to-vertical spectral ratios and Rayleigh wave ellipticity curves for a surface-wave based simulation on the one hand with synthetics explicitly considering body waves on the other hand and do not find any striking differences. Inverting the data, we find that the results are insensitive to assumptions on density. By contrast, assumptions on the velocity range in the upper-most layer have a strong influence on the results also at larger depth. Wrong assumptions can lead to results far from the true model in this case. Additional information on the general shape of the curve, i.e. single or dual peak, could help to mitigate this effect, even if it cannot directly be included into the inversion. We find that the ellipticity curves can provide stronger constraints on the minimum thickness and velocity of the second layer of the model than on the maximum values. We also consider the effect of instrumentation resonances caused by the lander flexible modes, solar panels, and the SEIS levelling system. Both the levelling system resonances and the lander flexible modes occur at significantly higher frequencies than the expected structural response, i.e. above 35 Hz and 20 Hz, respectively. While the lander and solar panel resonances might be too weak in amplitude to be recorded by SEIS, the levelling system resonances will show up clearly in horizontal spectra, the H/V and ellipticity curves. They are not removed by trying to extract only Rayleigh-wave dominated parts of the data. However, they can be distinguished from any subsurface response by their exceptionally low damping ratios of 1% or less as determined by random decrement analysis. The same applies to lander-generated signals observed in actual data from a Moon analogue experiment, so we expect this analysis will be useful in identifying instrumentation resonances in SEIS data.     

Influence Analysis of the Second Bypass Air Bleed Parameters on the Engine Performance

This paper considers double bypass aircraft engine with controlled air bleed to the second bypass duct. Its performance is evaluated by mathematical modeling techniques. The second bypass air bleed amount is determined to provide the best engine efficiency at various operating modes.