Population dynamics of transgenic microorganisms in the different microecosystem conditions.

The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap (r) Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km (r) Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. Grant numbers: 99-04-96017, 25, 00-07-9011.     

A Series of Proton Flares in July 1985 from Observations by the Prognoz-10 Satellite and Vega-1, 2 Spacecraft

The enhancements of solar protons with an energy of more than 30 MeV, originating from flares in one active region and observed simultaneously aboard three spacecraft Vega-1, 2 and Prognoz-10 in July 1985, are analyzed and approximated in this work by using the reflection model [2]. The numerical values of several physical parameters (the parameters of distribution of solar cosmic ray (SCR) particles inside heliosphere traps, the coefficients of trap transparency) that are not observed by direct measurements are estimated.     

ATE enabling technologies

A discussion of the current and emerging core technologies and philosophies that will enable Air Force personnel to quickly, accurately and intuitively diagnose faults in increasingly complex systems     

A physical and chemical characterization of Martian permafrost as a possible habitat for viable microorganisms.

Data from experiments with model samples show that ion transfer coefficients in the water-rich permafrost on Mars must be three orders of magnitude less than those of terrestrial permafrost. The effects of low temperatures and of carbon dioxide have been accounted for. Exchange between cells and the environment is impeded in Martian permafrost. The microscopic distributional heterogeneity of concentration, pH, Eh, and other physicochemical parameters may be more pronounced in the permafrost of Mars. We present a classification of unfrozen water types in the permafrost that is based on the structures of unfrozen water films and on their functions with respect to cells. Any viable microorganisms on Mars probably exist with minimum metabolism in compact zones with energy carriers and high transfer coefficients. These zones may be microvolumes of unfrozen water in which cells accumulate.     

Venus Express: Scientific goals, instrumentation, and scenario of the mission

The first European mission to Venus (Venus Express) is described. It is based on a repeated use of the Mars Express design with minor modifications dictated in the main by more severe thermal environment at Venus. The main scientific task of the mission is global exploration of the Venusian atmosphere, circumplanetary plasma, and the planet surface from an orbiting spacecraft. The Venus Express payload includes seven instruments, five of which are inherited from the missions Mars Express and Rosetta. Two instruments were specially designed for Venus Express. The advantages of Venus Express in comparison with previous missions are in using advanced instrumentation and methods of remote sounding, as well as a spacecraft with a broad spectrum of capabilities of orbital observations.     

The Motion of Coaxial Bodies of Varying Composition on the Active Leg of Descent

The motion of a spacecraft (SC) with double rotation and variable mass on the active leg of its descent is considered. The SC consists of two coaxial bodies. The coaxial scheme is used for gyroscopic stabilization of the SC longitudinal axis by the method of partial spin-up. The equations of spatial motion of coaxial bodies of varying composition are derived and approximate solutions for the angles of spatial orientation are found. The condition of decreasing amplitude of nutation oscillations is obtained, which allows the estimation of efficiency of the stabilization by partial spin-up. The errors in the magnitude and direction of the vector of braking thrust are also determined.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 3, 2005, pp. 224–232.Original Russian Text Copyright © 2005 by Aslanov, Doroshin, Kruglov.     

Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Neuritogenesis: a model for space radiation effects on the central nervous system.

Pivotal to the astronauts' functional integrity and survival during long space flights are the strategies to deal with space radiations. The majority of the cellular studies in this area emphasize simple endpoints such as growth related events which, although useful to understand the nature of primary cell injury, have poor predictive value for extrapolation to more complex tissues such as the central nervous system (CNS). In order to assess the radiation damage on neural cell populations, we developed an in vitro model in which neuronal differentiation, neurite extension, and synaptogenesis occur under controlled conditions. The model exploits chick embryo neural explants to study the effects of radiations on neuritogenesis. In addition, neurobiological problems associated with long-term space flights are discussed.     

Relaxation of electron and proton radiation belts of the earth after strong magnetic storms

During strong magnetic storms in July and November of 2004 the fluxes of trapped particles (protons and electrons of MeV energies) in the Earth’s radiation belts have increased by orders of magnitude and then decreased remaining on an enhanced level for several months. These enhancements allowed us to study the processes of relaxation of the radiation belts. Measurements of energetic particles by low-altitude satellites Coronas-F and Servis-1 have shown that predictions of the theory about the rate of pitch-angle diffusion are not always correct, giving both overestimated and underestimated values for the lifetime of energetic particles.