Automorphogenesis and gravitropism of plant seedlings grown under microgravity conditions.

Plant seedlings exhibit automorphogenesis on clinostats. The occurrence of automorphogenesis was confirmed under microgravity in Space Shuttle STS-95 flight. Rice coleoptiles showed an inclination toward the caryopsis in the basal region and a spontaneous curvature in the same adaxial direction in the elongating region both on a three-dimensional (3-D) clinostat and in space. Both rice roots and Arabidopsis hypocotyls also showed a similar morphology in space and on the 3-D clinostat. In rice coleoptiles, the mechanisms inducing such an automorphic curvature were studied. The faster-expanding convex side of rice coleoptiles showed a higher extensibility of the cell wall than the opposite side. Also, in the convex side, the cell wall thickness was smaller, the turnover of the matrix polysaccharides was more active, and the microtubules oriented more transversely than the concave side, and these differences appear to be causes of the curvature. When rice coleoptiles grown on the 3-D clinostat were placed horizontally, the gravitropic curvature was delayed as compared with control coleoptiles. In clinostatted coleoptiles, the corresponding suppression of the amyloplast development was also observed. Similar results were obtained in Arabidopsis hypocotyls. Thus, the induction of automorphogenesis and a concomitant decrease in graviresponsiveness occurred in plant shoots grown under microgravity conditions.     

A theoretical assessment of the feasibility of potential Lunar Reconnaissance Orbiter radio occultation observations of the lunar ionosphere

The existence of a “dense” lunar ionosphere has been controversial for decades. Positive ions produced from the lunar surface and exosphere are inferred to have densities that are $?$${10}^6$ – ${10}^7$ m^?3 near the surface and smaller at higher altitudes, yet electron densities derived from radio occultation measurements occasionally exceed these values by orders of magnitude. For example, about 4% of the single-spacecraft radio occultation measurements from Kaguya/SELENE were consistent with peak electron densities of $~$$3\times {10}^8$ m^?3. Space plasmas should be neutral on macroscopic scales, so this represents a substantial discrepancy. Aditional observations of electron densities in the lunar ionosphere are critical to resolving this longstanding paradox. Here we theoretically assess whether radio occultation observations using two-way coherent S-band radio signals from the Lunar Reconnaissance Orbiter (LRO) spacecraft could provide useful measurements of electron densities in the lunar ionosphere. We predict the uncertainty in a single LRO radio occultation measurement of electron density to be $~$$3\times {10}^8$ m^?3, comparable to occasional observations by Kaguya/SELENE of a dense lunar ionosphere. Thus an individual profile from LRO is unlikely to reliably detect the lunar ionosphere; however, averages of multiple ($~$10) LRO profiles acquired under similar geophysical and viewing conditions should be able to make reliable detections. An observing rate of six ingress occultations per day ($~$2000 per year) could be achieved with minimal impact on current LRO operations. This rate compares favorably with the 378 observations reported from the single-spacecraft experiment on Kaguya/SELENE between November 2007 and June 2009. The large number of observations possible for LRO would be sufficient to permit wide-ranging investigations of spatial and temporal variations in the poorly understood lunar ionosphere. These findings strengthen efforts to conduct such observations with LRO.     

Planet-B mission to Mars - 1998

PLANET-B is the Japanese Mars orbiter program. The primary objective of the program is to study the Martian aeronomy, putting emphasis on the interaction of the Martian upper atmosphere with the solar wind. The launch of the spacecraft is scheduled for August, 1998. The periapsis altitude and the apoapsis are 150 km and 15 Mars radii, respectively. The dry weight of the orbiter is 186 kg including 14 science instruments. Advanced technologies are employed in the design of the spacecraft in order to overcome the weight limitation. This paper describes the scientific objectives of the PLANET-B program and outline of the spacecraft system.     

Development of data base of the ionosphere disturbances

A properly weighted measure of histogram distortion is proposed to evaluate data with different time scales for any index in any field. This approach is applied to produce a long-term data base of disturbance measure of the geomagnetic indices and ionospheric VI sounding characteristics. It is shown that the ionosphere disturbances detected by the proposed technique include all the storm periods defined by the statistical procedure used earlier at IRPL, NBS, Washington, D.C.     

Modeling of “gradual” solar energeticparticle events using a stochastic differential equation method

We have modeled “gradual” solar energetic particle events through numerical simulations using a StochasticDifferential Equation (SDE) method. We consider that energetic particle events are roughly divided into two groups: (1) where the shock was driven by coronal mass ejections (CMEs) associated with large solar flares, and (2) where they have no related solar events apart from the CMEs. (The detailed classification of energetic particle events was discussed in our previous paper.) What we call “gradual” solar energetic particle events belong to the former group. Particles with energies greater than 10 MeV are observed within several hours after the occurrence of flares and CMEs in many gradual events. By applying the SDE method coupled with particle splitting to diffusive acceleration, we found that an injection of high energy particles is necessary for early enhancement of such a high-energy proton flux and that it should not be presumed that the solar wind particles act as the seed population.     

Formation of amino acid precursors in cometary ice environments by cosmic radiation.

Cometary ices are believed to contain water, carbon monoxide, methane and ammonia, and are possible sites for the formation and preservation of organic compounds relating to the origin of life. Cosmic rays, together with ultraviolet light, are among the most effective energy sources for the formation of organic compounds in space. In order to study the possibility of the formation of amino acids in comets or their precursory bodies (interstellar dust grains), several types of ice mixtures made in a cryostat at 10 K ("simulated cometary ices") were irradiated with high energy protons. After irradiation, the volatile products were analyzed with a quadrupole mass spectrometer, while temperature of the cryostat was raised to room temperature. The non-volatile products remaining in the cryostat at room temperature were collected with water. They were acid-hydrolyzed, and analyzed by ion-exchange chromatography. When an ice mixture of carbon monoxide (or methane), ammonia and water was irradiated, some hydrocarbons were formed, and amino acids such as glycine and alanine were detected in the hydrolyzate. These results suggest the possible formation of "amino acid precursors" (compounds yielding amino acids after hydrolysis) in interstellar dust grains by cosmic radiation. We previously reported that amino acid precursors were formed when simulated primitive planetary atmospheres were irradiated with cosmic ray particles. It will be of great interest to compare the amount of bioorganic compounds that were formed in the primitive earth and that brought by comets to the earth.     

Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Biosphere 2 Test Module: a ground-based sunlight-driven prototype of a closed ecological life support system.

Constructed in 1986, the Biosphere 2 Test Module has been used since the end of that year for closed ecological systems experiments. It is the largest closed ecological facility ever built, with a sealed variable volume of some 480 cubic meters. It is built with a skin of steel spaceframes with double-laminated glass panels admitting about 65 percent Photosynthetically Active Radiation (PAR). The floor is of welded steel and there is an underground atmospheric connection via an air duct to a variable volume chamber ("lung") permitting expansion and contraction of the Test Module's air volume caused by changes in temperature and barometric pressure, which causes a slight positive pressure from inside the closed system to the outside thereby insuring that the very small leakage rate is outward. Several series of closed ecological system investigations have been carried out in this facility. One series of experiments investigated the dynamics of higher plants and associated soils with the atmosphere under varying light and temperature conditions. Another series of experiments included one human in the closed system for three, five and twenty-one days. During these experiments the Test Module had subsystems which completely recycled its water and atmosphere; all the human dietary needs were produced within the facility, and all wastes were recycled using a marsh plant/microbe system. Other experiments have examined the capability of individual component systems used, such as the soil bed reactors, to eliminate experimentally introduced trace gases. Analytic systems developed for these experiments include continuous monitors of eleven atmospheric gases in addition to the complete gas chromatography mass spectrometry (GCMS) examinations of potable, waste system and irrigation water quality.     

Simple solutions for hyperbolic and related position fixes

Navigation fixed from range differences to three stations and an additional piece of information are investigated. It is shown that if the additional information is the navigator altitude, or the range difference to a fourth station, the computation of the navigation fix is reduced to finding the roots of a quadratic. If the additional information is the range to another station, or that the navigator is on the Earth ellipsoid, the fix can be obtained by solving a quartic. By emphasizing the underlying geometric interpretations, these fixes and their simple solutions are made clear. The derivations also show that the same solution algorithms are applicable if the basic navigation measurements are range sums instead of range differences