Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Cluster observations showing the indication of the formation of a modified-two-stream instability in the geomagnetic tail

This study presents several observations of the Cluster spacecraft on September 24, 2003 around 15:10 UT, which show necessary prerequisites and consequences for the formation of the so-called modified-two-stream instability (MTSI). Theoretical studies suggest that the plasma is MTSI unstable if (1) a relative drift of electrons and ions is present, which exceeds the Alfvèn speed, and (2) this relative drift or current is in the cross-field direction. As consequences of the formation of a MTSI one expects to observe (1) a field-aligned electron beam, (2) heating of the plasma, and (3) an enhancement in the B-wave spectrum at frequencies in the range of the lower-hybrid-frequency (LHF). In this study we use prime parameter data of the CIS and PEACE instruments onboard the Cluster spacecraft to verify the drift velocities of ions and electrons, FGM data to calculate the expected LHF and Alfvèn velocity, and the direction of the current. The B-wave spectrum is recorded by the STAFF instrument of Cluster. Finally, a field aligned beam of electrons is observed by 3D measurements of the IES instrument of the RAPID unit. Observations are verified using a theoretical model showing the build-up of a MTSI under the given circumstances.     

Doppler observations of infrasonic waves of meteorological origin at ionospheric heights

Ionospheric effects of meteorological origin observed by the continuous HF Doppler sounder over the Czech Republic are reported in this paper. We focused on detection of waves of periods 1–10 min. We discuss the influence of dynamics and intensity of active weather systems on the occurrence of short period waves and dependence of the observed ionospheric effects on the height of reflection of the sounding radio wave. We observed 3–5 min waves during a severe weather event in summer and 2.5–4 min waves during a severe weather event in winter. We excluded possible geomagnetic origin of these oscillations by the analysis of fluctuations of the local geomagnetic field. In eight cases of 10, wave activity in the analysed period range was not significantly increased comparing to quiet days. The intensity of weather systems as well as the location of potential sources of waves towards the points of HF Doppler shift observation influence significantly the occurrence of infrasonic waves in the ionosphere. The results in Central Europe differ considerably from those previously obtained in North America. As a possible reason, we discuss different intensity and dynamics of weather systems in both regions.     

Survival of dormant organisms after long-term exposure to the space environment

The RF SRC—Institute of Biomedical Problems, Russian Academy of Sciences, developed Biorisk hardware to study the effects of long-term exposure of dormant forms of various organisms to outer space and used it to complete a series of experiments on the Russian Module (RM) of the International Space Station (ISS).The experiments were performed using prokaryotes (Bacillus bacteria) and eukaryotes (Penicillium, Aspergillus, and Cladosporium fungi), as well as spores, dormant forms of higher plants, insects, lower crustaceans, and vertebrates. The biological samples were housed in two containers that were exposed to outer space for 13 or 18 months. The results of the 18-month experiment showed that, in spite of harsher temperature than in the first study, most specimens remained viable.These experiments provided evidence that not only bacterial and fungal spores but also dormant forms of organisms that reached higher levels of evolutionary development had the capability to survive a long-term exposure to outer space. This observation suggests that they can be transferred on outer walls of space platforms during interplanetary missions.     

Radial velocity search for low mass companions of nearby stars

The detection of substellar companions of nearby stars is very difficult, because these objects are very faint and are located close to their bright parent stars. One way to attack this problem is to look for small periodic motion of the bright star, induced by the gravitational attraction exerted by the unseen small companion. Over the past decade stellar spectroscopy has been used by a few teams to monitor the radial velocities of several samples of stars, with a threshold for detection of companions well below the substellar limit of about 0.08 M. So far, only a few possible spectroscopic binaries have been identified where the unseen companions might be substellar.Recently, radio observers discovered that the millisecond pulsar PSR B1257+12 displays periodic variations in the pulse arrival times, indicating the existence of at least three unseen companions in circular orbits around the pulsar. This is the first convincing case for an extra-solar planetary system. This discovery suggests that planetary systems can form in very different situations, and therefore may prove to be common.     

Bacterial growth at the high concentrations of magnesium sulfate found in martian soils

The martian surface environment exhibits extremes of salinity, temperature, desiccation, and radiation that would make it difficult for terrestrial microbes to survive. Recent evidence suggests that martian soils contain high concentrations of MgSO? minerals. Through warming of the soils, meltwater derived from subterranean ice-rich regolith may exist for an extended period of time and thus allow the propagation of terrestrial microbes and create significant bioburden at the near surface of Mars. The current report demonstrates that halotolerant bacteria from the Great Salt Plains (GSP) of Oklahoma are capable of growing at high concentrations of MgSO? in the form of 2 M solutions of epsomite. The epsotolerance of isolates in the GSP bacterial collection was determined, with 35% growing at 2 M MgSO?. There was a complex physiological response to mixtures of MgSO? and NaCl coupled with other environmental stressors. Growth also was measured at 1 M concentrations of other magnesium and sulfate salts. The complex responses may be partially explained by the pattern of chaotropicity observed for high-salt solutions as measured by agar gelation temperature. Select isolates could grow at the high salt concentrations and low temperatures found on Mars. Survival during repetitive freeze-thaw or drying-rewetting cycles was used as other measures of potential success on the martian surface. Our results indicate that terrestrial microbes might survive under the high-salt, low-temperature, anaerobic conditions on Mars and present significant potential for forward contamination. Stringent planetary protection requirements are needed for future life-detection missions to Mars.     

The Brazilian scientific microsatellite SACI-1

The National Space Research Institute (INPE) is developing the first Brazilian Scientific Microsatellite (SACI-1) based on the vanguard technology and on the experience acquired through projects developed by Brazilian Space Program. The SACI-1 is a 750km polar orbit satellite. The spacecraft will combine spin stabilization with geomagnetic control and has a total mass of 60 kg. The overall dimensions are 640×470×470 mm. The SACI-1 satellite shall be launched together with CBERS (China-Brazil Earth Resource Satellite). Its platform is being designed for multiple mission applications. The Brazilian Academy of Sciences has selected four scientific payloads that characterize the mission. The scientific experiments are: ORCAS (Solar and Anomalous Cosmic Rays Observation in the Magnetosphere), PLASMEX (Study of Plasma Bubbles), FOTSAT (Airglow Photometer), and MAGNEX (Geomagnetic Experiment).     

Feasibility of performing space surveillance tasks with a proposed space-based optical architecture

Under ESA contract an industrial consortium including Aboa Space Research Oy (ASRO), the Astronomical Institute of the University of Bern (AIUB), and the Dutch National Aerospace Laboratory (NLR), proposed the observation concept, developed a suitable sensor architecture, and assessed the performance of a space-based optical (SBO) telescope in 2005. The goal of the SBO study was to analyse how the existing knowledge gap in the space debris population in the millimetre and centimetre regime may be closed by means of a passive optical instrument. The SBO instrument was requested to provide statistical information on the space debris population in terms of number of objects and size distribution. The SBO instrument was considered to be a cost-efficient with 20 cm aperture and 6° field-of-view and having flexible integration requirements. It should be possible to integrate the SBO instrument easily as a secondary payload on satellites launched into low-Earth orbits (LEO), or into geostationary orbit (GEO). Thus the selected mission concept only allowed for fix-mounted telescopes, and the pointing direction could be requested freely. Since 2007 ESA focuses space surveillance and tracking activities in the Space Situational Awareness (SSA) preparatory program. Ground-based radars and optical telescopes are studied for the build-up and maintenance of a catalogue of objects. In this paper we analyse how the proposed SBO architecture could contribute to the space surveillance tasks survey and tracking. We assume that the SBO instrumentation is placed into a circular sun-synchronous orbit at 800 km altitude. We discuss the observation conditions of objects at higher altitude, and select an orbit close to the terminator plane. A pointing of the sensor orthogonal to the orbital plane with optimal elevation slightly in positive direction (0° and +5°) is found optimal for accessing the entire GEO regime within one day, implying a very good coverage of controlled objects in GEO, too. Simulations using ESA’s Program for Radar and Optical Observation Forecasting (PROOF) in the version 2005 and a GEO reference population extracted from DISCOS revealed that the proposed pointing scenario provides low phase angles together with low angular velocities of the objects crossing the field-of-view. Radiometric simulations show that the optimal exposure time is 1–2 s, and that spherical objects in GEO with a diameter of below 1 m can be detected. The GEO population can be covered under proper illumination nearly completely, but seasonal drops of the coverage are possible. Subsequent observations of objects are on average at least every 1.5 days, not exceeding 3 days at maximum. A single observation arc spans 3° to 5° on average. Using a simulation environment that connects PROOF to AIUB’s program system CelMech we verify the consistency of the initial orbit determination for five selected test objects on subsequent days as a function of realistic astrometric noise levels. The initial orbit determination is possible. We define requirements for a correlator process essential for catalogue build-up and maintenance. Each single observation should provide an astrometric accuracy of at least 1”–1.5” so that the initially determined orbits are consistent within a few hundred kilometres for the semi-major axis, 0.01 for the eccentricity, and 0.1° for the inclination.     

The microbial habitability of weathered volcanic glass inferred from continuous sensing techniques

Basaltic glasses (hyaloclastite) are a widespread habitat for life in volcanic environments, yet their interior physical conditions are poorly characterized. We investigated the characteristics of exposed weathered basaltic glass from a surface outcrop in Iceland, using microprobes capable of continuous sensing, to determine whether the physical conditions in the rock interior are hospitable to microbial life. The material provided thermal protection from freeze-thaw and rapid temperature fluctuations, similar to data reported for other rock types. Water activity experiments showed that at moisture contents less than 13% wet weight, the glass and its weathering product, palagonite, had a water activity below levels suitable for bacterial growth. In pore spaces, however, these higher moisture conditions might be maintained for many days after a precipitation event. Gas exchange between the rock interior and exterior was rapid (< 10 min) when the rocks were dry, but when saturated with water, equilibration took many hours. During this period, we demonstrated the potential for low oxygen conditions within the rock caused by respiratory stimulation of the heterotrophic community within. These conditions might exist within subglacial environments during the formation of the rocks or in micro-environments in the interior of exposed rocks. The experiments showed that microbial communities at the site studied here could potentially be active for 39% of the year, if the depth of the community within the outcrop maintains a balance between access to liquid water and adequate protection from freezing. In the absence of precipitation, the interior of weathered basaltic glass is an extreme and life-limiting environment for microorganisms on Earth and other planets.     

The Effect of Interfering Signals on the Performance of Angle of Arrival Estimates

The performance of angle of arrival estimates using an array in the presence of interfering signals is evaluated using the Cramer-Rao bound. To do this, a model for interference is developed which presents the interference as narrowband, temporally white, but spatially correlated, noise. The bound is evaluated and it is shown to depend upon the ambiguity function of the array and spatial correlation matrix of the noise plus interference. Motivation of the model in the context of air traffic control and sonar surveillance is presented.