Soybean cultivar selection for Bioregenerative Life Support Systems (BLSSs) – Hydroponic cultivation

Four soybean cultivars (‘Atlantic’, ‘Cresir’, ‘Pr91m10’ and ‘Regir’), selected through a theoretical procedure as suitable for cultivation in BLSS, were evaluated in terms of growth and production. Germination percentage and Mean Germination Time (MGT) were measured. Plants were cultivated in a growth chamber equipped with a recirculating hydroponic system (Nutrient Film Technique). Cultivation was performed under controlled environmental conditions (12 h photoperiod, light intensity 350 μmol m⁻² s⁻¹, temperature regime 26/20 °C light/dark, relative humidity 65–75%). Fertigation was performed with a standard Hoagland solution, modified for soybean specific requirements, and EC and pH were kept at 2.0 dS m⁻¹ and 5.5 respectively.     

Current collection through the transport of electrons across magnetic field lines

Collection of electrons by a long conducting cylinder in a flowing plasma is studied by means of numerical simulations. The plasma flow simulates the relative motion between a spacecraft and plasma. The sheath structures and the levels of electron current collections for the cases with and without an ambient magnetic field ( ) are studied. It is found that for the flow perpendicular to the magnetic field, the current is considerably enhanced depending on the relative drift velocity. In the case of a non-zero magnetic field perpendicular to the cylinder axis, the potential structure is a two-dimensional double layer with dimensions L L_|, where L and L_| are the dimensions perpendicular and parallel to , respectively. L is found to be the current limiting radius given by the Parker-Murphy model. For the flow along , the electron current is found to be smaller than that for the flow perpendicular to . This is explained in terms of the potential structures.     

Mapping lunar surface chemistry: New prospects with the Chandrayaan-2 Large Area Soft X-ray Spectrometer (CLASS)

Surface chemistry of airless bodies in the solar system can be derived from remote X-ray spectral measurements from an orbiting spacecraft. X-rays from planetary surfaces are excited primarily by solar X-rays. Several experiments in the past have used this technique of X-ray fluorescence for deriving abundances of the major rock forming elements. The Chandrayaan-2 orbiter carries an X-ray fluorescence experiment named CLASS that is designed based on results from its predecessor C1XS flown on Chandrayaan-1. We discuss the new aspects of lunar science that can be potentially achieved with CLASS.     

Occurrence of equatorial plasma bubbles over Kolhapur

This paper reports the nightglow observations of OI 630.0 nm emissions, made by using all sky imager operating at low latitude station Kolhapur (16.8°N, 74.2°E and dip lat. 10.6°N) during high sunspot number years of 24th solar cycle. The images are analyzed to study the nocturnal, seasonal and solar activity dependence occurrence of plasma bubbles. We observed EPBs in images regularly during a limited period 19:30 to 02:30 LT and reach maximum probability of occurrence at 22:30 LT. The observation pattern of EPBs shows nearly no occurrence during the month of May and it maximizes during the period October–April. The equinox and solstice seasonal variations in the occurrence of plasma bubbles show nearly equal and large differences, respectively, between years of 2010–11 and 2011–12.     

High resolution time profile of decimetric type-III bursts

Type-III bursts are signatures of the electron beams accelerated during the solar flares, their observation and investigation provide information of the acceleration processes, the characteristics of the exciting agent and the acceleration site. The Brazilian Solar Spectroscope (BSS), in operation at INPE, Brazil, have recorded type-III radio bursts in decimetric range (2050–2250 MHz) with high time resolution of 20 ms. Decimetric reverse drift bursts are possibly generated in a dense loop by electron beams travelling towards the photosphere. Hence their time profiles should carry signatures of the density inhomogenities in the loop. Here the temporal and spectral characteristics of decimetric type-III bursts are presented.     

Possible applications of aquatic bioregenerative life support modules for food production in a Martian base.

Water is the essential precondition of life in general and also for the establishment of a Martian base suitable for long duration stays of humans. It is not yet proven if there is indeed a "frozen ocean" under the surface of Mars but if this could be verified it would open innovative aspects for the construction of bioregenerative life support systems (BLSS). In a general concept higher plants will play the predominant role in a Martian BLSS. It is not clear, however, how these will grow and bring seed in reduced gravity and there may be differences in the productivity in comparison to Earth conditions. Therefore, organisms which are already adapted to low gravity conditions, namely non-gravitropic aquatic plants and also aquatic animals may be used to enhance the functionality of the Martian BLSS as a whole. It has been shown already with the so-called C.E.B.A.S. MINIMODULE in the STS-89 and STS-90 spaceshuttle missions that the water plant Ceratophyllum demersum has an undisturbed and high biomass production under space conditions. Moreover, the teleost fish species Xiphophorus helleri adapted easily to the micro-g environment and maintained its normal reproductive functions. Based on this findings a possible scenario is presented in which aquatic plant production modules and combined animal-plant production systems may be used for human food production and water and air regeneration in a Martian base.     

Induced abnormality in Mir- and Earth grown Super Dwarf wheat.

Super-dwarf wheat grown on the Mir space station using the Svet "Greenhouse" exhibited morphological, metabolic and reproductive abnormalities compared with Earth-grown wheat. Of prominent importance were the abnormalities associated with reproductive ontogeny and the total absence of seed formation on Mir. Changes in the apical meristem associated with transition from the vegetative phase to floral initiation and development of the reproductive spike were all typical of 'Super-Dwarf' wheat up to the point of anthesis. Observation of ruptured anthers from the Mir-grown plants revealed what appeared to be normally developed pollen. These pollen gains, however, contained only one nuclei, while normal viable pollen is tri-nucleate. A potentially important difference in the flight experiment, compared with ground reference studies, was the presence of a high level of atmospheric ethylene (1,200 ppb). Ground studies conducted by exposing 'Super-Dwarf' wheat to ethylene just prior to anthesis resulted in manifestation of the same abnormalities observed in the space flight samples.     

High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth.

The effect of high temperatures (35 and 45 degrees C) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degrees C for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degrees C for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation.     

Aquatic modules for bioregenerative life support systems: developmental aspects based on the space flight results of the C.E.B.A.S. MIN-MODULE.

The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) is an artificial aquatic ecosystem which contains teleost fishes, water snails, ammonia oxidizing bacteria and edible non-gravitropic water plants. It serves as a model for aquatic food production modules which are not seriously affected by microgravity and other space conditions. Its space flight version, the so-called C.E.B.A.S. MINI-MODULE was already successfully tested in the STS-89 and STS-90 (NEUROLAB) missions. It will be flown a third time in space with the STS-107 mission in January 2003. All results obtained so far in space indicate that the basic concept of the system is more than suitable to drive forward its development. The C.E.B.A.S. MINI-MODULE is located within a middeck locker with limited space for additional components. These technical limitations allow only some modifications which lead to a maximum experiment time span of 120 days which is not long enough for scientifically essential multi-generation-experiments. The first necessary step is the development of "harvesting devices" for the different organisms. In the limited space of the plant bioreactor a high biomass production leads to self-shadowing effects which results in an uncontrolled degradation and increased oxygen consumption by microorganisms which will endanger the fishes and snails. It was shown already that the latter reproduce excellently in space and that the reproductive functions of the fish species are not affected. Although the parent-offspring-cannibalism of the ovoviviparous fish species (Xiphophorus helleri) serves as a regulating factor in population dynamics an uncontrolled snail reproduction will also induce an increased oxygen consumption per se and a high ammonia concentration in the water. If harvesting locks can be handled by astronauts in, e. g., 4-week intervals their construction is not very difficult and basic technical solutions are already developed. The second problem is the feeding of the animals. Although C.E.B.A.S.-based aquaculture modules are designed to be closed food loop systems (edible herbivorous fish species and edible water plants) which are already verified on Earth this will not be possible in space without devices in which the animals are fed from a food storage. This has to be done at least once daily which would waste too much crew time when done by astronauts. So, the development of a reliable automated food dispenser has highest priority. Also in this case basic technical solutions are already elaborated. The paper gives a comprehensive overview of the proposed further C.E.B.A.S.-based development of longer-term duration aquatic food production modules.