DNA fragmentation by charged particle tracks.

High-LET (linear energy transfer) charged particles induce DNA double-strand breaks (DSB) in a non-random fashion in mammalian cells. The clustering of DSB, probably determined by track structure as well as chromatin conformation, results in an excess of small- and intermediate-sized DNA fragments. DNA fragmentation in normal human fibroblasts (GM5758) was analyzed by pulsed-field gel electrophoresis after irradiation with photons (60Co) or 125 keV/micrometers nitrogen ions. Compared to conventional DSB analysis, i.e. assays only measuring the fraction of DNA smaller than a single threshold, the relative biological effectiveness (RBE) for DSB induction increased with 100%. Further, the size distribution of DNA fragments showed a significant dependence on radiation quality, with an excess of fragments up to 1 Mbp. Irradiation of naked genomic DNA without histone proteins increased the DSB yields 25 and 13 times for photons and nitrogen ions, respectively. The results suggest possible roles of both track structure and chromatin organization in the distribution of DNA double-strand breaks along the chromosome.     

High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth.

The effect of high temperatures (35 and 45 degrees C) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degrees C for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degrees C for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation.     

Gravisensing: ionic responses, cytoskeleton and amyloplast behavior.

In Zea mays L., changes in orientation of stems are perceived by the pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. Gravity is perceived in the bundle sheath cells, which contain amyloplasts that sediment to the new cell base when a change in the gravity vector occurs. The mechanism by which the mechanical signal is transduced into a physiological response is so far unknown for any gravity perceiving tissue. It is hypothesized that this involves interactions of amyloplasts with the plasma membrane and/or ER via cytoskeletal elements. To gain further insights into this process we monitored amyloplast movements in response to gravistimulation. In a pharmacological approach we investigated how the dynamics of plastid sedimentation are affected by actin and microtubule (MT) disrupting drugs. Dark grown caulonemal filaments of the moss Physcomitrella patens respond to gravity vector changes with a reorientation of tip growth away from the gravity vector. MT distributions in tip cells were monitored over time and MTs were seen to accumulate preferentially on the lower flank of the tip 30 min after a 90 degree turn. Using a self-referencing Ca2+ selective ion probe, we found that growing caulonemal filaments exhibit a Ca2+ influx at the apical dome, similar to that reported previously for other tip growing cells. However, in gravistimulated Physcomitrella filaments the region of Ca2+ influx is not confined to the apex, but extends about 60 micrometers along the upper side of the filament. Our results indicate an asymmetry in the Ca2+ flux pattern between the upper and side of the filament suggesting differential activation of Ca2+ permeable channels at the plasma membrane.     

Analysis of the Cyclotron Facility calibration and aircraft dosimetry results from the LIULIN-3M instrument.

The LIULIN-3M instrument is a further development of the LIULIN dosimeter-radiometer, used on the MIR spacestation during the 1988-1994 time period. The LIULIN-3M is designed for continuous monitoring of the radiation environment during the BION-12 satellite flight in 1999. A semiconductor detector with 1 mm thickness and cm2 area is contained in the instrument. Pulse high analysis technique is used to determine the energy losses in the detector. The final data from the instrument are the flux and the dose rate for the exposure time and 256 channels of absorbed dose spectra based on the assumption that the particle flux is normal to the detector. The LIULIN-3M instrument was calibrated by proton fluxes with different energies at the Indiana University Cyclotron Facility in June 1997 and had been used for radiation measurements during commercial aircraft flights. The calibration procedure and some flight results are presented in this paper.     

Indirect search for Dark Matter with H.E.S.S.

Observations of the Galactic center region with the H.E.S.S. telescopes have established the existence of a steady, extended source of gamma-ray emission coinciding with the position of the super massive black hole Sgr A^*. This is a remarkable finding given the expected presence of dense self-annihilating Dark Matter in the Galactic center region. The self-annihilation process is giving rise to gamma-ray production through hadronization including the production of neutral pions which decay into gamma-rays but also through (loop-suppressed) annihilation into final states of almost mono-energetic photons. We study the observed gamma-ray signal (spectrum and shape) from the Galactic center in the context of Dark Matter annihilation and indicate the prospects for further indirect Dark Matter searches with H.E.S.S.     

A space radiation transport method development.

Improved spacecraft shield design requires early entry of radiation constraints into the design process to maximize performance and minimize costs. As a result, we have been investigating high-speed computational procedures to allow shield analysis from the preliminary design concepts to the final design. In particular, we will discuss the progress towards a full three-dimensional and computationally efficient deterministic code for which the current HZETRN evaluates the lowest-order asymptotic term. HZETRN is the first deterministic solution to the Boltzmann equation allowing field mapping within the International Space Station (ISS) in tens of minutes using standard finite element method (FEM) geometry common to engineering design practice enabling development of integrated multidisciplinary design optimization methods. A single ray trace in ISS FEM geometry requires 14 ms and severely limits application of Monte Carlo methods to such engineering models. A potential means of improving the Monte Carlo efficiency in coupling to spacecraft geometry is given in terms of re-configurable computing and could be utilized in the final design as verification of the deterministic method optimized design.     

The role of radial perpendicular diffusion and latitude dependent acceleration along the solar wind termination shock

A numerical model, based on Parker’s transport equation, describing the modulation of anomalous cosmic rays and containing diffusive shock acceleration is applied. The role of radial perpendicular diffusion at the solar wind termination shock, and as the dominant diffusion coefficient in the outer heliosphere, is studied, in particular the role it plays in the effectiveness of the acceleration of anomalous protons and helium when its latitude dependence is changed. It is found that the latitudinal enhancement of radial perpendicular diffusion towards the heliospheric poles and along the termination shock has a prominent effect on the acceleration of these particles. It results in a ‘break’ in the energy spectrum for anomalous protons at ∼6.0 MeV, causing the spectral index to change from E^−1.38 to E^−2.23, but for anomalous helium at ∼3.0 MeV, changing the spectral index from E^−1.38 to E^−2.30. When approaching the simulated TS, the changes in the modulated spectra as they unfold to a ‘steady’ power law shape at energies below 50 MeV are much less prominent as a function of radial distances when radial perpendicular diffusion is increased with heliolatitude.     

Robust model following control of parallel buck converters

A robust model-following (RMF) control technique for average current mode controlled (ACC) parallel buck dc-dc converters, RMFACC, is presented. RMFACC achieves that the loop gain of the voltage loop is little sensitive to the variation of power stage parameters: number of modules, input voltage, load, and component tolerances. The design of the voltage loop is 'decoupled' from the design of the disturbance rejection transfer functions in an important degree, so that the output impedance and audio susceptibility are greatly reduced without the need of high loop gain crossover frequencies. A comparative study between conventional ACC and RMFACC is shown.