Gravitropic bending of fruiting bodies--a model based on hyphal gravisensing and cooperativity.

Gravitropic bending of the winter mushroom Flammulina velutipes is achieved by differential growth of the apical part of the stem, the transition zone. Ultrastructural analysis revealed that bending is due to the relaxation of tissue tensions at the lower flank of the stem where hyphal extension growth is promoted in contrast to the upper flank. Extension of lower flank hyphae is preceded by a conspicuous accumulation of microvesicles in the cytosol and their subsequent fusion with the vacuolar compartment, leading to a large volume increase. The hypothesis is put forward that all hyphae in the transition zone are capable of gravisensing. It is derived from experiments with transition zone segments, which exhibit negative gravitropic response independent from their origin within the stem. A model is presented which connects individual gravisensing of the hyphae with a cooperative response within the stem or small segments of the stem. An essential step is the transmission of positional information, by each hypha with respect to the gravitational vector, to the surroundings. The existence of a soluble growth regulator, which is enriched at the lower flank of the stem, is discussed. A gradient could be formed which precedes the gradient of microvesicle formation, and thereby determines the change of growth direction.     

The Neupert effect and new RHESSI measures of thetotal energy in electrons accelerated in solar flares

It is believed that a large fraction of the total energy released in a solar flare goes initially into acceleratedelectrons. These electrons generate the observed hard X-ray bremsstrahlung as they lose most of their energy by coulomb collisions in the lower corona and chromosphere. Results from the Solar Maximum Mission showed that there may be even more energy in accelerated electrons with energies above 25 keV than in the soft X-ray emitting thermal plasma. If this is the case, it is difficult to understand why the Neupert Effect — the empirical result that for many flares the time integral of the hard X-ray emission closely matches the temporal variation of the soft X-ray emission — is not more clearly observed in many flares. From recent studies, it appears that the fraction of the released energy going into accelerated electrons is lower, on average, for smaller flares than for larger flares. Also, from relative timing differences, about 25% of all flares are inconsistent with the Neupert Effect. The Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) is uniquely capable of investigating the Neupert Effec since it covers soft X-rays down to 3 keV (when both attenuators are out of the field of view) and hard X-rays with keV energy resolution, arcsecond-class angular resolution, and sub-second time resolution. When combined with the anticipated observations from the Soft X-ray Imager on the next GOES satellite, these observations will provide us with the ability to track the Neupert Effect in space and time and learn more about the relation between plasma heating and particle acceleration. The early results from RHESSI show that the electron spectrum extends down to as low as 10 keV in many flares, thus increasing the total energy estimates of the accelerated electrons by an order of magnitude or more compared with the SMM values. This combined with the possible effects of filling factors smaller than unity for the soft X-ray plasma suggest that there is significantly more energy in nonthermal electrons than in the soft X-ray emitting plasma in many flares.     

HUMEX, a study on the survivability and adaptation of humans to long-duration exploratory missions, part I: lunar missions.

The European Space Agency has recently initiated a study of the human responses, limits and needs with regard to the stress environments of interplanetary and planetary missions. Emphasis has been laid on human health and performance care as well as advanced life support developments including bioregenerative life support systems and environmental monitoring. The overall study goals were as follows: (i) to define reference scenarios for a European participation in human exploration and to estimate their influence on the life sciences and life support requirements; (ii) for selected mission scenarios, to critically assess the limiting factors for human health, wellbeing, and performance and to recommend relevant countermeasures; (iii) for selected mission scenarios, to critically assess the potential of advanced life support developments and to propose a European strategy including terrestrial applications; (iv) to critically assess the feasibility of existing facilities and technologies on ground and in space as testbeds in preparation for human exploratory missions and to develop a test plan for ground and space campaigns; (v) to develop a roadmap for a future European strategy towards human exploratory missions, including preparatory activities and terrestrial applications and benefits. This paper covers the part of the HUMEX study dealing with lunar missions. A lunar base at the south pole where long-time sunlight and potential water ice deposits could be assumed was selected as the Moon reference scenario. The impact on human health, performance and well being has been investigated from the view point of the effects of microgravity (during space travel), reduced gravity (on the Moon) and abrupt gravity changes (during launch and landing), of the effects of cosmic radiation including solar particle events, of psychological issues as well as general health care. Countermeasures as well as necessary research using ground-based test beds and/or the International Space Station have been defined. Likewise advanced life support systems with a high degree of autonomy and regenerative capacity and synergy effects were considered where bioregenerative life support systems and biodiagnostic systems become essential. Finally, a European strategy leading to a potential European participation in future human exploratory missions has been recommended.     

The effects of simulated microgravity on cultured chicken embryonic chondrocytes.

Using the cultured chicken embryonic chondrocytes as a model, the effects of simulated microgravity on the microtubular system of the cellular skeleton, extracellular matrix, alkaline phosphatase activity, intracellular free calcium concentration and mitochondrial ATP synthase activity with its oligomycin inhibition rate were studied with a clinostat. The microtubular content was measured by a flow cytometer. The decrease of microtubular content showed the impairment of the cellular skeleton system. Observation on the extracellular matrix by the scanning electron microscopy showed that it decreased significantly after rotating, and the fibers in the extracellular matrix were more tiny and disorderly than that of the control group. It can be concluded that the simulated microgravity can affect the secreting and assembly of the extracellular matrix. In contrast to the control, there was a time course decrease in alkaline phosphatase activity of chondrocytes, a marker of matrix mineralization. Meanwhile a significant drop in the intracellular calcium concentration happened at the beginning of rotation. These results indicate that simulated microgravity can suppress matrix calcification of cultured chondrocytes, and intracellular free calcium may be involved in the regulation of matrix calcification as the second signal transmitter. No significant changes happened in the mitochondrial ATP synthase activity and its oligomycin inhibition rate. Perhaps the energy metabolism wasn't affected by the simulated microgravity. The possible mechanisms about them were discussed.     

Overview of the space environmental effects observed on the retrieved Long Duration Exposure Facility (LDEF).

The Long Duration Exposure Facility (LDEF), which encompassed 57 experiments with more than 10,000 test specimens, spent 69 months in low Earth orbit (LEO) before it was retrieved by the Space Shuttle in January 1990. Hundreds of LDEF investigators, after studying for over two years these retrieved test specimens and the onboard recorded data and systems hardware, have generated a unique first-hand view of the long term synergistic effects that the LEO environment can have on spacecraft. These studies have also contributed significantly toward more accurate models of the LEO radiation, meteoroid, manmade debris and atomic oxygen environments. This paper provides an overview of some of the many LDEF observations and the implications these can have on future spacecraft such as Space Station Freedom.     

Demonstration of a zinc bromine battery in an electric vehicle

The zinc bromine battery is a high energy density battery that utilizes low cost materials. The battery is of unique construction utilizing plastic storage tanks for the zinc bromide electrolyte and plastic bipolar electrode stacks. This paper briefly describes the zinc bromine battery technology and the experience gained in installing and operating an electric vehicle with this advanced system. The described electric vehicle (The “T-Star”) was tested in March 1993 on the Chrysler Proving Grounds in Phoenix, Arizona and it participated in the May 1993 American Tour de Sol capturing second place over all and first place in the student division     

Out-of-ecliptic distribution of interplanetary dust derived from near earth flux

The out-of-ecliptic distribution of interplanetary dust, i.e. its number density, mainly has been subject to optical or infrared remote sensing techniques. As the population in interplanetary space is made up of orbiting particles which will cross the ecliptic plane, determination of their orbital properties there gives a possibility also to derive their out-of-ecliptic distribution. Determination of orbital elements is provided by advanced detectors capable of measuring the vector of impact velocity. In a simple model, which applies for advanced detectors in near earth orbit, the feasibility of the method to determine the out-of-ecliptic spatial distribution of dust has been tested.     

Calibration results obtained with Liulin-4 type dosimeters.

The Mobile Radiation Exposure Control System's (Liulin-4 type) main purpose is to monitor simultaneously the doses and fluxes at 4 independent places. It can also be used for personnel dosimetry. The system consists of 4 battery-operated 256-channel dosimeters-spectrometers. We describe results obtained during the calibrations of the spectrometers at the Cyclotron facilities of the University of Louvain, Belgium and of the National Institute of Radiological Sciences-STA, Chiba, Japan with protons of energies up to 70 MeV. The angular sensitivities of the devices are studied and compared with Monte-Carlo predictions. We also present the results obtained at the HIMAC accelerator with 500 MeV/u Fe ions and at the CERN high energy radiation reference fields. Records made during airplane flights are shown and compared with the predictions of the CARI-6 model.     

From Mercury to Pluto: A Common Approach to Mission Timekeeping

The successful launch of the New Horizons spacecraft for a rendezvous with Pluto and Charon and the continuing progress of the MESSENGER spacecraft toward Mercury now positions mankind to unlock mysteries of our solar system from Mercury to Pluto and beyond. Both missions, though very different in concept, use the same generic timekeeping system design. This paper explores how we maintain time on these spacecraft and how we establish on the ground the correlation between spacecraft time and Earth time. It further reviews the sub-millisecond correlation accuracy that has been demonstrated for the MESSENGER mission and the time accuracy we expect to achieve for that mission at Mercury and for the New Horizons mission at Pluto-Charon