The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.     

Airborne radar: its beginnings in World War II

Although not realized at the time, the defeat of the German Air Force in the summer of 1940 was one of the crucial battles of World War II. The narrow margin of victory can be ascribed to Britain's air-warning radar, Chain Home (CH), consisting of the 15-meter CH and the 1.5-meter CHL. The skillful use of this equipment by the Royal Air Force made daylight bombing unsustainable; the Luftwaffe then turned to night attacks, generally called "The Blitz." These were not effective in destroying military and industrial targets and depended on the hope of reducing the population's will to fight. Chain Home was of little use except to observe the attackers arrival, as it had almost no ability to follow the attackers as they proceeded inland. This eventuality was reckoned with before the outbreak of hostilities and had called for radars mounted in night fighters capable of guiding the pilot close enough to the target for him to open fire visually. The electronic techniques used at 1.5-meters were adapted to planes capable of carrying the radar and its operator. But there were three important design constraints: 1) the antennas had to be restricted to sizes that were practical for installation on aircraft, which for meter waves gave them low gain and large side lobes; 2) the set's maximum range was limited by the fighter's altitude as a result of the huge ground returns from the side lobes. British antiaircraft artillery, the stepchild of their arms, was too ineffective to drive the bombers to extreme altitudes; and 3) a minimum range had to be held until the flier could see his target, which strained the pulse techniques of the time.     

Automotive-grade MEMS sensors used for general aviation

The attitude heading reference system (AHRS) provides data for primary flight instruments, head-up displays, autopilots, and moving map navigation systems. Advances in solid-state MEMS rate sensors, coupled with Kalman filter algorithms designed to mitigate high drift rates, provide the basis for low-cost, high-performance AHRS for general aviation. This paper describes the performance of a low cost, miniaturized AHRS using automotive-grade MEMS sensors. The performance of the system is detailed. The implications for certification of this class of system and fault tolerance are discussed.     

Microdosimetric measurements of heavy ion tracks.

The biological effectiveness of radiations depends on the spatial pattern of ionizations and excitations produced by the charged particle tracks involved. Ionizations produced by both the primary ion and by energetic delta rays may contribute to the production of biologically relevant damage and to the concentration of damage which may effect the probability of repair. Although average energy concentration (dose) can be calculated using homogeneous track models, the energy is actually concentrated in small volumes containing segments of the ion and delta ray tracks. These local concentrations are studied experimentally using low pressure proportional counters, and theoretically, using Monte Carlo methods. Small volumes near an ion track may be traversed by a delta ray. If they are, the energy deposited will be similar to that produced by a single electron track in a low-energy x-ray irradiation. The probability of a delta ray interaction occurring decreases with the square of the radial distance from the track. The average energy deposited is the product of this probability and the energy deposited in an interaction. Average energy deposited calculated from measured interaction probability is in good agreement with the results of homogeneous track models.     

An experiment with an S-band radar using pulse compression andrange sidelobe suppression for meteorological measurements

A major technology barrier to the application of pulse compression for the meteorological functions required by a next generation ATC radar is range/time sidelobes which mask and corrupt observations of weak phenomena occurring near areas of strong extended meteorological scatterers. Techniques for suppressing range sidelobes are well known but without prior knowledge of the scattering medium's velocity distribution their performance degrades rapidly in the presence of Doppler. Recent investigations have presented a “doppler tolerant” range sidelobe suppression technique. The thrust of the work described herein is the extension of previous simulations to actual transmitted dispersed/coded waveforms using the S-band surveillance radar located at Rome Laboratory Surveillance Facility. The objectives of the experiment are: 1) to extend the verification of the simulation of the Doppler tolerant technique; and 2) to demonstrate that the radar transmitter, waveform generator, and receiver imperfections do not significantly degrade resolution, performance or reliability of meteorological spectral moment estimates     

Automated video protection, monitoring & detection

The latest generation of computer vision technology is revolutionizing concepts, applications, and products in video surveillance and CCTV. This is of prime relevance to security for large outdoor facilities such as commercial airfields, refineries, power plants, and office/industrial campuses. Most airfields, for example, have open (unfenced) perimeters, high volume heterogeneous traffic, are easily accessed on foot or by water, and exist in areas where regulations providing a safety buffer are difficult to legislate or enforce. And all airfields require 24/7 outdoor monitoring - snow, fog, rain, or shine. Likewise, most high-value facilities appealing to criminals and terrorists are in close proximity to public areas (roads, residences, city, etc.). The appeal of automated real-time surveillance is obvious $maximizing efficiency and effectiveness of security personnel and resources while increasing the probability of preventing a serious security breach. Computer vision based solutions have the potential for very discriminating detection and very low false alarms. The bottom line is that applied computer vision has the potential for the greatest return on investment (ROI), both short-term and long-term.     

Differential flank growth.

With the mathematical basis for the precise analysis of developmental processes in plants, the patterns of growth in phototropic and gravitropic responses have become better understood. A detailed temporal and spatial quantification of a growth process is an important tool for evaluating hypotheses about the underlying physiological mechanisms. Studies of growth rates and curvature show that the original Cholodny-Went hypothesis cannot explain the complex growth patterns during tropic responses of shoots and roots. In addition, regulating factors other than the lateral redistribution of hormones must be taken into account. Electrophysiological studies on roots led to a modification of the Cholodny-Went hypothesis in that redistributions of bioelectrical activities are observed.     

Field formation around negatively biased solar arrays in the LEO-plasma

Heavy emission caused by impacting plasma ions results in a fast discharging effect of the initially large surface potentials on the dielectric solar cells. This eventually counteracts the energization process of the plasma ions to the cover glasses and leaves no significant electric fields. Thus, with an existing thermal plasma, electrons are again able to reach dielectric surfaces. Strong localized electric fields of the order of several 10 kV/cm form near the interconnector-cover glass interface.