Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms.

The space radiation environment is a mixed field consisting of different particles having different energies, including high charge and energy (HZE) ions. Conventional measurements of absorbed doses may not be sufficient to completely characterise the radiation field and perform reliable estimates of health risks. Biological dosimetry, based on the observation of specific radiation-induced endpoints (typically chromosome aberrations), can be a helpful approach in case of monitored exposure to space radiation or other mixed fields, as well as in case of accidental exposure. Furthermore, various ratios of aberrations (e.g. dicentric chromosomes to centric rings and complex exchanges to simple exchanges) have been suggested as possible fingerprints of radiation quality, although all of them have been subjected to some criticisms. In this context a mechanistic model and a Monte Carlo code for the simulation of chromosome aberration induction were developed. The model, able to provide dose-responses for different aberrations (e.g. dicentrics, rings, fragments, translocations, insertions and other complex exchanges), was further developed to assess the dependence of various ratios of aberrations on radiation quality. The predictions of the model were compared with available data, whose experimental conditions were faithfully reproduced. Particular attention was devoted to the scoring criteria adopted in different laboratories and to possible biases introduced by interphase death and mitotic delay. This latter aspect was investigated by taking into account both metaphase data and data obtained with Premature Chromosome Condensation (PCC).     

A new spectral index to detect Poaceae grass abundance in Mongolian grasslands

The objectives of the present study were to develop a new index based on remotely-sensed data for detecting the abundance of grasses in the family Poaceae, which has a high palatability for livestock in Mongolia, and to map the distribution of these grasses in the semi-arid Mongolian steppes. We measured ground-based spectral reflectance of pure plant leaves – including Poaceae grasses – and soils, as well as in-situ in the Mongolian grasslands. The hyper-spectral data, taken by a spectroradiometer, were converted into four multi-spectral bands (i.e., blue, green, red, and NIR) to simulate satellite-based imagery data. In order to magnify the characteristics of the spectral signal of Poaceae, NGBDI (Normalized Green-Blue Difference Index), NGRDI (Normalized Green-Red Difference Index), NDVI (Normalized Difference Vegetation Index), NNBDI (Normalized NIR-Blue Difference Index) were calculated from the four multi-spectral reflectance values. Poaceae Abundance Index (PAI) was derived by combining these four normalized difference indices. PAI was found out to be a good indicator to discriminate Poaceae grass from the other plant spectral data.     

A point-wise least squares spectral analysis (LSSA) of the Caspian Sea level fluctuations,using TOPEX/Poseidon and Jason-1 observations

The Caspian Sea has displayed considerable fluctuations in its water level during the past century. Knowledge of such fluctuation is vital for understanding the local hydrological cycles, climate of the region, and construction activities within the sea and along its shorelines. This study established a point-wise satellite altimetry approach to monitor the fluctuations of the Caspian Sea using a complete dataset of TOPEX/Poseidon for the period 1993 to the middle of 2002, and its follow-on Jason-1 for the period 2002 to August 2009. Therefore, 280 virtual time-series were constructed to monitor the fluctuations. The least squares spectral analysis (LSSA) method is, then employed to find the most significant frequencies of the time-series, while the statistical method of principle component analysis (PCA) is applied to extract the dominant variability of level variations. The study also used the observations of TOPEX/Poseidon and Jason-1 over the Volga River along with 5 years of Volga’s water discharge to study its influence on the Caspian Sea level changes. The LSSA results indicate that the lunar semidiurnal (M2) and the Sun semidiurnal (S2) frequencies are the main tidal frequencies of the Caspian Sea with the mean amplitude of 4.2 and 2.8 cm, respectively. A statistically significant long-term frequency (12.5-years period) is also found from altimetry and tide gauge observations. A phase lag, related to the inter-annual frequencies of the Volga River was detected from the point-wise time-series showing level propagation from the northwest to the southeast of the sea. The cross-correlation between the power spectrum of Volga and that of the northern-most, middle, and southern-most points within the Caspian Sea were respectively 0.63, 0.51 and 0.4 of zero-lag correlation, corroborating the influence of the Volga River. The result of PCA also shows that different parts of the Caspian Sea exhibit different amplitudes of level variations, indicating that the point-wise approach, when employing all available satellite measurements could be a suitable method for a preliminary monitoring of this inland water resource as it gives accurate local fluctuations.     

Study of GNSS-measured ionospheric total electron content variations generated by powerful HF-heating

The purpose of this work is to report the experimental evidences for the influence of perturbations in the electron density in the dayside mid-latitude ionosphere, that are caused by high-frequency heating of the F2 layer, on the GNSS signals. The experiments were carried out at the Sura heater (Radio Physical Research Institute, N. Novgorod). During the sessions of ionospheric heating with different time modulations of the radiated power the rays linking the navigational satellites with the ground receiver intersected the heated region. Variations in the total electron content (TEC) were studied; these variations are proportional to the reduced phases of navigational signals. It is shown that with the square-wave modulation of the radiated power (with periods of 1, 6, 10 and 15 min), perturbations with periods of the main modulation of heating and its harmonics appear in the spectrum of TEC variations. Examples are presented of identification of the heating-induced variations in TEC, including determination of the amplitudes and time characteristics of these variations.     

Study of maximum electron density NmF2 at Karachi and Islamabad during solar minimum (1996) and solar maximum (2000) and its comparison with IRI

The monthly hourly medians of maximum electron density, N_mF₂, at two Pakistani ionospheric stations, Karachi and Islamabad, have been determined for solar minimum (1996) and solar maximum (2000) and compared with IRI predictions using the URSI coefficients. At night and pre-noon period the N_mF₂ values at both stations are almost equal during the 2 years. However, at post-noon the values at Karachi are considerably larger than those at Islamabad due to the equatorial or geomagnetic anomaly. Karachi (geomag. coord. 16.44°N, 139.08°E) lies near the region of the equatorial anomaly (+20 and −20 geomagnetic latitude), so most of the N_mF₂ values at Karachi are larger than those at Islamabad (geomag. coord. 24.46°N, 145.67°E). The maximum monthly values of N_mF₂ show a semi-annual variation at Karachi and Islamabad both during 1996 and 2000 as predicted by IRI.     

Airglow in the Visible and Infrared Spectral Ranges of the Disturbed Upper Atmosphere under Conditions of High-Velocity Plasma Jet Injection: I. Experimental data

The measurements of infrared emission from an artificial structure, which was generated during the Fluxus experiment with plasma jet injection into the atmosphere, are obtained and discussed for the first time. Additional experimental data on the airglow in the visible spectral band of the disturbed region of the atmosphere are presented. A generalized analysis of the data is given.     

Blood and small intestine cell kinetics under radiation exposures: Mathematical modeling

Mathematical models which describe the dynamics of two vital body systems (hematopoiesis and small intestinal epithelium) in mammals exposed to acute and chronic radiation are developed. These models, based on conventional biological theories, are implemented as systems of nonlinear differential equations. Their variables and constant parameters have clear biological meaning, that provides successful identification and verification of the models in hand. It is shown that the predictions of the models qualitatively and quantitatively agree with the respective experimental data for small laboratory animals (mice, rats) exposed to acute/chronic irradiation in wide ranges of doses and dose rates. The explanation of a number of radiobiological effects, including those of the low-level long-term exposures, is proposed proceeding from the modeling results. All this bears witness to the validity of employment of the developed models, after a proper identification, in investigation and prediction of radiation effects on the hematopoietic and small intestinal epithelium systems in various mammalian species, including humans. In particular, the models can be used for estimating effects of irradiation on astronauts in the long-term space missions, such as Lunar colonies and Mars voyages.     

Experimental Aspects of Mid-Frequency Pulsations (f≈10–100 mHz) in the Southern Polar Cap

We review the results obtained in the frequency range of Pc3 (22-100 mHz) and Pc4 (7-22 mHz) pulsations at Italian Antarctic stations in the southern polar cap (“Mario Zucchelli”, at Terra Nova Bay, TNB, 80^˚.S; “Concordia”, the Italian/French base at Dome C, DMC, 89^˚.S). The absence of a midnight enhancement in the pulsation power suggests a negligible substorm influence at extreme latitudes, while the sharp noon enhancement, which appears only at TNB, is determined by the closer proximity of the station to cusp related phenomena. The relationship between the frequency of the band-limited signals and the interplanetary magnetic field strength, the cone angle influence, and the higher correlation of the Pc3 power with the solar wind speed in the morning hours suggest a global scenario in which upstream waves would be mainly responsible for the mid-frequency activity in the polar cap. However, the polarization pattern is odd with respect to the predictions for tailward propagating modes.     

The Solar and Cosmic-Ray Synodic Periodicity (1969–1998)

The synodic recurrence of the Mt. Wilson plage index (MPSI) and the Calgary cosmic ray (CR) intensity is investigated, using the wavelet power spectra in the range of 18–38 days, during the last three solar cycles. The unique temporal coincidence between the quasi–synodic MPSI and the CR periods is detected in 1978–1982 (the 21st solar cycle). In the 22nd cycle there is a very strong MPSI synodic recurrence, from 1989.5 to 1990.5, but it is absent in the CR data. In 1992.5–1993.5 the MPSI and CR recurrence phenomenon is in good accordance with the solar wind speed and cosmic ray modulation as measured during the first Ulysses passage around the Sun. The Gnevyshev gap is present in the 27-day recurrence of CR, in agreement with Kudela et al. (1999). This revised version was published online in August 2006 with corrections to the Cover Date.     

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms.

The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Apr, Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E. coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E. coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.