Auxin polar transport in Arabidopsis under simulated microgravity conditions — Relevance to growth and development

Activity of auxin polar transport in inflorescence axes of Arabidopsis thaliana grown under simulated microgravity conditions was studied in relation to the growth and development. Seeds were germinated and allowed to grow on an agar medium in test tubes on a horizontal clinostat. Horizontal clinostat rotation substantially reduced the growth of inflorescence axes and the productivity of seeds of Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia), although it little affected seed germination, development of rosette leaves and flowering. The activity of auxin polar transport in inflorescence axes decreased when Arabidopsis plants were grown on a horizontal clinostat from germination stage, being ca. 60% of 1 g control. On the other hand, the auxin polar transport in inflorescence axes of Arabidopsis grown in 1 g conditions was not affected when the segments were exposed to various gravistimuli, including 3-dimensional clinorotation, during transport experiments. Pin-formed mutant of Arabidopsis, having a unique structure of the inflorescence axis with no flower and extremely low levels of the activity of auxin polar transport in inflorescence axes and endogenous auxin, did not continue its vegetative growth under clinostat rotation. These facts suggest that the development of the system of auxin polar transport in Arabidopsis is affected by microgravity, resulting in the inhibition of growth and development, especially during reproductive growth.     

Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

Communications and radio determination system using twogeostationary satellites. I. System and experiments

We developed two types of hybrid terminals that can provide both satellite communication and position determination services in one system. One terminal uses the single channel per carrier (SCPC) technique and the other uses the spread spectrum (SS) technique. To evaluate the performance of the two systems, we carried out experiments in Japan and in the Pacific Ocean using two geostationary satellites, ETS-V (150°E) and Inmarsat (180°E). The ranging accuracy between the mobile terminals and the base station via the satellites was found to be about 200 m using the SCPC system and about 10 m using the SS system. The measured positioning accuracy was about 1 km in the SCPC system and about 600 m in the SS system when experiments were carried out near Japan. The experimental results show that the positioning errors were mainly caused by the orbital determination errors of the two satellites. Presented here are the configurations and features of the SCPC and SS terminals, the experimental system, and the experimental results     

Thermoelastic Stability of Closed Cylindrical Shell in Supersonic Gas Flow

In a linear framework, the problem of stability of closed cylindrical shell is briefly discussed. The cylin- drical shell is immersed in a supersonic gas flow and under the influence of temperature field varying along the thickness. An unperturbed uniform velocity flow field, directed along the short edges of the shell, is applied. Due to the inhomogeneity of the temperature field distribution across the thickness shell buckling instability occurs. This instability accounts for the deformed shape of the shell, to be referred as the unperturbed state. Stability con- ditions and boundary for the unperturbed state of the system under consideration are presented following the basic theory of aero-thermo-elasticity. The stability boundary depends on the variables characterizing the flow speed, the temperature at the middIe plane of the shell and the temperature gradient in the direction normal to that plane. It is shown that the combined effect of the temperature field and flowing stream regulates the process of stability, and the temperature field can significantly change the flutter critical speed.     

Self-perspiration garment for extravehicular activity improves skin cooling effects without raising humidity

Introduction

The current U.S. extravehicular activity (EVA) suit in space includes liquid cooling and ventilation garment (LCVG) to control thermal condition. Tubes knitted in LCVG for flowing water interrupt evaporation of perspiration, and astronauts feel discomfort. In the present study, we hypothesized that a self-perspiration garment would effectively lower the skin temperature without raising humidity in the garment. Thus, we developed and examined the effects of the garment.

Methods

Eight healthy subjects were studied with a cyclic ergometer of 30, 60 90 and 120 W loading for 3 min each. Skin temperature and humidity on the back were measured continuously. Subjects wore and tested three types of garments i.e., a spandex wear without any cooling device (Normal), a simulated LCVG (s-LCVG) or the spandex wear knitted a vinyl tube for flowing water, and the spandex wear with a tube, which flows water and self-perspiration with oozing water for evaporative cooling (SPEC).

Results

All measurements were reached to steady state 2–3 min after the setting. The s-LCVG decreased skin temperature 0.39±0.14 °C during 12 min of cooling. With SPEC, skin temperature did not decrease significantly until 6–9 min after starting the cooling. However, the temperature decreased rapidly and significantly after that, and finally decreased 1.59±0.32 °C. Humidity in the SPEC was significantly lower than that in s-LCVG.

Discussion

SPEC was effective for lowering skin temperature without raising humidity in the garment. The concept is expected to use as a better cooling system during EVA.     

Determination of land surface temperature using precipitable water based Split-Window and Artificial Neural Network in Turkey

Land surface temperature (LST) calculation utilizing satellite thermal images is very difficult due to the great temporal variance of atmospheric water vapor in the atmosphere which strongly affects the thermal radiance incoming to satellite sensors. In this study, Split-Window (SW) and Radial Basis Function (RBF) methods were utilized for prediction of LST using precipitable water for Turkey. Coll 94 Split-Window algorithm was modified using regional precipitable water values estimated from upper-air Radiosond observations for the years 1990–2007 and Local Split-Window (LSW) algorithms were generated for the study area. Using local algorithms and Advanced Very High Resolution Radiometer (AVHRR) data, monthly mean daily sum LST values were calculated. In RBF method latitude, longitude, altitude, surface emissivity, sun shine duration and precipitable water values were used as input variables of the structure. Correlation coefficients between estimated and measured LST values were obtained as 99.23% (for RBF) and 94.48% (for LSW) at 00:00 UTC and 92.77% (for RBF) and 89.98% (for LSW) at 12:00 UTC. These meaningful statistical results suggest that RBF and LSW methods could be used for LST calculation.     

Calculation of fast neutron removal cross sections for different lunar soils

The interaction of galactic cosmic rays (GCRs) and solar energetic particles (SEPs) with the lunar surface produces secondary radiations as neutrons. The study of the production and attenuation of these neutrons in the lunar soil is very important to estimate the annual ambient dose equivalent on the lunar surface and for lunar nuclear spectroscopy. Also, understanding the attenuation of fast neutrons in lunar soils can help in measuring of the lunar neutron density profile and to measure the neutron flux on the lunar surface. In this paper, the attenuation of fast neutrons in different lunar soils is investigated. The macroscopic effective removal cross section (_ΣR)$({\mathrm{\Sigma }}_R)$ of fast neutrons was theoretically calculated from the mass removal cross-section values (_ΣR/ρ)$({\mathrm{\Sigma }}_R/\rho )$ for various elements in soils. The obtained values of (_ΣR)$({\mathrm{\Sigma }}_R)$ were discussed according to the density. The results show that the attenuation of fast neutrons is more important in the landing sites of Apollo 12 and Luna 16 than the other landing sites of Apollo and Luna missions.     

Stochastic processes for line shapes and intensities

Stochastic processes provide flexible and fast calculations for modeling dynamical interactions between an atom and charged particles. We use a stochastic renewal process for the plasma microfield being the cause of Stark broadening. The accuracy and improvement possibilities of Lyman profiles calculations with a renewal process are analyzed by comparing to ab initio simulations for ion broadening only. Stochastic processes may also be applied to out of equilibrium plasmas. We present our first results for the effect of Langmuir waves on a line broadened by electrons only, and for the changes of atomic populations submitted to strong temperature fluctuations.     

Nonlinear bending-torsion flutter experiments of a medium-aspect-ratio flexible wing model

In this research a two-motion medium-aspect-ratio straight flexible wing model was tested. Tests with the wing model were conducted in the subsonic wind tunnel of the Faculty of Aerospace Engineering at the Technion, Israel. Experimental results obtained for flutter and Limit Cycle Oscillations (LCO) of the wing model are presented. At least two types of flutter and postflutter LCO have been observed. Both types were characterized by coupling between the torsional and bending modes. The first type flutter was associated with coupling of the first bending and first torsional modes, whereas the second type was observed for coupling of the first torsional and second bending modes.