Extracting multiple frequencies from phase-only data

The problem of extracting multiple frequencies from phase-only data is addressed. Multiple frequency estimation is accomplished by reconstructing the Fourier transform of the complex-valued time signal and then finding peaks in the frequency domain. We present a set of conditions under which a discrete-time complex sequence can be completely specified by its phase-only information. Two candidate multiple frequency estimation schemes are introduced, one based on a closed-form least-squares inverse, the other an iterative reconstruction algorithm. The uniqueness of the closed-form solution and the convergence of the iterative scheme have been proven under certain conditions. Several examples are given, including the case where the phase is quantized as would happen in an analog-to-digital (A/D) converter. Extensions to the multidimensional case, and to the case of real-part only reconstruction are straightforward.     

Paramecium--a model system for studying cellular graviperception.

Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.     

The Freja ultraviolet imager

Auroral images acquired by satellite instrumentation have proven to be a crucial component of the scientific equiry into the physical processes of the Earth's magnetosphere. TheFreja mission provided an opportunity to extend these measurements into the temporal and spatial regime commonly associated with ground-based optical imagers. Employing the basic procedure used successfully in theViking program allowed image repetition rates of 6 s to be achieved with simultaneous exposure of all pixels within the field-of-view. Typical exposure times of 0.3 s required development of an improved image intensifier system and operational requirements resulted in image formats covering approximately one-third of a spin. Simultaneously exposing two broad-band UV cameras, the instrument generates in its normal operational mode 264.6 kbytes per image pair. Results from initial operations confirm the design approach and suggest such imagers can be routinely included on challenging low cost missions such asFreja.     

High Dynamic GPS Receiver Using Maximum Likelihood Estimationand Frequency Tracking

A new high dynamic global positioning system (GPS) receiver ispresented and its performance characterized by analysis,simulation, and demonstration. The demonstration receiver is abreadboard model capable of tracking a single simulated satellitesignal in pseudorange and range rate. Pseudorange and range rateestimates are made once every 20 ms, using a maximum likelihoodestimator, and are tracked by means of a third-order fadingmemory filter in a feedback configuration. The receiver trackspseudorange with rms errors of under 1 m when subjected tosimulated 50 g, 40 g/s circular trajectories. The tracking thresholdis approximately 28 dB·Hz, which provides 12 dB margin relativethe the minimum specified signal strength, assuming 3.5 dB systemnoise figure and 0 dB antenna gain.     

Century scale solar variability imprinted in the 44Ti activity in meteorites.

The measurements of gamma-activity of the 44Ti (T1/2 = 66.6 years) produced by spallation reaction of galactic cosmic rays (GCR) in Alfianello, Olivenza, Rio Negro, Dhajala and Torino meteorites, which fell in the time interval 1883 AD (Alfianello)-1988 AD (Torino), show a century scale modulation, connected to long-term solar-wind flux variations in the interplanetary space. The variation of the 44Ti activity with the time of fall of meteorites is qualitatively consistent with the Gleissberg solar cycle, but the amplitude is three-four times higher than expected (about 5%) for GCR flux modulated by solar activity, as determined solely by the sunspot number. The cosmogenic 44Ti is a suitable radioisotope for this investigation, but its activity in meteorites is very low. We performed these measurements in the underground low level counting station of Monte dei Cappuccini in Torino, by means of a big hyperpure Ge crystal (approximately 2 kg) in selective coincidence with a heavy NaI (T1) scintillation detector (approximately 28 kg). This system allows a reliable and non destructive measurement of 44Ti (44Sc) in meteorites of 200-1200 g in weight. The background is about 1 count per day in the gamma-peak at 1157 keV of 44Sc in equilibrium with its parent 44Ti. The high stability of the performance allows long-lasting runs (approximately 10(7) s) in order to attain results with a standard deviation up to about 10%.     

Large-size space laboratory for biological orbit experiments.

The study of space factors on living systems has great interest and long-term experiments during orbital flight will be important tool for increasing our knowledge. Realization of such experiments is limited by constraints of modern space stations. A new technology of large-size space laboratory for biological experiments has been developed on the basis of polymerization techniques. Using this technique there are no limits of form and size of laboratory for a space station that will permit long term experiments on Earth orbit with plants and animals in sufficient volume for creation of closed self-regulating ecological systems. The technology is based on experiments of the behavior of polymer materials in simulated free space conditions during the reaction of polymerization. The influences of space vacuum, sharp temperature changes and space plasma generated by galactic rays and Sun irradiation on chemical reaction were evaluated in their impact on liquid organic materials in laboratory conditions. The results of our study shows, that the chemical reaction is sensitive to such space factors. But we believe that the technology of polymerization could be used for the creation of space biological laboratories in Earth orbit in the near future.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.