Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms.

The space radiation environment is a mixed field consisting of different particles having different energies, including high charge and energy (HZE) ions. Conventional measurements of absorbed doses may not be sufficient to completely characterise the radiation field and perform reliable estimates of health risks. Biological dosimetry, based on the observation of specific radiation-induced endpoints (typically chromosome aberrations), can be a helpful approach in case of monitored exposure to space radiation or other mixed fields, as well as in case of accidental exposure. Furthermore, various ratios of aberrations (e.g. dicentric chromosomes to centric rings and complex exchanges to simple exchanges) have been suggested as possible fingerprints of radiation quality, although all of them have been subjected to some criticisms. In this context a mechanistic model and a Monte Carlo code for the simulation of chromosome aberration induction were developed. The model, able to provide dose-responses for different aberrations (e.g. dicentrics, rings, fragments, translocations, insertions and other complex exchanges), was further developed to assess the dependence of various ratios of aberrations on radiation quality. The predictions of the model were compared with available data, whose experimental conditions were faithfully reproduced. Particular attention was devoted to the scoring criteria adopted in different laboratories and to possible biases introduced by interphase death and mitotic delay. This latter aspect was investigated by taking into account both metaphase data and data obtained with Premature Chromosome Condensation (PCC).     

Aquatic modules for bioregenerative life support systems: developmental aspects based on the space flight results of the C.E.B.A.S. MIN-MODULE.

The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) is an artificial aquatic ecosystem which contains teleost fishes, water snails, ammonia oxidizing bacteria and edible non-gravitropic water plants. It serves as a model for aquatic food production modules which are not seriously affected by microgravity and other space conditions. Its space flight version, the so-called C.E.B.A.S. MINI-MODULE was already successfully tested in the STS-89 and STS-90 (NEUROLAB) missions. It will be flown a third time in space with the STS-107 mission in January 2003. All results obtained so far in space indicate that the basic concept of the system is more than suitable to drive forward its development. The C.E.B.A.S. MINI-MODULE is located within a middeck locker with limited space for additional components. These technical limitations allow only some modifications which lead to a maximum experiment time span of 120 days which is not long enough for scientifically essential multi-generation-experiments. The first necessary step is the development of "harvesting devices" for the different organisms. In the limited space of the plant bioreactor a high biomass production leads to self-shadowing effects which results in an uncontrolled degradation and increased oxygen consumption by microorganisms which will endanger the fishes and snails. It was shown already that the latter reproduce excellently in space and that the reproductive functions of the fish species are not affected. Although the parent-offspring-cannibalism of the ovoviviparous fish species (Xiphophorus helleri) serves as a regulating factor in population dynamics an uncontrolled snail reproduction will also induce an increased oxygen consumption per se and a high ammonia concentration in the water. If harvesting locks can be handled by astronauts in, e. g., 4-week intervals their construction is not very difficult and basic technical solutions are already developed. The second problem is the feeding of the animals. Although C.E.B.A.S.-based aquaculture modules are designed to be closed food loop systems (edible herbivorous fish species and edible water plants) which are already verified on Earth this will not be possible in space without devices in which the animals are fed from a food storage. This has to be done at least once daily which would waste too much crew time when done by astronauts. So, the development of a reliable automated food dispenser has highest priority. Also in this case basic technical solutions are already elaborated. The paper gives a comprehensive overview of the proposed further C.E.B.A.S.-based development of longer-term duration aquatic food production modules.     

2000年和2001年52°N地区OI5577气辉强度与原子氧浓度峰值的时间变化特征

分析了2000年和2001年期间52°N地区OI5577气辉强度的夜间变化特征和季节变化特征．利用由 OI5577气辉强度反演原子氧浓度峰值的方法反演出原子氧浓度的峰值,分析了峰值的夜间变化特征和季节变化特征．结果表明, OI5577气辉强度的夜间变化特征随季节变化, 2000年春季的夜间强度最大值出现在0000LT 之后,夏季和秋季的出现在0000LT之前,冬季的出现在0000LT,2001年春季和秋季的夜间强度最大值出现在0000LT之前,夏季和冬季的出现在0000LT;OI5577气辉强度在2000年2月份,8月份和10月份出现最大值,在2001年9月份有最大值．就主要特征而言,反演出的原子氧浓度峰值的夜间变化特征和季节变化特征分别与OI5577气辉强度的一致．     

The Scanning Sky Monitor (SSM) on ASTROSAT

The Scanning Sky Monitor is one of the experiments onboard the ASTROSAT, an Indian multiwavelength astronomy satellite mission. This experiment will detect and monitor X-ray transients in the energy band 2–10 keV. It is similar in design to the ASM on RXTE. It consists of position-sensitive proportional counters with one-dimensional mask. We describe the configuration of the experiment. We also discuss some of the results obtained using a detector which has already been fabricated and tested in our laboratory.     

Keck observations of ROSAT ultra-luminous X-ray source candidates

We present results of optical follow-up observations of candidate ultra-luminous X-ray sources (ULXs). Using Keck optical spectroscopy, 17 of the candidates from the Colbert and Ptak [Colbert, E.J.M., Ptak, A.F. A catalog of candidate intermediate-luminosity X-ray objects. ApJS 143, 25–45, 2002] catalog have been identified; this is one of the largest sets of optical identifications of such objects thus far. Fifteen are background active galactic nuclei (AGN); two are foreground stars in our Galaxy. These findings are consistent with background and foreground object expectations, as derived from log N–log S relations. Also, the results are briefly discussed in terms of the spiral-galaxy/ULX connection.     

Two years of INTEGRAL observation of the BHC IGR J17464-3213

On March 2003, IBIS, the γ-ray imager on board the INTEGRAL satellite, detected an outburst from a new source, IGR J17464-3213, that turned out to be an HEAO-1 transient, namely H1743-322. The spectral and temporal evolutions of the source were observed by INTEGRAL in different periods. Also RXTE observed the source for the first time on 2003 March 29 during a PCA Galactic bulge scan. The source flux decayed below the RXTE PCA sensitivity limit in November 2003, then in April 2004 it was again detected by INTEGRAL. On July 3, 2004 the source was again detected by RXTE/PCA at a 2–10 keV intensity of 16 mCrab and on July 7, reached 69 mCrab. Recently, a new outburst was observed on August 2005. We briefly summarise here the behaviour of the source observed by INTEGRAL from March 2003 to August 2005. The new outbursts of the source and the analysis of all the data collected (now public) give a global view of the spectral and time behaviour of this X-ray transient.     

Multiband echo tomography of Sco X-1

We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λ_c  6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc.     

Changes in surface UV solar irradiance and ozone over the balkans during the eclipse of August 11, 1999

Intensive measurements of UV solar irradiance, total ozone and surface ozone were carried out during the solar eclipse of 11 August 1999 at Thessaloniki, Greece and Stara Zagora, Bulgaria, located very close to the footprint of the moon's shadow during the solar eclipse with the maximum coverage of the solar disk reaching about 90% and 96% respectively. It is shown that during the eclipse the diffuse component is reduced less compared to the decline of the direct solar irradiance at the shorter wavelengths. A 20-minute oscillation of erythemal UV-B solar irradiance was observed before and after the time of the eclipse maximum under clear skies, indicating a possible 20-minute fluctuation in total ozone presumably caused by the eclipse induced gravity waves. The surface ozone measurements at Thessaloniki display a decrease of around 10–15 ppbv during the solar eclipse. Similarly, ozone profile measurements with a lidar system indicate a decrease of ozone up to 2 km during the solar eclipse. The eclipse offered the opportunity to test our understanding of tropospheric ozone chemistry. The use of a chemical box model suggested that photochemistry can account for a significant portion of the observed surface ozone decrease.     

FEM code simulation of the magnetospheric proton fluxes

We report a study of the numeric solution to the diffusive transport equation for energetic protons magnetically trapped in the Earth's equatorial magnetosphere. The analysis takes into account the pertinent physical processes in this region, including deceleration of protons by Coulomb collisional interactions with free and bound electrons, the charge exchange process, cosmic ray albedo neutron decay source, and electric and magnetic radial diffusion. These results were obtained using the Finite Element Method with magnetic moment and geomagnetic L-shell as free variables. Steady state boundary conditions were imposed at L=1 as zero distribution function and at L=7 with proton distribution function extracted from ATS 6 satellite observations. The FEM-code yields unidirectional proton flux in the energy range of 0.1–1000 MeV at the equatorial top of the geomagnetic lines, and the results are found to be in satisfactorily agreement with the empirical NASA AP-8 model proton flux within the energy range of 0.5–100 MeV. Below 500 keV, the empirical AP-8 model proton fluxes are several orders of magnitude greater than those computed with the FEM-code at L<3. This discrepancy is difficult to explain by uncertainties of boundary spectrum parameters or transport coefficients.     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.