Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.     

Non-LTE analysis of a new pulsating PG 1159-type central star detected in the ROSAT XRT all sky survey

We summarize the analysis of a new PG 1159 star, i.e., a hydrogen deficient pre-white dwarf detected in the ROSAT XRT all sky survey /1/. The X-ray source RX J2117.1+3412 is relatively faint (≈0.33 cnt s⁻¹) and was selected for optical identification on the basis of its extreme X-ray softness. With V = 13.2, the counterpart of the X-ray source is in the optical the second brightest member of the PG1159 class. CCD imagery reveals that the star is surrounded by an old highly excited planetary nebula of faint surface brightness. Optical line profiles of HeII, CIV, and OVI and the overall optical and ROSAT PSPC (0.1–2.4 keV) energy distributions are compared to predictions of NLTE model atmospheres. We find an excellent agreement between the atmospheric parameters derived from optical and soft X-ray data. The effective temperature of the star is extremely high (150,000 K). Abundances of He, C and O are found to be consistent with those derived in other PG1159 stars whereas the surface gravity is significantly smaller in RXJ2117.1+3412. New optical spectra presented here show the presence of ultra-high excitation lines of O VIII.     

Error Covariance Analysis of a Karhunen-Loeve Collocation Estimator

An error covariance analysis of a two-dimensional gravity compensation technique (KLC) employing a Karhunen-Loeve gravity disturbance model and the linear least-square collocation algorithm for its estimation is presented, without actually using any data. Its performance is compared with another gravity compensation technique (KLE), whose error covariance analysis was previously presented by Gupta. From the mismodeling analysis, KLC appears to be superior to KLE.     

Early and late damages induced by heavy charged particle irradiation in embryonic tissue of Arabidopsis seeds.

Early and late effects of accelerated heavy ions (HZE) on the embryonic tissue of Arabidopsis thaliana seeds were investigated seeing that initial cells of the plant eumeristems resemble the original cells of animal and human tissues with continuous cell proliferation. The endpoints measured were lethality and tumorization in the M1-generation for early effects and embryonic lethality in the M2-generation for late effects. The biological endpoints are plotted as functions of the physical parameters of the irradiation i.e. ion fluence (p/cm2), dose (Gray), charge Z and linear energy transfer (LET). The results presented contribute to the estimation of the principles of biological HZE effects and thus may help to develop a unified theory which could explain the whole sequence from physical and chemical reactions to biological responses connected with heavy ion radiation. Additionally, the data of this paper may be used for the discussion of the quality factor for heavy ion irradiation needed for space missions and for HZE-application in radio-therapy by use of accelerators (UNILAC, (SIS/ESR), BEVALAC).     

Observation of enhanced sub-iron (Sc---Cr) to iron ration in low energy cosmic rays of 50–100 MeV/N in spacelab-3

Relative abundances of sub-iron (Sc-Cr) to iron nuclei in low energy (50–100 MeV/N) galactic cosmic rays have been determined from an analysis of about 100 events of heavy ions (Z = 10−28) recorded in a detector assembly flown in the Anuradha cosmic ray experiment in the Spacelab-3 on a six day mission in April–May 1985. The measured abundance ratio of (Sc-Cr)/Fe nuclei in 50–100 MeV/N energy range is 1.1 ± 0.3, and the present result of enhanced ratio of sub-iron to iron nuclei is in agreement with other experimental results in 200–800 MeV/N range. The over-abundance of iron secondaries at these low energies cannot be explained in the conventional models for propagation of cosmic rays. Available experimental data indicate a very different time history for the low energy iron-group, as compared to those of lighter nuclei in galactic cosmic rays.     

Scan conversion of radar images

The object of scan conversion is to resample a polar-sampled image into a Cartesian format while retaining integrity of the geometry and content of the image. Linear interpolation and decimation in two dimensions in the context of scan conversion are reviewed. Appropriate approximations of the nonlinear relationship between Cartesian and polar coordinates are then made to allow the application of the linear interpolation theory to produce a number of practical scan conversion algorithms. Together with an image prefiltering process, which is applied to areas which require scan conversion decimation, an effective scheme is presented     

Mammalian cell cultivation in space.

Equipment used in space for the cultivation of mammalian cells does not meet the usual standard of earth bound bioreactors. Thus, the development of a space worthy bioreactor is mandatory for two reasons: First, to investigate the effect on single cells of the space environment in general and microgravity conditions in particular, and second, to provide researchers on long term missions and the Space Station with cell material. However, expertise for this venture is not at hand. A small and simple device for animal cell culture experiments aboard Spacelab (Dynamic Cell Culture System; DCCS) was developed. It provides 2 cell culture chambers, one is operated as a batch system, the other one as a perfusion system. The cell chambers have a volume of 200 microliters. Medium exchange is achieved with an automatic osmotic pump. The system is neither mechanically stirred nor equipped with sensors. Oxygen for cell growth is provided by a gas chamber that is adjacent to the cell chambers. The oxygen gradient produced by the growing cells serves to maintain the oxygen influx by diffusion. Hamster kidney cells growing on microcarriers were used to test the biological performance of the DCCS. On ground tests suggest that this system is feasible.     

DNA-lesion and cell death by alpha-particles and nitrogen ions.

When the natural logarithm of the surviving fraction is plotted against the dose of radiation, curves with shoulders at relatively high survival levels are obtained after gamma-rays. The curves were practically linear in case of HMV-I and HA-1 cells irradiated by charged particle beams. These cells were derived from human malignant melanoma and Chinese hamster cells, respectively. The amount of DNA single strand breaks (ssb) by gamma-rays or nitrogen-ions (LET=530KeV/micrometers) in HMV-I cells increases linearly with increment in dose, when the ssb is detected using the alkaline elution technique. There is no close relationship between the dose-response curve of the ssb and the dose-survival curves after gamma-rays or N-ions. The amount of DNA double strand breaks (dsb) by gamma-rays increases quadratically with increment of dose, in both HMV-I cells and HA-1 cells, when the dsb is detected using the neutral elution technique. The survival fraction for HA-1 cells is slightly higher than that for HMV-I cells, at the same dose, and the amount of dsb for HA-1 cells is considerably greater than that for HMV-I cells. These results suggest that the radiosensitivities to gamma-rays in different cell lines do not correspond to the number of DNA strand breaks. The amount of both non-repairable ssb and dsb also increases quadratically with increment of dose for gamma-rays and almost linearly with increment of dose for N-ions and alpha-particles (LET=36keV/micrometers for HA-1 cells and LET=77keV/micrometers for HMV-I cells). The dose-response curves for non-repairable dsb in case of these radiations seemed to mirror image the dose-survival curves for these radiations, in both cell lines. The number of non-repairable DNA strand breaks in the two cell lines, at the same level of survival was much the same. These results show the close relationship between the induction of non-repairable DNA strand breaks and cell killing.     

Costas arrays with small number of cross-coincidences

A simple method is described for obtaining two or more Costas arrays with a maximum of one mutual bit or cross-coincidence, assuming that the vertical components of their relative shifts are appropriately bounded. The result can be useful for designing multiple Costas waveforms with small cross-ambiguities, if the potential Doppler shifts are restricted to a small portion of the waveform bandwidth