SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

Seismology of Prominence-Fine structures: Observations and Theory

Prominence seismology is a rapidly developing topic which seeks to infer the internal structure and properties of solar prominences from the study of its oscillations. Two-dimensional high-resolution observations suggest that filaments can be considered as made by small scale fibrils, having a cool region, stacked one after another in the vertical and horizontal directions. An extense observational background about oscillations in filaments has been gathered during the last 20 years and these observations point out that fibrils or groups of fibrils can oscillate independently. From the theoretical point of view, small amplitude oscillations in single and multifibril configurations have been studied as a first step to explain observational features.     

Recent Progress in Physics-Based Models of the Plasmasphere

We describe recent progress in physics-based models of the plasmasphere using the fluid and the kinetic approaches. Global modeling of the dynamics and influence of the plasmasphere is presented. Results from global plasmasphere simulations are used to understand and quantify (i) the electric potential pattern and evolution during geomagnetic storms, and (ii) the influence of the plasmasphere on the excitation of electromagnetic ion cyclotron (EMIC) waves and precipitation of energetic ions in the inner magnetosphere. The interactions of the plasmasphere with the ionosphere and the other regions of the magnetosphere are pointed out. We show the results of simulations for the formation of the plasmapause and discuss the influence of plasmaspheric wind and of ultra low frequency (ULF) waves for transport of plasmaspheric material. Theoretical models used to describe the electric field and plasma distribution in the plasmasphere are presented. Model predictions are compared to recent Cluster and Image observations, but also to results of earlier models and satellite observations.     

Water in Space: The Water World of ISO

In this review we present the main results obtained by the ISO satellite on the abundance and spatial distribution of water vapor in the direction of molecular clouds, evolved stars, galaxies, and in the bodies of our Solar System. We also discuss the modeling of H₂O and the difficulties found in the interpretation of the data, the need of collisional rates and the perspectives that future high angular and high spectral resolution observations of H₂O with the Herschel Space Observatory will open.     

A material experiment for small satellites to characterise the behaviour of carbon nanotubes in space – development and ground validation

Over the last years, Carbon Nanotubes (CNT) drew interdisciplinary attention. Regarding space technologies a variety of potential applications were proposed and investigated. However, no complex data on the behaviour and degradation process of carbon nanotubes under space environment exist. Therefore, it is necessary to investigate the performance of these new materials in space environment and to revaluate the application potential of CNTs in space technologies.Hence, CiREX (Carbon Nanotubes – Resistance Experiment) was developed as a part of a student project. It is a small and compact experiment, which is designed for CubeSat class space satellites. These are a class of nanosatellites with a standardized size and shape. The CiREX design, electrical measurements and the satellites interfaces will be discussed in detail. CiREX is the first in-situ space material experiment for CNTs.To evaluate the data obtained from CiREX, ground validation tests are mandatory. As part of an extensive test series the behaviour of CNTs under solar ultra violet light (UV) and vacuum ultraviolet light (VUV) was examined. Single-walled carbon nanotubes (SWNT), multi-walled carbon nanotubes (MWNT) and MWNT/resin composite (ME) were exposed to different light sources. After the exposure, the defect density was investigated with Raman spectroscopy. There is a clear indication that UV and VUV light can increase the defect density of untreated CNTs and influence the electrical behaviour.