Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Neuritogenesis: a model for space radiation effects on the central nervous system.

Pivotal to the astronauts' functional integrity and survival during long space flights are the strategies to deal with space radiations. The majority of the cellular studies in this area emphasize simple endpoints such as growth related events which, although useful to understand the nature of primary cell injury, have poor predictive value for extrapolation to more complex tissues such as the central nervous system (CNS). In order to assess the radiation damage on neural cell populations, we developed an in vitro model in which neuronal differentiation, neurite extension, and synaptogenesis occur under controlled conditions. The model exploits chick embryo neural explants to study the effects of radiations on neuritogenesis. In addition, neurobiological problems associated with long-term space flights are discussed.     

The observation of the galactic anticenter region by the balloon borne gamma-ray telescope Natalya-I

This paper presents the results of measurements of gamma radiation with energies above 5 MeV, from the galactic anticenter region. The balloon-borne gamma ray telescope “Natalya-I”, was launched on 6 November, 1980 from the Tata Institute of Fundamental Research's Balloon Facility (Hyderabad, India) and reached ceiling altitude of 35 km. The results on the accelerator calibration of the telescope, using a “tagged” gamma ray beam are also presented.     

INMARSAT integrity channels for global navigation satellite systems

The transmission of integrity information using a signal format compatible with the Global Positioning System (GPS) and relayed through a geostationary satellite repeater, which will be critical in achieving high integrity and availability of global navigation by satellite is discussed. The inclusion of navigation repeaters designed to fulfil this function, the next generation of INMARSAT spacecraft, INMARSAT-3 is examined. The global navigation satellite system (GNSS) integrity channel (GIC) will employ pseudorandom codes in the same family as, but distinct from, the codes reserved by GPS. The data format of the basic integrity channel is designed to convey user range error information for 24 to 40 satellites. A closed-loop timing compensation technique will be used at the uplinking Earth station, to make the signal's clock and carrier Doppler variations identical to those that would result from an onboard signal source. Therefore, the INMARSAT-3 satellites will increase the number of useful navigation satellites available to any user, and can also function as sources of precise timing. There is also a possibility that wide area differential corrections can be carried on the same signal     

Initial results from COMPTEL onboard GRO

COMPTEL is the first imaging telescope to explore the MeV gamma-ray range (0.7 to 30 MeV). At present, it is performing a complete sky survey. In later phases of the mission selected celestial objects will be studied in more detail. The data from the first year of the mission have demonstrated that COMPTEL performs very well. First sky maps of the inner part of the Galaxy clearly identify the plane as a bright MeV-source (probably due to discrete sources as well as diffuse radiation). The Crab and Vela pulsar lightcurves have been measured with unprecedented accuracy. The quasars 3C273 and 3C279 have been seen for the first time at MeV energies. Both quasars show a break in their energy spectra in the COMPTEL energy range. The 1.8 MeV line from radioactive ²⁶A1 has been detected from the central region of the Galaxy and a first sky map of the inner part of the Galaxy has been obtained in the light of this line. Upper limits to gamma-ray line emission at 847 keV and 1.238 MeV from SN 1991T have been derived. Upper limits to the interstellar gamma-ray emissivity have been determined at MeV-energies. Several cosmic gamma-ray bursts within the field-of-view have been located with an accuracy of about 1°. On 1991 June 9, 11 and 15, COMPTEL observed gamma-ray emission (continuum and line) from three solar flares. Also neutrons were detected from the June 9 and June 15 flares.     

Pulsar studies with GRO-COMPTEL

Pulsar measurements performed by the experiment COMPTEL, aboard the Compton Gamma Ray Observatory, are described. The main results refer to the Crab and Vela pulsars whose pulse shape characteristics are given in some detail and light curves are compared with those above 50 MeV, as observed by the COS-B satellite. No other gamma-ray pulsars have been detected to date by COMPTEL, the upper limit on the pulsed signal from Geminga being compatible with indications by other experiments.     

The structural features of the germanium-silicon solid solution crystals obtained under microgravity

Structural features of germanium-silicon solid solution crystals have been investigated and silicon distribution in the crystals has been studied. All the crystals obtained under microgravity had, in spite of good external shape and facetting, a poorer internal structure than those obtained on Earth. The distribution of silicon have been shown to be non-uniform. High dislocation densities were observed.     

Beam-plasma discharge in near rocket region

The model of beam-plasma discharge near the rocket following electron beam injection into the ionosphere is presented. The dependence of discharge plasma density on neutral atmosphere concentration and beam parameters is found. Radioemission and luminiscence intensities from discharge are estimated. The model is consistent with the data of ARAKS and “Zarnitsa-2” experiments.     

Wave measurements in active experiments on plasma beam injection

The paper presents the results and discussion of VLF wave measurements carried out in the course of two rocket experiments on injection of a dense cesium ion beam into the ionosphere at the heights of 165–240 km. The injection was accompanied by enhancement of the broad-band noise by several orders of magnitude. The wave measurements in the frequency range of 1–11 kHz revealed two pronounced frequencies somewhat exceeding that of the lower-hybrid resonance oscillations in the background plasma and the cesium beam. The oscillations were modulated by frequencies close to the ion-cyclotron frequencies of the background plasma.     

Measurements of LET spectra and comparison to models.

We present measurements of LET spectra for near earth orbits with various inclinations and altitudes. A comparison with calculated LET spectra shows that the contribution from direct ionizing galactic cosmic rays is well described by the models. An additional contribution to the spectra originates from stopping protons and from nuclear interactions of particles with material. In the case of an interaction a large amount of energy is deposited in a small volume by target recoils or target fragments. These events will be called short range (SR) events. For a low inclination orbit radiation belt protons are the main source of these events while galactic protons become more important when increasing the inclination to near polar orbits. We show that the contribution of SR events for orbits with low altitude (324 km) and 57 degrees inclination is comparable to that for an orbit with 28 degrees inclination at a high altitude (510 km).