DNA damage and repair in oncogenic transformation by heavy ion radiation.

Energetic heavy ions are present in galactic cosmic rays and solar particle events. One of the most important late effects in risk assessment is carcinogenesis. We have studied the carcinogenic effects of heavy ions at the cellular and molecular levels and have obtained quantitative data on dose-response curves and on the repair of oncogenic lesions for heavy particles with various charges and energies. Studies with repair inhibitors and restriction endonucleases indicated that for oncogenic transformation DNA is the primary target. Results from heavy ion experiments showed that the cross section increased with LET and reached a maximum value of about 0.02 micrometer2 at about 500 keV/micrometer. This limited size of cross section suggests that only a fraction of cellular genomic DNA is important in radiogenic transformation. Free radical scavengers, such as DMSO, do not give any effect on induction of oncogenic transformation by 600 MeV/u iron particles, suggesting most oncogenic damage induced by high-LET heavy ions is through direct action. Repair studies with stationary phase cells showed that the amount of reparable oncogenic lesions decreased with an increase of LET and that heavy ions with LET greater than 200 keV/micrometer produced only irreparable oncogenic damage. An enhancement effect for oncogenic transformation was observed in cells irradiated by low-dose-rate argon ions (400 MeV/u; 120 keV/micrometer). Chromosomal aberrations, such as translocation and deletion, but not sister chromatid exchange, are essential for heavy-ion-induced oncogenic transformation. The basic mechanism(s) of misrepair of DNA damage, which form oncogenic lesions, is unknown.     

涡旋真空泵的密封设计

涡旋真空泵在工作过程中动、静涡盘之间间隙存在泄漏损失,它是影响涡旋真空泵工作性能的重要因素;总结了涡旋真空泵的工作原理;介绍了涡旋真空泵的密封设计与选择、间隙的确定。从材料和结构两方面阐述了密封的几种结构形式,说明了各自的工作原理,及需要注意的问题,阐明这些问题是影响泵性能指标的重要因素。     

精仪工作台定量调整的原理及方法

论述了可调结构仪器工作台的结构、调整原理和调整方法，并以三点支撑结构、锥面支撑结构仪器工作台表面与测量轴线垂直度的调整为例，具体地阐述了调整方法和步骤。     

论民事诉讼举证时限制度之确立

《关于民事诉讼证据的若干规定》确立了我国的民事证据举证时限制度,克服了证据随时提出主义所造成的证据突袭、恶意拖延诉讼、浪费司法资源、破坏生效判决既判力、损害诉讼程序安定性等缺陷,但现行法律规定在适用时仍有一些不足,妨碍了举证时限制度作用的发挥,对此予以归纳并提出相对应的完善措施。     

企业标准体系的建立与完善及对策

介绍企业标准体系的基本构成、特征和建立的原则,就建立完善的企业技术标准体系、制定企业管理标准体系和工作标准等阐述体系建立的途径,并对企业标准体系的管理、完善和改进等有关的几个问题进行探讨。     

中小城市公交线路查询系统设计方法研究

论文在对首先阐述中小城市公交线路查询系统的需求分析、系统功能及性能要求描述的基础上具体讨论了系统设计、系统总体设计的思路和方法;最后给出了系统的测试分析和系统设计总结。     

薄膜充气管充气展开特性试验

用展开导轨消除因重力产生的摩擦力,在不同充气速率与初始展开角条件下对薄膜材料充气管的充气展开特性进行了试验研究,获得了展开角,以及展开加速度和内气压的变化规律。试验及分析结果表明:充气管的充气膨胀与展开相互独立,充气膨胀过程近似为等压增容过程,折叠部位的气压变化特性与整体气压变化特性差异明显。     

MAGNETOSPHERIC PHYSICS IN CHINA:2000-2002

This brief report presents the latest advances of the magnetospheric physics researches in China during the period of 2000-2002, made independently by Chinese space physicists and through international cooperation. The related areas cover almost every aspect of magnetospheric physics.     

减压活门性能试验台系统方案设计

主要介绍了减压活门性能试验台的实现机理、采用的方案。     

rom 15 sel

with primary goat embryonic fibroblast showed no difference from traditional feederlayer-based culture method used in mouse and human. The putative primary EG colonies were multilayer clumps ofcompact cel