Preparation of the Biochip experiment on the EXPOSE-R2 mission outside the International Space Station

Biochips might be suited for planetary exploration. Indeed, they present great potential for the search for biomarkers – molecules that are the sign of past or present life in space – thanks to their size (miniaturized devices) and sensitivity. Their detection principle is based on the recognition of a target molecule by affinity receptors fixed on a solid surface. Consequently, one of the main concerns when developing such a system is the behavior of the biological receptors in a space environment. In this paper, we describe the preparation of an experiment planned to be part of the EXPOSE-R2 mission, which will be conducted on the EXPOSE-R facility, outside the International Space Station (ISS), in order to study the resistance of biochip models to space constraints (especially cosmic radiation and thermal cycling). This experiment overcomes the limits of ground tests which do not reproduce exactly the space parameters. Indeed, contrary to ground experiments where constraints are applied individually and in a limited time, the biochip models on the ISS will be exposed to cumulated constraints during several months. Finally, this ISS experiment is a necessary step towards planetary exploration as it will help assessing whether a biochip can be used for future exploration missions.     

Semi-analytical investigations of high area-to-mass ratio geosynchronous space debris including Earth’s shadowing effects

This paper investigates the long-term perturbations of the orbits of geosynchronous space debris influenced by direct radiation pressure including the Earth’s shadowing effects. For this purpose, we propose an extension of our homemade semi-analytical theory [Valk, S., Lemaître, A., Deleflie, F. Semi-analytical theory of mean orbital motion for geosynchronous space debris under gravitational influence. Adv. Space Res., submitted for publication], based on the method developed by Aksnes [Aksnes, K. Short-period and long-period perturbations of a spherical satellite due to direct solar radiation. Celest. Mech. Dyn. Astron. 13, 89–104, 1976] and generalized into a more convenient non-singular formalism. The perturbations accounting for the direct radiation pressure with the Earth’s shadow are computed on a revolution-by-revolution basis, retaining the original osculating Hamiltonian disturbing function. In this framework, we compute the non-singular mean longitude at shadow entry and shadow exit at every orbital revolution in opposition to classical approaches where the singular eccentric anomalies at shadow entry and shadow exit are computed. This new algorithm is developed using non-singular variables. Consequently, it is particularly suitable for both near-circular and near-equatorial orbits as well as orbits which transit periodically around null eccentricities and null inclinations.The algorithm is tested by means of numerical integrations of the equations, averaged over the short periods, including radiation pressure, J₂, the combined Moon and Sun third body attraction as well as the long-term effects of the 1:1 resonance occurring for geosynchronous objects. As an extension of [Valk, S., Lemaître, A., Anselmo, L. Analytical and semi-analytical investigations of geosynchronous space debris with high area-to-mass ratios influenced by solar radiation pressure. Adv. Space Res., doi:10.1016/j.asr.2007.10.025, 2007b], we especially apply our analysis to space debris with area-to-mass as high as 20 m²/kg. This paper provides numerical and semi-analytical investigations leading to a deep understanding of the long-term evolution of the semi-major axis. Finally, these semi-analytical investigations are compared with accurate numerical integrations of the osculating equations of motion over time scales as high as 25 years.     

High resolution science with high redshift galaxies

We summarize the high-resolution science that has been done on high redshift galaxies with Adaptive Optics (AO) on the world’s largest ground-based facilities and with the Hubble Space Telescope (HST). These facilities complement each other. Ground-based AO provides better light gathering power and in principle better resolution than HST, giving it the edge in high spatial resolution imaging and high resolution spectroscopy. HST produces higher quality, more stable PSF’s over larger field-of-views in a much darker sky-background than ground-based AO, and yields deeper wide-field images and low-resolution spectra than the ground. Faint galaxies have steadily decreasing sizes at fainter fluxes and higher redshifts, reflecting the hierarchical formation of galaxies over cosmic time. HST has imaged this process in great structural detail to z  6, and ground-based AO and spectroscopy has provided measurements of their masses and other physical properties with cosmic time. Last, we review how the 6.5 m James Webb Space Telescope (JWST) will measure First Light, reionization, and galaxy assembly in the near–mid-IR after 2013.     

Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

Aerosols and clouds in the upper troposphere–lower stratosphere region detected by GOMOS and ACE: Intercomparison and analysis of the years 2004 and 2005

Satellite-based limb occultation measurements are well suited for the detection and mapping of polar stratospheric clouds (PSCs) and cirrus clouds. PSCs are of fundamental importance for the formation of the Antarctic ozone hole that occurs every year since the early 1980s in Southern Hemisphere spring. Despite progress in the observation, modeling and understanding of PSCs in recent years, there are still important questions which remain to be resolved, e.g. PSC microphysics, composition, formation mechanisms and long-term changes in occurrence. In addition, it has recently become clear that cirrus clouds significantly affect the global energy balance and climate, due to their influence on atmospheric thermal structure.     

Remote upgrading of a space-borne instrument

The main purposes of experiment “Obstanovka” (“Environment” in Russian) consisting of several instruments are to measure a set of electromagnetic and plasma phenomena characterizing the space weather conditions, and to evaluate how such a big and highly energy consuming body as the International Space Station disturbs the surrounding plasma, and how the station itself is charged due to the operation of so many instruments, solar batteries, life supporting devices, etc. Two identical Langmuir electrostatic probes are included in the experiment “Obstanovka”. In this paper the Langmuir probes for “Obstanovka” experiment are described, including the choice of geometry (spherical or cylindrical), a more reliable method for the sweep voltage generation, an adaptive algorithm for the probe’s operation. Special attention is paid to the possibility for remote upgrading of the instrument from the ground using the standard communication channels.