Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

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Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.     

Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

Interplanetary shocks and sudden impulses during solar maximum (2000) and solar minimum (1995–1996)

In this work a study is performed on the correlation between fast forward interplanetary shock parameters at 1 Astronomical Unit and sudden impulse (SI) amplitudes in the H-component of the geomagnetic field, for periods of solar activity maximum (year 2000) and minimum (year 1995–1996). Solar wind temperature, density and speed, and total magnetic field, were taken to calculate the static pressures (thermal and magnetic) both in the upstream and downstream sides of the shocks. The variations of the solar wind parameters and pressures were then correlated with SI amplitudes. The solar wind speed variations presented good correlations with sudden impulses, with correlation coefficients larger than 0.70 both in solar maximum and solar minimum, whereas the solar wind density presented very low correlation. The parameter better correlated with SI was the square root dynamic pressure variation, showing a larger correlation during solar maximum (r = 0.82) than during solar minimum (r = 0.77). The correlations of SI with square root thermal and magnetic pressure were smaller than with the dynamic pressure, but they also present a good correlation, with r > 0.70 during both solar maximum and minimum. Multiple linear correlation analysis of SI in terms of the three pressure terms have shown that 78% and 85% of the variance in SI during solar maximum and minimum, respectively, are explained by the three pressure variations. Average sudden impulse amplitude was 25 nT during solar maximum and 21 nT during solar minimum, while average square root dynamic pressure variation is 1.20 and 0.86 nPa^1/2 during solar maximum and minimum, respectively. Thus on average, fast forward interplanetary shocks are 33% stronger during solar maximum than during solar minimum, and the magnetospheric SI response has amplitude 20% higher during solar maximum than during solar minimum. A comparison with theoretical predictions (Tsyganenko’s model corrected by Earth’s induced currents) of the coefficient of sudden impulse change with solar wind dynamic pressure variation showed excellent agreement, with values around 17 nT/nPa^1/2.     

Artificial gravity: a possible countermeasure for post-flight orthostatic intolerance

Four payload crewmembers were exposed to sustained linear acceleration in a centrifuge during the Neurolab (STS-90) flight. In contrast to previous studies, otolith–ocular reflexes were preserved during and after flight. This raised the possibility that artificial gravity may have acted as a countermeasure to the deconditioning of otolith–ocular reflexes. None of the astronauts who were centrifuged had orthostatic intolerance when tested with head-up passive tilt after flight. Thus, centrifugation may also have helped maintain post-flight hemodynamic responses to orthostasis by preserving the gain of the otolith–sympathetic reflex. A comparison with two fellow Neurolab orbiter crewmembers not exposed to artificial gravity provided some support for this hypothesis. One of the two had hemodynamic changes in response to post-flight tilt similar to orthostatically intolerant subjects from previous missions. More data is necessary to evaluate this hypothesis, but if it were proven correct, in-flight short-radius centrifugation may help counteract orthostatic intolerance after space flight.