Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Long term effects of low doses of 56Fe ions on the brain and retina of the mouse: ultrastructural and behavioral studies.

Eight month old male C57BL6 mice were exposed without anesthesia to whole-body irradiation in circular holders. The mice were tested for behavioral decrements after 0.5 and 50 rads of Fe particle irradiation at 6 and 12 months post irradiation to obtain long term results. A standard maze was used and the animals were timed for completion thereof. A string test also was administered to the mice, testing their ability to grasp and move along a string to safety. The results from animals exposed to 50 rads were significantly different from [correction of fron] control results to p = < .001 in both systems of testing. The hippocampus (believed to be the location of environmental interaction in the brain) and the retina were examined for ultrastructural changes. The ultrastructural changes were similar to those we found in our Cosmos 782, 936 and in our Argon experiments. The mouse data indicate that iron particles were able to induce long term changes in the central nervous system which lead to behavioral impairment.     

Transport modeling of energetic electrons in the inner magnetosphere with synchrotron energy losses

The resulting L-distributions and energy spectra of energetic magnetospheric electrons obtained from numerical solution of the radiation belt transport equation with and without accounting for electron synchrotron energy losses are compared. It is demonstrated that synchrotron losses play an important role in formation of the space and energetic distributions of electrons in the inner magnetosphere.     

Volatile Trapping in Martian Clathrates

Thermodynamic conditions suggest that clathrates might exist on Mars. Despite observations which show that the dominant condensed phases on the surface of Mars are solid carbon dioxide and water ice, clathrates have been repeatedly proposed to play an important role in the distribution and total inventory of the planet’s volatiles. Here we review the potential consequences of the presence of clathrates on Mars. We investigate how clathrates could be a potential source for the claimed existence of atmospheric methane. In this context, plausible clathrate formation processes, either in the close subsurface or at the base of the cryosphere, are reviewed. Mechanisms that would allow for methane release into the atmosphere from an existing clathrate layer are addressed as well. We also discuss the proposed relationship between clathrate formation/dissociation cycles and how potential seasonal variations influence the atmospheric abundances of argon, krypton and xenon. Moreover, we examine several Martian geomorphologic features that could have been generated by the dissociation of extended subsurface clathrate layers. Finally we investigate the future in situ measurements, as well as the theoretical and experimental improvements that will be needed to better understand the influence of clathrates on the evolution of Mars and its atmosphere.     

The Magnetic Electron Ion Spectrometer (MagEIS) Instruments Aboard the Radiation Belt Storm Probes (RBSP) Spacecraft

This paper describes the Magnetic Electron Ion Spectrometer (MagEIS) instruments aboard the RBSP spacecraft from an instrumentation and engineering point of view. There are four magnetic spectrometers aboard each of the two spacecraft, one low-energy unit (20–240 keV), two medium-energy units (80–1200 keV), and a high-energy unit (800–4800 keV). The high unit also contains a proton telescope (55 keV–20 MeV). The magnetic spectrometers focus electrons within a selected energy pass band upon a focal plane of several silicon detectors where pulse-height analysis is used to determine if the energy of the incident electron is appropriate for the electron momentum selected by the magnet. Thus each event is a two-parameter analysis, an approach leading to a greatly reduced background. The physics of these instruments are described in detail followed by the engineering implementation. The data outputs are described, and examples of the calibration results and early flight data presented.     

Climate Observations - The Instrumental Record

A survey is given of the available instrumental data for monitoring and analysis of climatic variations. We focus on temperature measurements, both over land and ocean, at the surface and aloft.Over land, the older observations were subject to exposure changes which may not have been fully compensated. The effects of urbanization have been largely avoided in studies of climatic change over the last 150 years. There are few records for pre-1850 outside Europe and eastern North America, and the global network shows a recent decline. Over the ocean, sea surface temperature (SST) has been measured using buckets, engine intakes, hull sensors, buoys, and satellites. Many of these data have been effectively homogenized, but new challenges arise as observing systems evolve. Available SST and marine air temperature datasets begin in the 1850s. The data are concentrated in shipping lanes especially before 1900, and very sparse during the world wars, but additional historical data are being digitized.The radiosonde record is short (40 years) and has major gaps over the oceans, tropics and Southern Hemisphere. Instrumental heterogeneities are beginning to be assessed and removed using physical and statistical techniques. The MSU record is complete but only began in 1979, and is not highly resolved in the vertical: major biases, mainly affecting the lower-tropospheric retrieval, have been reduced as a result of recent analyses.Advanced interpolation or data-assimilation techniques are being applied to these data, but the results must be interpreted with care.     

The IMPACT Solar Wind Electron Analyzer (SWEA)

SWEA, the solar wind electron analyzers that are part of the IMPACT in situ investigation for the STEREO mission, are described. They are identical on each of the two spacecraft. Both are designed to provide detailed measurements of interplanetary electron distribution functions in the energy range 1～3000 eV and in a 120°×360° solid angle sector. This energy range covers the core or thermal solar wind plasma electrons, and the suprathermal halo electrons including the field-aligned heat flux or strahl used to diagnose the interplanetary magnetic field topology. The potential of each analyzer will be varied in order to maintain their energy resolution for spacecraft potentials comparable to the solar wind thermal electron energies. Calibrations have been performed that show the performance of the devices are in good agreement with calculations and will allow precise diagnostics of all of the interplanetary electron populations at the two STEREO spacecraft locations.     

Neutral Atmospheres

This paper summarizes the understanding of aeronomy of neutral atmospheres in the solar system, discussing most planets as well as Saturn’s moon Titan and comets. The thermal structure and energy balance is compared, highlighting the principal reasons for discrepancies amongst the atmospheres, a combination of atmospheric composition, heliocentric distance and other external energy sources not common to all. The composition of atmospheres is discussed in terms of vertical structure, chemistry and evolution. The final section compares dynamics in the upper atmospheres of most planets and highlights the importance of vertical dynamical coupling as well as magnetospheric forcing in auroral regions, where present. It is shown that a first order understanding of neutral atmospheres has emerged over the past decades, thanks to the combined effects of spacecraft and Earth-based observations as well as advances in theoretical modeling capabilities. Key gaps in our understanding are highlighted which ultimately call for a more comprehensive programme of observation and laboratory measurements.     

Energy dependence of the characteristic decay time of proton fluxes in solar cosmic ray events

Energetic solar proton events within the energy interval 1–48 MeV at the stage of their decay are considered over the period of 1974–2001. The dependence of the characteristic decay time on the proton energy in the assumed power-law representation τ(E) =E ^?n is analyzed for the events with an exponential decay form. The dependence of n on the heliolongitude of the flare (the particles source on the Sun) is studied.