Prebiotic oligodeoxynucleotide synthesis in a cyclic evaporating system at low temperatures.

Deoxynucleoside 5'-monophosphates were condensed by cyanamide or by l-ethyl-3-(3-dimethylaminopropyl) carbodiimide in the presence of ammonium chloride at 0 degree, 37 degrees or 60 degrees C through several cycles of evaporation to dryness with replenishment of all reactants at each cycle. We found that at 37 degrees or 60 degrees cyanamide gives distinctly more high molecular weight material than does carbodiimide. Indeed, the yield of condensed products for the cyanamide reaction (dimers and higher oligomers) was found to be between 60 percent and 80 percent. The molecular weight distribution of the product shifts to higher molecular weights as cycling continues at 37 degrees or 60 degrees for both condensing agents. The water soluble carbodiimide gives higher yields of low molecular weight product but much lower yields of the higher molecular weight products. At 0 degree yields of high molecular weight product were low for both condensing agents. Results of characterization of the products demonstrate the synthesis of oligodeoxynucleotides including tetramers, with 3'-5' phosphodiester linkages.     

Functional state of chromatin and proliferative activity of meristematic cells in pea seedlings under various clinostatic conditions.

Data from a complex cytochemical analysis show that the functional state of chromatin and the level of the cell proliferative activity may be reliable cytological criteria for primary structural and functional changes that result in disturbances of plant growth and development. Autoradiographic and cytophotonetric studies made it possible to establish certain differences, induced by fast rotation (50 rev/min) on the clinostat, in the chromatin state and cell reproduction of the pea seedling root meristem for the initial stages of plant development. There were no essential differences for the given parameters under slow (2 rev/min) clinostatic conditions.     

Evaluation of light emitting diode characteristics for a space-based plant irradiation source.

Light emitting diodes (LEDs) are a promising irradiation source for plant growth in space. Improved semiconductor technology has yielded LED devices fabricated with gallium aluminum arsenide (GaAlAs) chips which have a high efficiency for converting electrical energy to photosynthetically active radiation. Specific GaAlAs LEDs are available that emit radiation with a peak wavelength near the spectral peak of maximum quantum action for photosynthesis. The electrical conversion efficiency of installed systems (micromole s-1 of photosynthetic photons per watt) of high output LEDs can be within 10% of that for high pressure sodium lamps. Output of individual LEDs were found to vary by as much as 55% from the average of the lot. LED ratings, in mcd (luminous intensity per solid angle), were found to be proportional to total photon output only for devices with the same dispersion angle and spectral peak. Increasing current through the LED increased output but also increased temperature with a consequent decrease in electrical conversion efficiency. A photosynthetic photon flux as high as 900 micromoles m-2 s-1 has been produced on surfaces using arrays with LEDs mounted 7.6 mm apart, operating as a current of 50 mA device-1 and at an installed density of approximately 17,200 lamps m-2 of irradiated area. Advantages of LEDs over other electric light sources for use in space systems include long life, minimal mass and volume and being a solid state device.     

An advanced flight control and navigation system implementation fortactical helicopters

US Army helicopters that will allow single-crewmember operability in tactical environments require a high degree of automation. A tightly integrated flight control and navigation system has been studied. An architecture is discussed which meets anticipated flight control and navigation requirements, at an acceptable weight, using techniques of functional and physical integration. The architecture maximizes the sharing of sensor and computational resources in a flight critical environment and uses modular equipment packaging, dual fault-tolerant tetrad ring-laser-gyro inertial measurement units, and triplex self-checking processor pair-based flight critical processing channels     

On ionospheric aerodynamics

This paper presents theoretical methods to determine the gas dynamic and the electrostatic effects due to the interaction caused by a rapidly moving body in the ionosphere. The principles of the methods are derived from the kinetic theory of collision-free plasma. It is shown that the collective behavior of the collision-free plasma makes it possible to use the fluid approach to treat the problems of ionospheric aerodynamics. Various solutions to the system of fluid and field equations that have direct bearing on the ionospheric aerodynamics are presented and discussed. Physical significances of the mathematical results are stressed. Some outstanding unsolved problems in ionospheric aerodynamics are elaborated and discussed.     

Thermoluminescent dose measurements on-board Salyut type orbital stations.

A small, vibration- and shock-resistant thermoluminescent dosemeter /TLD/ system--named PILLE--was developed at the Health Physics Department of the Central Research Institute for Physics, Budapest, to measure the cosmic radiation dose on board orbital stations. The first on-board measurements with this system were performed /by B. Farkas, the Hungarian astronaut/, on the Salyut-6 space station in 1980. The same instrument was used by other crews in the following years. Doses measured at different sites in Salyut-6 are presented. The dose rates varied from 0.07 to 0.11 mGy.day-1. After the first cosmic measurements, the system was further developed. The minimum detectable dose of the new TLD system is 1 microGy, i.e. less by on order of magnitude than that of the former system. The self-irradiation dose rate of the TLD bulbs is also reduced--by more than one order of magnitude--to 10 nGy.h-1, by the use of potassium-free glass for the bulb envelope. This new type of PILLE TLD system is currently on-board Salyut-7. The dose rates /0.12-0.23 mGy.day-1/ measured in 1983 are presented in detail.     

Potential defense applications of expert systems

The authors provide an overview of expert systems and how they may effect the development of future defense applications. Military uses of computers are outlined, and expert-systems fundamentals are described. Artificial research and development efforts by the military are examined, and potential military applications are discussed. Expert systems efforts at NASA, by the US Air Force, and for the Strategic Defence Initiative are considered     

Preliminary data of the thermal regime and circulation in the upper mesosphere and mesopause

Circulation and thermal structure for the 90–95 km region is described on the basis of the joint analysis of direct and indirect sounding data. Mean season charts of the 0.001 mb surface were compiled using data from meteor trials, ionospheric drifts, rocket launchings and data of the rotational temperature of hydroxyl emission.     

Some aspects of the application of the bunch of MPD engines

In the present work, the asymptotic dependence of the reservation multiplicity on the failure danger diminution coefficient in both cases of reservation replacement and continually acting reserver is investigated. The comparison of the method of reservation for different multiplicities with the method of diminution of the failure intensity, related to the mean performance time shows that the decrease of the failure intensity of the plazma engine is the most rational. Nevertheless, there is a value of the time flight for which even a simple duplication is better than arbitrarily large, though finite, failure intensity decrease of the engine. The reliability of coupled MOD-thrusters is verified experimentally. The specific character of the thruster V-I curves and, in particular, their increase in the pubricrcrisis region, yield normally working couples, conformly to many types of feed sources with weakly decreasing of constant V-I characteristics. The parallel connection of a second thruster is shown to double the customed current and, as a consequence the thruster intermediary regimes of the thruster and the dynamics of switching on and by breaking the electric line and stopping the propellant rate flow are investigated.     

Telerobotics: problems and research needs

With major emphasis on simulation, a university laboratory telerobotics facility permits problems to be approached by groups of graduate students. Helmet-mounded displays provide realism; the slaving of the display to the human operator's viewpoint gives a sense of `telepresence' that may be useful for prolonged tasks. Using top-down 3-D model control of distant images allows distant images to be reduced to a few parameters to update the model used for display to the human operator in a preview model to circumvent, in part, the communication delay. Also, the model can be used as a format for supervisory control and permit short-term local autonomous operations. Image processing algorithms can be made simpler and faster without trying to construct sensible images from the bottom. Control studies of telerobots lead to preferential manual control modes and, in this university environment, to basic paradigms for human motion and thence, perhaps, to redesign of robotic control, trajectory path planning, and rehabilitation prosthetics. Speculation as to future industrial drives for this telerobotic field suggests efficient roles for government agencies such as NASA