The influence of radiation quality on the formation of DNA breaks.

We have aimed to present a comprehensive review of our understanding to date of the formation of DNA strand breaks induced by high LET radiation. We have discussed data obtained from DNA in solution as well as from the formation and "repair" of strand breaks in cell DNA. There is good agreement, qualitatively, between these two systems. Results were evaluated for two parameters: (1) effectivity per particle, the cross section (sigma) in micrometers 2/particle; and (2) the strand break induction frequency as number of breaks per Gy per unit DNA (bp or dalton). A series of biological effects curves (one for each Z-number) is obtained in effectivity versus LET plots. The relationships between induction frequencies of single-strand breaks, or double-strand breaks, or the residual "irrepairable" breaks and LET-values have been evaluated and discussed for a wide spectrum of heavy ions, both for DNA in solution and for DNA in the cell. For radiation induced total breaks in cell DNA, the RBE is less than one, while the RBE for the induction of DSBs can be greater than one in the 100-200 keV/micrometers range. The level of irrepairable strand breaks is highest in this same LET range and may reach 25 percent of the initial break yield. The data presented cover results obtained for helium to uranium particles, covering a particle incident energy range of about 2 to 900 MeV/u with a corresponding LET range of near 16 to 16000 keV/micrometers.     

Biological life support for manned missions by ESA.

The anticipated evolution of life support technologies for ESA, considering both the complementary life support system requirements and the missions' characteristics, is presented. Based on these results, promising biological life support technologies for manned space missions have been selected by ESA either for their intrinsic ability and performance in effecting specific tasks for atmosphere-, water-, waste-management versus physico-chemical alternatives and/or for longer-term application to a more ecological concept (CES) focusing ultimately on food production. Actual status and plan for terrestrial and space testing of biological life support presented focusing on the "task specific" decontamination technology of the Biological Air Filter (BAF), and on food reprocessing technologies from biodegradable wastes with the MELISSA microbial ecosystem.     

Wide field cameras for SAX

SAX is an Italian X-ray satellite with a Dutch contribution that will be placed in orbit in 1994. The prime scientific object of SAX is to cover an energy bandwidth that ranges from 0.1 keV up to 200 keV. Among other instruments, SAX will consist of two X-ray Wide Field Cameras built by the Space Research Organisation Netherlands at Utrecht. The WFCs are based on the coded mask principle, the reconstruction of the image takes place on ground. The field of view is 20 degrees square full width at half maximum (FWHM), the angular resolution 5 arcminutes (FWHM) and the energy band ranges from 1.8 to 30 keV with a resolution of 18% at 6 keV. The sensitive area is 200 cm² at 6 keV. The mask pattern is based on a pseudo random array with 255 × 257 elements of 1 mm², 50% of which are transparent.     

Plasma effects of active ion beam injections in the ionosphere at rocket altitudes

Data from ARCS rocket ion beam injection experiments will be primarily discussed in this paper. There are three results from this series of active experiments that are of particular interest in space plasma physics. These are the transverse acceleration of ambient ions in the large beam volume, the scattering of beam ions near the release payload, and the possible acceleration of electrons very close to the plasma generator which produce intense high frequency waves. The ability of 100 ma ion beam injections into the upper E and F regions of the ionosphere to produce these phenomena appear to be related solely to the process by which the plasma release payload and the ion beam are neutralized. Since the electrons in the plasma release do not convect with the plasma ions, the neutralization of both the payload and beam must be accomplished by large field-aligned currents (milliamperes/square meter) which are very unstable to wave growth of various modes. Future work will concentrate on the wave production and wave-particle interactions that produce the plasma/energetic particle effects discussed in this paper and which have direct application to natural phenomena in the upper ionosphere and magnetosphere.     

Observation of fundamental magnetoplasma emissions excited in magnetosphere by modulated electron beams

There were several cases when modulated electron beam had been injected from APEX satellite into an otherwise unmodified ionospheric plasma. The beams were formed from 2 μs pulses repeated at a rate of 40kHz. Injections took place in the altitude range 400–1100km over Europe. The onboard receiver connected to the dipole antenna swept the frequency interval 1–10 MHz in 1 sec. Due to a relatively narrow receiver bandwidth (15 kHz), moderate frequency step (50 kHz), slow changes of f_n and f_c (local electron plasma and gyro frequencies) fine structure of excited emissions was detected. In many cases, a very prominent doublet could be convincingly identified as a (f_n,f_u=sqrt(f_n^*f_n+f_c^*f_c)) band, with f_n practically equal to the local plasma frequency in the unperturbed ionosphere. Determination of plasma frequency is of prime importance in analysis of complex structures composed from various harmonics f_n,f_c,f_u. Complexity is manifested as coalescing of various harmonics with maxima shifted from nominal frequencies or splitting them into components due to mixing of local and propagation effects. Despite the existence of strong emissions on frequencies characteristic for the unperturbed ionosphere, very strong broadcasting transmissions are frequently cut off, even when the beam is directed upward. Most typical spectra are discussed.     

On the levels of enzymatic substrate specificity: implications for the early evolution of metabolic pathways.

The most frequently invoked explanation for the origin of metabolic pathways is the retrograde evolution hypothesis. In contrast, according to the so-called "patchwork" theory, metabolism evolved by the recruitment of relatively inefficient small enzymes of broad specificity that could react with a wide range of chemically related substrates. In this paper it is argued that both sequence comparisons and experimental results on enzyme substrate specificity support the patchwork assembly theory. The available evidence supports previous suggestions that gene duplication events followed by a gradual neoDarwinian accumulation of mutations and other minute genetic changes lead to the narrowing and modification of enzyme function in at least some primordial metabolic pathways.     

Psychosocial effects of adjustment in Antarctica: lessons for long-duration spaceflight

This paper examines the utility of remote, isolated Antarctic research stations as analogs for long-duration spaceflights from the perspective of psychosocial processes of adaptation and adjustment. Certain features of the physical and man-made environments found in Antarctica are similar to those that will be encountered in outer space. In both settings, men and women are likely to experience a number of physiological and psychological changes in response to the extreme environmental conditions and the prolonged isolation and confinement. Biomedical research in Antarctica provides an opportunity to study the causes of these changes and to develop strategies for reducing the risks to health and well-being before they pose a serious threat to crew safety and mission success. A number of lessons for long-duration spaceflight are examined, including screening and selection of personnel; training programs designed to facilitate individual adjustment and group adaptation and minimize group conflict; identification of optimal leadership characteristics for small, isolated groups; an understanding of social dynamics and group "microcultures" necessary for the organization and management of small but heterogeneous groups; organization of work activities; facility design; and support infrastructure.     

Lunar habitat concept employing the space shuttle external tank

The space shuttle external tank, which consists of a liquid oxygen tank, an intertank structure, and a liquid hydrogen tank, is an expendable structure used for approximately 8.5 min during each launch. A concept for outfitting the liquid oxygen tank-intertank unit for a 12-person lunar habitat is described. The concept utilizes existing structures and openings for both man and equipment access without compromising the structural integrity of the tank. Living quarters, instrumentation, environmental control and life support, thermal control, and propulsion systems are installed at Space Station Freedom. The unmanned habitat is then transported to low lunar orbit and autonomously soft landed on the lunar surface. Design studies indicate that this concept is feasible by the year 2000 with concurrent development of a space transfer vehicle and manned cargo lander for crew changeover and resupply.     

Radar Cross Section of Ships

A laboratory method to determine the magnitude and position of radar reflection sources on complex targets is described. In addition the method provides a way to measure the modification of the radar cross section (RCS) due to multipath. The method has application in modeling RCS for radar and electronic countermeasure (ECM) system performance analysis and in the study of the extent to which the signature of the target could be altered. The equipment described, termed MACROSCOPE, was developed for RCS studies by the U.S. Army and is described in limited distribution bution literature. The application to marine targets is new with this paper, as is the technique of measuring the RCS of parts of the target and analytically combining them to represent the whole. An illustration of the need for this type of laboratory equipment was illustrated by the extensive search for full scale data which could be compared to scale model data to validate the technique.