Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions

The oncogenic potential of high-energy ⁵⁶Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy ⁵⁶Fe is estimated to be 143 keV/μm with the exposure conditions used in this study. For ⁵⁶Fe ions, the maximum relative biological effectiveness (RBE_max) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/μm ⁴He nuclei, high-energy ⁵⁶Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E₁ analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy ⁵⁶Fe. Exposure of cells to misoprostol did not alter ⁵⁶Fe cytotoxicity or the rate of ⁵⁶Fe-induced oncogenic transformation.     

Controlling factors of Region 2 field-aligned current and its relationship to the ring current: Model results

One essential component of magnetosphere and ionosphere coupling is the closure of the ring current through Region 2 field-aligned current (FAC). Using the Comprehensive Ring Current Model (CRCM), which includes magnetosphere and ionosphere coupling by solving the kinetic equation of ring current particles and the closure of the electric currents between the two regions, we have investigated the effects of high latitude potential, ionospheric conductivity, plasma sheet density and different magnetic field models on the development of Region 2 field-aligned currents, and the relationship between R2 FACs and the ring current. It is shown that an increase in high latitude potential, ionospheric conductivity or plasma sheet density generally results in an increase in Region 2 FACs’ intensity, but R2 FACs display different local time and latitudinal distributions for changes in each parameter due to the different mechanisms involved. Our simulation results show that the magnetic field configuration of the inner magnetosphere is also an important factor in the development of Region 2 field-aligned current. More numerical experiments and observational results are needed in further our understanding of the complex relationship of the two current systems.     

Implication of multiple dipolarization event near 9 RE for the physics of substorms

In 2001, 2002 and 2003, the Polar spacecraft probed the near equatorial plasma sheet at 9 R_E near local midnight. Using the magnetic field observations, the signatures at substorm onsets are studied. Close to the flux pile-up region, the Polar spacecraft readily detects the dipolarization front, especially for pseudo onsets. An event with two distinct onsets has been examined. The signatures are found to be consistent with the multiple-onset model suggested by Russell [Russell, C.T. How northward turnings of the IMF can lead to substorm expansion onsets. Geophys. Res. Lett. 27, 3257–3259, 2000] which is a modified Near-Earth Neutral Line (NENL) model. Another similar event is also examined showing the effects of different Interplanetary Magnetic Field (IMF) conditions upon substorms. Moreover, ground effects can be very weak compared to in situ observations, especially for pseudo onsets, because these signatures appear to be localized and not global.     

Fault Detection in Dynamic Systems via Decision Fusion

Due to the growing demands for system reliability and availability of large amounts of data, efficient fault detection techniques for dynamic systems are desired. In this paper, we consider fault detection in dynamic systems monitored by multiple sensors. Normal and faulty behaviors can be modeled as two hypotheses. Due to communication constraints, it is assumed that sensors can only send binary data to the fusion center. Under the assumption of independent and identically distributed (1ID) observations, we propose a distributed fault detection algorithm, including local detector design and decision fusion rule design, based on state estimation via particle filtering. Illustrative examples are presented to demonstrate the effectiveness of our approach.     

STEREO Ground Segment, Science Operations, and Data Archive

Vitally important to the success of any mission is the ground support system used for commanding the spacecraft, receiving the telemetry, and processing the results. We describe the ground system used for the STEREO mission, consisting of the Mission Operations Center, the individual Payload Operations Centers for each instrument, and the STEREO Science Center, together with mission support from the Flight Dynamics Facility, Deep Space Mission System, and the Space Environment Center. The mission planning process is described, as is the data flow from spacecraft telemetry to processed science data to long-term archive. We describe the online resources that researchers will be able to use to access STEREO planning resources, science data, and analysis software. The STEREO Joint Observations Program system is described, with instructions on how observers can participate. Finally, we describe the near-real-time processing of the “space weather beacon” telemetry, which is a low telemetry rate quicklook product available close to 24 hours a day, with the intended use of space weather forecasting.     

Development of an Italian low cost GNSS/INS system universally suitable for mobile mapping

The first studies for the mobile mapping and creation of a vehicle for this kind of research was carried out by Canadian Researchers in the 1980s. Since then, these vehicles have been widely employed in several applications (road cadastre maps, terrestrial photogrammihetry, road sign recognition, etc.) for both commercial and research purposes throughout the world. Many GNSSIINS vehicles which can be equipped in different ways with one or more GPS, inertial sensors, and one or several cameras, have been realized. A characteristic shared by most of these devices concerns the high costs of the sensors, of the realization and of the maintenance. For this reason, a GNSSIINS system, that is suitable for any vehicle, made up of low cost devices (two GPS receivers, an INS, and a camera rigidly placed on a metallic bar), has been designed and built by our research group. Two tests run at different velocities have been carried out to evaluate the reliability of the system. After a presentation of the system, the differences that were witnessed during the application of these calibration methods are explained herein.     

The Juno Waves Investigation

Jupiter is the source of the strongest planetary radio emissions in the solar system. Variations in these emissions are symptomatic of the dynamics of Jupiter’s magnetosphere and some have been directly associated with Jupiter’s auroras. The strongest radio emissions are associated with Io’s interaction with Jupiter’s magnetic field. In addition, plasma waves are thought to play important roles in the acceleration of energetic particles in the magnetosphere, some of which impact Jupiter’s upper atmosphere generating the auroras. Since the exploration of Jupiter’s polar magnetosphere is a major objective of the Juno mission, it is appropriate that a radio and plasma wave investigation is included in Juno’s payload. This paper describes the Waves instrument and the science it is to pursue as part of the Juno mission.     

Range Contamination and Its Effect on Measurements

Increased sensitivity and dynamic range of the instrumental techniques used in conjunction with experiments on ballistic ranges have brought to the fore many problems arising from contamination in the ranges themselves. This is seldom discussed when experimental results are presented but is frequently the controlling limitation on the accuracy of the measurements. The authors discuss contamination due to dirt and debris resultant from gun operation, gaseous impurities, and projectile-borne impurities as they have occurred at the Re-entry Simulating Range of Lincoln Laboratory. The effects of these contaminants on measurements are discussed and illustrated, and measures for controlling them are outlined. Finally, a particular range operation is described from the standpoint of impurity control.     

The long term variability of 4U 1258-61 (GX304-1)

We present optical spectroscopy and photometry of 4U 1258-61 obtained over the course of six years. Evidence for the evolution of a circumstellar envelope throughout the course of the observations is presented.