Spaceflight results in increase of thick filament but not thin filament proteins in the paramyosin mutant of Caenorhabditis elegans

We have investigated the effect of microgravity during spaceflight on body-wall muscle fiber size and muscle proteins in the paramyosin mutant of Caenorhabditis elegans. Both mutant and wild-type strains were subjected to 10 days of microgravity during spaceflight and compared to ground control groups. No significant change in muscle fiber size or quantity of the protein was observed in wild-type worms; where as atrophy of body-wall muscle and an increase in thick filament proteins were observed in the paramyosin mutant unc-15(e73) animals after spaceflight. We conclude that the mutant with abnormal muscle responded to microgravity by increasing the total amount of muscle protein in order to compensate for the loss of muscle function.     

The effects of magnetic fields on period changes,mass transfer and evolution of algol binaries

Variations in the magnetic pressure and flux blocking by starspots during the magnetic cycle of the cool semidetached component of an Algol binary may cause cyclic changes in the quadrupole moment and moment of inertia of the star which can cause alternate period changes. Since several different processes and timescales are involved, the orbital period changes may not correlate strongly with the indicators of magnetic activity. The structural changes in the semidetached component can also modulate the mass transfer rate. Sub-Keplerian velocities, supersonic turbulence, and high temperature regions in circumstellar material around the accreting star may all be a consequence of magnetic fields embedded in the flow. Models for the evolution of Algols which include the effects of angular momentum loss (AML) through a magnetized wind may have underestimated the AML rate by basing it on results from main sequence stars. Evolved stars appear to have higher AML rates, and there may be additional AML in a wind from the accretion disk.     

The Theory of a Piezoelectric Disc Gyroscope

The theory of a new type of vibratory rate gyroscope is considered. The design considered is based upon a disc of piezoelectric material on which are deposited drive and pick-off electrodes. The equations of motion of the disc are derived and the response of the gyroscope to constant and harmonic rates of turn determined.     

Optimised radiative cooling of infrared space telescopes and applications to possible missions

General principles are outlined for the design of space infrared telescopes intended to cool by radiation to the lowest temperatures attainable without the use of on-board cryogens, and assuming on-orbit cooling after a warm launch. Maximum protection from solar and earth heating, maximum radiating area and efficiency and minimum absorbing area and absorptivity are the obvious basic criteria. The optimised design is a short, fat telescope surrounded by a series of radiation shields, each cooled by its own radiator. Maximising the longitudinal conductivity of the radiation shields and of the telescope tube itself is important both to the on-orbit cooling time and the final achieveable temperature. Realistic designs take between 80 and 200 days to cool to within a few degrees of equilibrium temperatures, depending on the materials used. Great advantages accrue from the use of an orbit distant from earth. Both simple models and detailed simulations suggest that temperatures of 30 to 40 K are attainable in high earth orbits. Placing a radiatively cooled telescope in a halo orbit around the Lagrangian point L2 is a particularly attractive option and significantly lower temperatures can be achieved there than in Earth orbit. Optimised radiative cooling is an important element of the small Japanese mission SMIRT. We suggest that a combination of an ESA Medium-sized Mission with a NASA Explorer to send a 2m+ telescope to an L2 halo orbit would provide a cost-effective and powerful long-duration facility for the early 21st century.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Generic training for ATE technicians

It is argued that the job of an automatic test equipment (ATE) technician is evolving toward that of a computer operator. The technician has automatic tools (test programs) written for every replaceable unit in his test set. The technician selects which test is run. During the test the technician is presented results and given options if a test fails. If the option called out by the test program does not fix the problem, the technician must go back into his tool box (his library of test programs) and select the right tool to do the job. This is true for a technician working on any piece of ATE. It is contended that training for these technicians should be centered around troubleshooting skills with initially less emphasis on the hardware implementation details. These skills could be developed with a genetic trainer     

Rhythmic biological systems under microgravity conditions.

Rhythmic phenomena in biology cover a wide frequency spectrum. In Space, the rhythms will encounter microgravity conditions which can, therefore, be a valuable tool for their understanding. A review and discussion of important effects of gravity/absence of gravity on biological systems will be given. Convection will be emphasized as a mechanism which is drastically reduced in Space. Microgravity might also affect the coupling between individual oscillators in a multi-oscillatory system. The environmental interference with rhythms will be discussed with a simple feedback as a starting point. Model simulations will be presented and clinostat and microgravity-conditions will be discussed in a specific case, viz. the gravitropical system of plants which can show sustained oscillations.     

Fast Maximnurm Likelihood Joint Estimation of Frequency and Frequency Rate

A fast maximum likelihood algorithm is presented that jointly estimates the frequency and frequency rate of a sinusoid corrupted by additive Gaussian white noise. It consists of a coarse search and a fine search. First the two-dimensional frequency-frequency rate plane is subdivided into parallelograms whose size depends on the region of convergence of Newton's method used in maximizing the log-likelihood function (LLF). The size of the parallelogram is explicitly computed and is optimal for the method used. The coarse search consists of maximizing the LLF over the vertices of the parallelograms. Then starting at the vertex where the LLF attained its maximum, a two-dimensional Newton's method to find the absolute maximum of the LLF is implemented. This last step consists of the fine search. The rate of convergence of Newton's method is cubic, and is extremely fast. Furthermore Newton's method will converge after two iterations when the starting point used in the method lies within 75 percent of the distances defined by the parallelogram of convergence whose center coincides with the true values of frequency and frequency rate. In this case, the root mean square error (RMSEs) for frequency and frequency rate are practically equal to the Cramer-Rao bound at all signal-to-noise ratio (SNR)?15 dB. The frequency-frequency rate ambiguity function is shown to be even and its periodicities are extracted.     

DNA-lesion and cell death by alpha-particles and nitrogen ions.

When the natural logarithm of the surviving fraction is plotted against the dose of radiation, curves with shoulders at relatively high survival levels are obtained after gamma-rays. The curves were practically linear in case of HMV-I and HA-1 cells irradiated by charged particle beams. These cells were derived from human malignant melanoma and Chinese hamster cells, respectively. The amount of DNA single strand breaks (ssb) by gamma-rays or nitrogen-ions (LET=530KeV/micrometers) in HMV-I cells increases linearly with increment in dose, when the ssb is detected using the alkaline elution technique. There is no close relationship between the dose-response curve of the ssb and the dose-survival curves after gamma-rays or N-ions. The amount of DNA double strand breaks (dsb) by gamma-rays increases quadratically with increment of dose, in both HMV-I cells and HA-1 cells, when the dsb is detected using the neutral elution technique. The survival fraction for HA-1 cells is slightly higher than that for HMV-I cells, at the same dose, and the amount of dsb for HA-1 cells is considerably greater than that for HMV-I cells. These results suggest that the radiosensitivities to gamma-rays in different cell lines do not correspond to the number of DNA strand breaks. The amount of both non-repairable ssb and dsb also increases quadratically with increment of dose for gamma-rays and almost linearly with increment of dose for N-ions and alpha-particles (LET=36keV/micrometers for HA-1 cells and LET=77keV/micrometers for HMV-I cells). The dose-response curves for non-repairable dsb in case of these radiations seemed to mirror image the dose-survival curves for these radiations, in both cell lines. The number of non-repairable DNA strand breaks in the two cell lines, at the same level of survival was much the same. These results show the close relationship between the induction of non-repairable DNA strand breaks and cell killing.