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Inner wrinkling control in hydrodynamic deep drawing of an irregular surface part using drawbeads 总被引:2,自引:0,他引:2
Inner wrinkling phenomenon is more likely to develop during hydrodynamic deep drawing (HDD) of complicated component-forms due to the higher demand for controlling deformation sequences. Aiming at the problems in control of inner wrinkling for an irregular surface part featured with both concavity and convex, this research proposes an optimal design method of drawbead parameters to change the material flow. According to theoretical analysis of the mechanism of inner wrinkling, optimizing cavity pressure only is unreasonable to form a wrinkle-free deep-drawn part, so semi-circular drawbeads are employed. The effects of layout and height of drawbeads on forming results are discussed, and a process window is established based on evaluation indicators including the anti-wrinkle coefficient and the minimum wall thickness. Experiments are carried out to validate the process window, and the wall thickness and the wrinkle height are measured and compared with numerical findings. The results show that the anti-wrinkle ability of drawbeads weakens with increasing oblique angle and distance from the die center, while the wall thickness increases with increasing oblique angle and distance, and the inner wrinkling can be completely suppressed by drawbeads arranged in zones I and II with optimum penetration. 相似文献
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Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity 总被引:5,自引:0,他引:5
Recent studies have demonstrated that stem cells derived from adult hematopoietic tissues are capable of trans-differentiation into non-hematopoietic cells, and that the culture in microgravity (microg) may modulate the proliferation and differentiation. We investigated the application of microg to human umbilical cord blood stem cells (CBSC) in the induction of vascular endothelial phenotype expression and cellular proliferation. CD34+ mononuclear cells were isolated from waste human umbilical cord blood samples and cultured in simulated microg for 14 days. The cells were seeded in rotary wall vessels (RWV) with or without microcarrier beads (MCB) and vascular endothelial growth factor was added during culture. Controls consisted of culture in 1 G. The cell cultures in RWV were examined by inverted microscopy. Cell counts, endothelial cell and leukocyte markers performed by flow cytometry and FACS scan were assayed at days 1, 4, 7 and at the termination of the experiments. Culture in RWV revealed significantly increased cellular proliferation with three-dimensional (3D) tissue-like aggregates. At day 4, CD34+ cells cultured in RWV bioreactor without MCB developed vascular tubular assemblies and exhibited endothelial phenotypic markers. These data suggest that CD34+ human umbilical cord blood progenitors are capable of trans-differentiation into vascular endothelial cell phenotype and assemble into 3D tissue structures. Culture of CBSC in simulated microg may be potentially beneficial in the fields of stem cell biology and somatic cell therapy. 相似文献
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本文研究了一个光滑的、理想导电的、凸散射体的轮廓重构问题。主要从物理光学散射场的一阶修正项入手,分析了散射场的共极化和交叉极化信息与目标表面形状之间的关系,获得了散射场的极化特性与散射体表面上镜面点的主曲率密切相关的概念。借助于微分几何中曲面再现问题,重构出目标的外形图象。 相似文献
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