On the levels of enzymatic substrate specificity: implications for the early evolution of metabolic pathways.

The most frequently invoked explanation for the origin of metabolic pathways is the retrograde evolution hypothesis. In contrast, according to the so-called "patchwork" theory, metabolism evolved by the recruitment of relatively inefficient small enzymes of broad specificity that could react with a wide range of chemically related substrates. In this paper it is argued that both sequence comparisons and experimental results on enzyme substrate specificity support the patchwork assembly theory. The available evidence supports previous suggestions that gene duplication events followed by a gradual neoDarwinian accumulation of mutations and other minute genetic changes lead to the narrowing and modification of enzyme function in at least some primordial metabolic pathways.     

Lunar habitat concept employing the space shuttle external tank

The space shuttle external tank, which consists of a liquid oxygen tank, an intertank structure, and a liquid hydrogen tank, is an expendable structure used for approximately 8.5 min during each launch. A concept for outfitting the liquid oxygen tank-intertank unit for a 12-person lunar habitat is described. The concept utilizes existing structures and openings for both man and equipment access without compromising the structural integrity of the tank. Living quarters, instrumentation, environmental control and life support, thermal control, and propulsion systems are installed at Space Station Freedom. The unmanned habitat is then transported to low lunar orbit and autonomously soft landed on the lunar surface. Design studies indicate that this concept is feasible by the year 2000 with concurrent development of a space transfer vehicle and manned cargo lander for crew changeover and resupply.     

Radar Cross Section of Ships

A laboratory method to determine the magnitude and position of radar reflection sources on complex targets is described. In addition the method provides a way to measure the modification of the radar cross section (RCS) due to multipath. The method has application in modeling RCS for radar and electronic countermeasure (ECM) system performance analysis and in the study of the extent to which the signature of the target could be altered. The equipment described, termed MACROSCOPE, was developed for RCS studies by the U.S. Army and is described in limited distribution bution literature. The application to marine targets is new with this paper, as is the technique of measuring the RCS of parts of the target and analytically combining them to represent the whole. An illustration of the need for this type of laboratory equipment was illustrated by the extensive search for full scale data which could be compared to scale model data to validate the technique.     

The Crab pulsar seen with AquEYE at Asiago Cima Ekar observatory

We are developing fast photon-counter instruments to study the rapid variability of astrophysical sources by time tagging photon arrival times with unprecedented accuracy, making use of a Rubidium clock and GPS receiver. The first realization of such optical photon-counters, dubbed AquEYE (the Asiago Quantum Eye), was mounted in 2008 at the 182 cm Copernicus Observatory in Asiago. AquEYE observed the Crab pulsar several times and collected data of extraordinary quality that allowed us to perform accurate optical timing of the Crab pulsar and to study the pulse shape stability on a timescale from days to years with an excellent definition. Our results reinforce the evidence for decadal stability of the inclination angle between the spin and magnetic axis of the Crab pulsar. Future realizations of our instrument will make use of the Galileo Global Navigation Satellite System (GNSS) time signal.     

Characterization of an acoustic liner by means of Laser Doppler Velocimetry in a subsonic flow

This paper describes a new experimental approach to acoustic liner characterization in the presence of a grazing flow. The traditional methods of measurement use microphones to determine liner impedance. The in situ method in particular requires the simultaneous use of two microphones. The first is mounted flush with the surface of the liner grazed by the flow and the second is flush-mounted to the rear face of the liner. However, this method is invasive and assumes the reaction of the liner to be independent of the incidence of the waves (locally-reacting liner). The approach suggested here is radically different since Laser Doppler Velocimetry (LDV) is used to measure the acoustic perturbation of velocity, or acoustic velocity. This latter allows us to determine the acoustic displacement, which is the key parameter in Galbrun's linear theory for assessing the perturbation of pressure and the field of active intensity. The wall impedance and the propagation paths of acoustic energy in the presence of the liner may be deduced without any assumption and non-invasively. This approach was applied for characterizing a resistive liner in a test bench specially designed for aeroacoustic measurements, with a 2D LDV system. The flow was turbulent and the measured nominal Mach number was 0.13. The impedance and field of active intensity were then obtained. A comparison was carried out between the new approach and the in situ method using microphones. According to previous theoretical works in the literature and the presented test results, one has to be cautious about the definition of the impedance when performing in-flow acoustic measurements.     

The human cardiovascular system during space flight

Purpose of the work is to analyze and to summarize the data of investigations into human hemodynamics performed over 20 years aboard orbital stations Salyut-7 and Mir with participation of 26 cosmonauts on space flights (SF) from 8 to 438 days in duration. The ultrasonic techniques and occlusive plethysmography demonstrated dynamics of changes in the cardiovascular system during SF of various durations. The parameters of general hemodynamics, the pumping function of the heart and arterial circulation in the brain remained stable in all the space flights; however, there were alterations in peripheral circulation associated with blood redistribution and hypovolemie in microgravity. The anti-gravity distribution of the vascular tone decayed gradually as unneeded. The most considerable changes were observed in leg vessels, equally in arteries (decrease in resistance) and veins (increase in maximum capacity). The lower body negative pressure test (LBNP) revealed deterioration of the gravity-dependent reactions that changed for the worse as SF duration extended. The cardiovascular deconditioning showed itself as loss of descent acceleration tolerance and orthostatic instability in the postflight period.     

Microperturbations on the <Emphasis Type=Italic>International Space Station</Emphasis> during physical exercises of the crew

The results of a preliminary analysis of microperturbations on the International Space Station during physical exercises of the crew are presented. The goal of this paper is to identify the parameters of perturbations when physical exercises are performed. The results of measurements by sensors of microaccelerations of both Russian and American segments during physical exercises in the service module of the Russian segment are analyzed.     

Structure of the earth’s magnetosheath at small fluctuations in the interplanetary magnetic field direction

Spatial structure of the magnetosheath of the Earth was studied under the conditions when no sharp (more than 40° during 5 min) changes in the interplanetary magnetic field direction were observed. On the basis of 24 flights of the Interball-1 satellite through the magnetosheath, it is found that three regions differing from each other by parameters of the field and plasma can be observed in the magnetosheath under the above-indicated conditions. These regions also differ from the solar wind region before front of the Earth’s magnetospheric bow shock. Empirical distributions of parameters were studied in each region. Taking into account the influence of the interplanetary magnetic field direction on the processes in the magnetosheath, the cases of quasi-perpendicular and quasi-parallel shock waves were considered separately. The study showed that the distribution of parameters in the selected regions (in the solar wind before front of the bow shock, in the magnetosheath behind the bow shock (post-shock), in the region of the magnetosheath with minimal fluctuations in the field, and in the inner magnetosheath) differ from each other at any interplanetary magnetic field direction.     

Analytic expansions of luni-solar gravity perturbations along rotating axes for trajectory optimization—Part 2: The multipliers system and simulations

An efficient self-contained trajectory optimization software is generated by making use of de Pontécoulant's analytic lunar theory removing the need for an outside third body ephemeris program to compute the lunar and solar position vectors at each integration step. The accelerations being further resolved along the rotating Euler–Hill frame after expansion to third order in the spacecraft radial distance, the adjoint differential equations are derived in a direct manner complementing the generation of the dynamic system of equations for full compatibility. Because the variation of parameters equations are cast in terms of the nonsingular equinoctial elements with the perturbation accelerations resolved in their analytic form along the rotating axes, the adjoint equations are also derived in the same manner providing a highly efficient and accurate system of equations for rapid computations in conjunction with Aerospace Corporation's NLP2 nonlinear programming codes to search for the initial values of the multipliers that steer the spacecraft towards its target orbit in minimum time. Numerical simulations show that the solutions obtained by the analysis developed in this paper are essentially identical to the more indirect approach based on the use of inertial accelerations obtained from a separate ephemeris generator and subsequent conversions to the thrust frame and equinoctial system.