A biophysical model for estimating the frequency of radiation-induced mutations resulting from chromosomal translocations.

Gene mutations can be induced by radiation as a result of chromosomal translocations. A biophysical model is developed to estimate the frequency of this type of mutation induced by low-LET radiation. Mutations resulting from translocations are assumed to be formed by misrejoining of two DNA double strand breaks (DSB), one within the gene and one on a different chromosome. The chromosome containing the gene is assumed to occupy a spherical territory and does not overlap spatially with other chromosomes. Misrejoining between two DSB can occur only if the two DSB are closer than an interaction distance at the time of their induction. Applying the model to mutations of the hprt gene induced in G0 human lymphocyte cells by low-LET radiation, it is calculated that mutations resulting from translocations account for about 14% of the total mutations. The value of the interaction distance is determined to be 0.6 micrometers by comparing with the observed frequency of translocations in the X-chromosome.     

Fallacies in computational testing of matrix positivedefiniteness/semidefiniteness

The status of computational tests for establishing matrix positive semidefiniteness and positive definiteness is reviewed. Two pervasive real-time tests that have been used for many years in varied applications to ensure that computed covariances encountered in Kalman filter applications are positive definite and discussed. Structural representations of covariance matrices are reviewed as a prelude to constructing a counterexample and demonstrating that it refutes these real-time tests. It is maintained that the latter are bogus approaches despite the fact that they are pervasive. It is suggested that such bogus tests arose as an attempt to fill the need for a quick check (over the entire mission time) of the massive number of matrices computationally encountered in real-time applications     

Accelerating hypersonic airplanes with ground-power

The possible benefits of using ground power to accelerate the airplane on a launch ramp are quantified. A passenger-carrying hypersonic airplane leaving its loading ramp carries fuel for acceleration, cruise and landing. For example, a 230-passenger plane starting a Mach 5 trans-Pacific flight will be loaded with 80 metric tons of liquid hydrogen, up to 40% of which is burned in generating kinetic energy. Catapulting this airplane to cruise speed with ground power would reduce weight and liquid-hydrogen volume. However, there are limits. A ramp for launching a 150-metric-ton vehicle to Mach 5 with 4 g acceleration would be 38 km long and require up to 10 GW of power. In the final second, 2.8 MWh of energy is consumed along 1720 m of ramp. Possible scenarios and acceleration mechanisms are evaluated     

Magnetic resonance image enhancement using V-filter

A method for enhancing the boundaries of magnetic resonance images (MRIs) using a V-filter (a spatial nonlinear filter) is presented. By iterated V-filtering the signal-to-noise ratio of the MRI can be increased while preserving edge sharpness. A simulation experiment was carried out to evaluate the filter, using clinical brain images. The boundaries of the brain tumor and edematous region were precisely extracted by the region segmentation techniques     

Issues in deep space radiation protection.

The exposures in deep space are largely from the Galactic Cosmic Rays (GCR) for which there is as yet little biological experience. Mounting evidence indicates that conventional linear energy transfer (LET) defined protection quantities (quality factors) may not be appropriate for GCR ions. The available biological data indicates that aluminum alloy structures may generate inherently unhealthy internal spacecraft environments in the thickness range for space applications. Methods for optimization of spacecraft shielding and the associated role of materials selection are discussed. One material which may prove to be an important radiation protection material is hydrogenated carbon nanofibers.     

Effects of altered gravity on the swimming behaviour of fish.

Humans taking part in parabolic aircraft flights (PAFs) may suffer from space motion sickness-phenomena (SMS, a kinetosis). It has been argued that SMS during PAFs might not be based on microgravity alone but rather on changing accelerations from 0 g to 2 g. We test here the hypothesis that PAF-induced kinetosis is based on asymmetric statoliths (i.e., differently weighed statoliths on the right and the left side of the head), with asymmetric inputs to the brain being disclosed at microgravity. Since fish frequently reveal kinetotic behaviour during PAFs (especially so-called spinning movements and looping responses), we investigated (1) whether or not kinetotically swimming fish at microgravity would have a pronounced inner ear otolith asymmetry and (2) whether or not slow translational and continuously changing linear (vertical) acceleration on ground induced kinetosis. These latter accelerations were applied using a specially developed parabel-animal-container (PAC) to stimulate the cupular organs. The results suggest that the fish tested on ground can counter changing accelerations successfully without revealing kinetotic swimming patterns. Kinetosis could only be induced by PAFs. This finding suggests that it is indeed microgravity rather than changing accelerations, which induces kinetosis. Moreover, we demonstrate that fish swimming kinetotically during PAFs correlates with a higher otolith asymmetry in comparison to normally behaving animals in PAFs.     

Effects of hypergravity on the development of cell number and asymmetry in fish brain nuclei.

Larval cichlid fish (Oreochromis mossambicus) siblings were subjected to 3 g hypergravity (hg) and total darkness for 21 days during development and subsequently processed for conventional histology. Further siblings reared at 1 g and alternating light/dark (12h:12h) conditions served as controls. Cell number counts of the visual Nucleus isthmi (Ni) versus the vestibular Nucleus magnocellularis (Nm) revealed that in experimental animals total cell number was decreased in the Ni, possibly due to retarded growth as a result of the lack of visual input whereas no effect was observed in the Nm. Calculating the percentual asymmetry in cell number (i.e., right vs. the left side of the brain), no effects of hg/darkness were seen in the Ni, whereas asymmetry was slightly increased in the Nm. Since the asymmetry of inner ear otoliths is decreased under hg, this finding may indicate efferent vestibular action of the CNS on the level of the Nm by means of a feedback mechanism.     

Calibration results obtained with Liulin-4 type dosimeters.

The Mobile Radiation Exposure Control System's (Liulin-4 type) main purpose is to monitor simultaneously the doses and fluxes at 4 independent places. It can also be used for personnel dosimetry. The system consists of 4 battery-operated 256-channel dosimeters-spectrometers. We describe results obtained during the calibrations of the spectrometers at the Cyclotron facilities of the University of Louvain, Belgium and of the National Institute of Radiological Sciences-STA, Chiba, Japan with protons of energies up to 70 MeV. The angular sensitivities of the devices are studied and compared with Monte-Carlo predictions. We also present the results obtained at the HIMAC accelerator with 500 MeV/u Fe ions and at the CERN high energy radiation reference fields. Records made during airplane flights are shown and compared with the predictions of the CARI-6 model.     

Analytic example of a Schweppe likelihood-ratio detector

A low-dimensional test problem with a known solution is used to verify various computer implementations of F.C. Schweppe's likelihood detector (1965). In this case a closed-form solution is provided for a Schweppe likelihood detector in terms of an intermediate Kalman filter, as utilized in its implementation, for detecting the presence of a two-state signal model in Gaussian white noise. The associated error probabilities are also evaluated following a procedure that utilizes optimized Chernoff-like bounds for a tight approximation. A methodology is demonstrated for appropriately setting the decision threshold for this example as a tradeoff against allowable observation time. By using this or similar examples, certain qualitative and quantitative aspects of the software implementation can be checked for conformance to anticipated behavior as an intermediate benchmark, prior to modular replacement of the various high-order matrices appropriate to the particular application