Biodosimetry of heavyions by interphase chromosome painting

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoingradiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2–3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchanges was much higher for patients treated with carbon ions than X-ray.     

The APT on the Polar Orbiting Weather Satellites

The receipt of the Pioneer Award has given me a chance to look back over my professional life and the opportunity to take stock of how I helped shape a small part of the world. While I hope this process entertains my contemporaries, more importantly, I hope it stimulates those that are engaged in actively shaping the present. To describe the need for automatic picture transmission (APT), I must retrace the historical development of meteorological satellites. The idea for weather observations from a satellite originated with a small group of meteorologists at the U.S. Army Signal Corps Research and Development Lab. at Ft. Monmouth, N.J., and resulted in the design of Vanguard II. The Tiros and TOS series of satellites, and the design of Nimbus, followed soon thereafter. However, a faster picture dissemination than was available at that time was needed, and it was this necessity that sparked the development of APT. Nimbus was originally intended to be an operational system, but the advent of simpler, less costly stabilization systems made the Tiros evolution the clear winner. The geosynchronous weather satellites started nearly a decade later and evolved from the NASA Application Technology Satellite (ATS) series. All three systems, existing polar orbiting weather satellites, APT, and geosynchronous weather satellites, have changed meteorology and the reliability of weather forecasting profoundly.     

Simultaneous measurement of multiple radiation-induced protein expression profiles using the Luminex(TM) system.

Space flight results in the exposure of astronauts to a mixed field of radiation composed of energetic particles of varying energies, and biological indicators of space radiation exposure provides a better understanding of the associated long-term health risks. Current methods of biodosimetry have employed the use of cytogenetic analysis for biodosimetry, and more recently the advent of technological progression has led to advanced research in the use of genomic and proteomic expression profiling to simultaneously assess biomarkers of radiation exposure. We describe here the technical advantages of the Luminex(TM) 100 system relative to traditional methods and its potential as a tool to simultaneously profile multiple proteins induced by ionizing radiation. The development of such a bioassay would provide more relevant post-translational dynamics of stress response and will impart important implications in the advancement of space and other radiation contact monitoring.     

Track segment association, fine-step IMM and initialization with Doppler for improved track performance

In this work we present a new track segment association technique to improve track continuity in large-scale target tracking problems where track breakages are common. A representative airborne early warning (AEW) system scenario, which is a challenging environment due to highly maneuvering targets, close target formations, large measurement errors, long sampling intervals, and low detection probabilities, provides the motivation for the new technique. Previously, a tracker using the interacting multiple model (IMM) estimator combined with an assignment algorithm was shown to be more reliable than a conventional Kalman filter based approach in tracking similar targets but it still yielded track breakages due to the difficult environment. In order to combine the broken track segments and improve track continuity, a new track segment association algorithm using a discrete optimization approach is presented. Simulation results show that track segment association yields significant improvements in mean track life as well as in position, speed, and course rms errors. Also presented is a modified one-point initialization technique with range rate measurements, which are typically ignored by other initialization techniques, and a fine-step IMM estimator, which improves performance in the presence of long revisit intervals. Another aspect that is investigated is the benefit of "deep" (multiframe or N-dimensional, with N > 2) association, which is shown to yield significant benefit in reducing the number of false tracks.     

Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Observations in the South Atlantic geomagnetic anomaly with intercosmos-Bulgaria-1300 during a geomagnetic storm

The region of South Atlantic Geomagnetic Anomaly (SAGA) was investigated by the Intercosmos-Bulgaria-1300 satellite, launched on August 7, 1981. On the basis of data obtained from 15 orbits during increased geomagnetic activity in August 1981, a map of the Anomaly was elaborated. Two centres of activity were identified. By means of the EMO-5 electrophotometer on board the Intercosmos-Bulgaria-1300 satellite, the atmosphere glow in lines λ 5577 Å, λ 6300 Å and λ 4278 Å was studied.     

On the structure and dynamics of the thermosphere

Thermospheric temperature, composition and wind measurements from the Dynamics Explorer satellite (DE-2) are interpreted using a three dimensional, multiconstituent spectral model. The analysis accounts for tides driven by the absorbed solar radiation as well as energy and momentum coupling involving the magnetosphere and lower atmosphere. We discuss phenomena associated with the annual tide, polar circulation, magnetic storms and substorms.     

The biological clock of Neurospora in a microgravity environment.

The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s). An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of flight, the tubes were exposed to light while their growth fronts were marked. Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. These results from the last 86 hours of flight demonstrated that the rhythm can persist in space. Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch. To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation. Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.     

History of monopulse radar in the USSR

Monopulse radars have played an important role in air and missile defense systems since the development of the monopulse technique in the late 1940s. This paper outlines the application of monopulse radars in Russian defense systems, starting with the Moscow ABM system and continuing in instrumentation and air defense radars now widely deployed in Russia and elsewhere     

In situ measurements to determine if Phobos is layered

The complex system of linear features on Phobos—the inner moon of Mars—found by the Viking Orbiters in 1976, can be classified morphologically into three types according to their appearance as well as their geometrical distribution on the surface. One kind of grooves appears to form arcs of small circles normal to the Phobos-Mars direction. We propose that these grooves are the surface manifestation of layering within Phobos[4–6] which could have been formed when Phobos was a part of a much larger and geologically active parent planet.Here we suggest some measurements to observe whether Phobos has a layered structure or not. The suggestion contains remote sensing measurements (particularly determination of the magnetic field by magnetometer and by electron reflection method) by a spacecraft orbiting around Mars as well as surface measurements (active seismic reflection experiment) by a landing module on Phobos.