System identification of dynamic closed-loop control of total peripheral resistance by arterial and cardiopulmonary baroreceptors.

Prolonged exposure to microgravity in space flight missions (days) impairs the mechanisms responsible for defense of arterial blood pressure (ABP) and cardiac output (CO) against orthostatic stress in the post-flight period. The mechanisms responsible for the observed orthostatic intolerance are not yet completely understood. Additionally, effective counter measures to attenuate this pathophysiological response are not available. The aim of this study was to investigate the ability of our proposed system identification method to predict closed-loop dynamic changes in TPR induced by changes in mean arterial pressure (MAP) and right atrial pressure (RAP). For this purpose we designed and employed a novel experimental animal model for the examination of arterial and cardiopulmonary baroreceptors in the dynamic closed-loop control of total peripheral resistance (TPR), and applied system identification to the analysis of beat-to-beat fluctuations in the measured signals. Grant numbers: NAG5-4989.     

Location and sampling of aqueous and hydrothermal deposits in martian impact craters

Do large craters on Mars represent sites that contain aqueous and hydrothermal deposits that provide clues to astrobiological processes? Are these materials available for sampling in large craters? Several lines of evidence strongly support the exploration of large impact craters to study deposits important for astrobiology. The great depth of impact craters, up to several kilometers relative to the surrounding terrain, can allow the breaching of local aquifers, providing a source of water for lakes and hydrothermal systems. Craters can also be filled with water from outflow channels and valley networks to form large lakes with accompanying sedimentation. Impact melt and uplifted basement heat sources in craters > 50 km in diameter should be sufficient to drive substantial hydrothermal activity and keep crater lakes from freezing for thousands of years, even under cold climatic conditions. Fluid flow in hydrothermal systems is focused at the edges of large planar impact melt sheets, suggesting that the edge of the melt sheets will have experienced substantial hydrothermal alteration and mineral deposition. Hydrothermal deposits, fine-grained lacustrine sediments, and playa evaporite deposits may preserve evidence for biogeochemical processes that occurred in the aquifers and craters. Therefore, large craters may represent giant Petri dishes for culturing preexisting life on Mars and promoting biogeochemical processes. Landing sites must be identified in craters where access to the buried lacustrine sediments and impact melt deposits is provided by processes such as erosion from outflow channels, faulting, aeolian erosion, or excavation by later superimposed cratering events. Very recent gully formation and small impacts within craters may allow surface sampling of organic materials exposed only recently to the harsh oxidizing surface environment.     

Mineralogical biosignatures and the search for life on Mars

If life ever existed, or still exists, on Mars, its record is likely to be found in minerals formed by, or in association with, microorganisms. An important concept regarding interpretation of the mineralogical record for evidence of life is that, broadly defined, life perturbs disequilibria that arise due to kinetic barriers and can impart unexpected structure to an abiotic system. Many features of minerals and mineral assemblages may serve as biosignatures even if life does not have a familiar terrestrial chemical basis. Biological impacts on minerals and mineral assemblages may be direct or indirect. Crystalline or amorphous biominerals, an important category of mineralogical biosignatures, precipitate under direct cellular control as part of the life cycle of the organism (shells, tests, phytoliths) or indirectly when cell surface layers provide sites for heterogeneous nucleation. Biominerals also form indirectly as by-products of metabolism due to changing mineral solubility. Mineralogical biosignatures include distinctive mineral surface structures or chemistry that arise when dissolution and/or crystal growth kinetics are influenced by metabolic by-products. Mineral assemblages themselves may be diagnostic of the prior activity of organisms where barriers to precipitation or dissolution of specific phases have been overcome. Critical to resolving the question of whether life exists, or existed, on Mars is knowing how to distinguish biologically induced structure and organization patterns from inorganic phenomena and inorganic self-organization. This task assumes special significance when it is acknowledged that the majority of, and perhaps the only, material to be returned from Mars will be mineralogical.     

Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system

The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants--SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances--products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover.     

Promoting research and education in basic space science: the approach of the UN/ESA workshops

UN-affiliated regional centres for space science and technology education are being established or are in operation in Africa (Morocco, Nigeria), Asia and the Pacific (India), Latin America and the Caribbean (Brazil, Mexico), and Western Asia (Jordan). Education curricula at the university level, embracing remote sensing, satellite communications, satellite meteorology, and space science have been developed for these centres. This article briefly reports on the structure of the most recent updated education curricula in the four disciplines that have been made available for implementation in 2002 and 2003, in the six official languages of the United Nations. This is also an effort to bridge the gap between such education curricula as they vary significantly between nations and among educational institutions in nations.     

Finding extraterrestrial organisms living on thermosynthesis

During thermal cycling, organisms could live on thermosynthesis, a theoretical mechanism applicable to the origin of life and the early evolution of biological energy conversion. All extraterrestrial ice may be a repository for frozen dead or dormant organisms from earlier stages of evolution. In the presence of a thermal gradient within the ice, organisms might still be harvesting energy from thermosynthesis. Possible habitats for thermosynthesizers can be found throughout the Solar System, particularly in the cold traps on Mercury and the Moon, convecting waters on Mars, the oceans on moons in the outer Solar System, and smaller bodies rotating in the sunlight such as cosmic dust, meteorites, asteroids, and comets. A general strategy for detecting thermosynthetic organisms on Earth is offered, and highlights of current and upcoming robotic exploratory missions relevant to the detection of thermosynthesis are reviewed.     

Structural health management technologies for inflatable/deployable structures: Integrating sensing and self-healing

Inflatable/deployable structures are under consideration as habitats for future Lunar surface science operations. The use of non-traditional structural materials combined with the need to maintain a safe working environment for extended periods in a harsh environment has led to the consideration of an integrated structural health management system for future habitats, to ensure their integrity. This article describes recent efforts to develop prototype sensing technologies and new self-healing materials that address the unique requirements of habitats comprised mainly of soft goods. A new approach to detecting impact damage is discussed, using addressable flexible capacitive sensing elements and thin film electronics in a matrixed array. Also, the use of passive wireless sensor tags for distributed sensing is discussed, wherein the need for on-board power through batteries or hardwired interconnects is eliminated. Finally, the development of a novel, microencapuslated self-healing elastomer with applications for inflatable/deployable habitats is reviewed.     

With a grain of salt: what halite has to offer to discussions on the origin of life

This experimental study investigated how the dynamics of the crystallization of the evaporite mineral halite could affect the accumulation and preservation of organic macromolecules present in the crystallizing solution. Halite was grown under controlled conditions in the presence of polymer nanoparticles that acted as an analog to protocellular material. Optical microscopy, atomic force microscopy, and laser scanning confocal fluorescence microscopy were used to trace the localization of the nanoparticles during and after growth of halite crystals. The present study revealed that the organic nanoparticles were not regularly incorporated within the halite, but were very concentrated on its surfaces. Their distribution was controlled dominantly by the morphologic surface features of the mineral rather than by specific molecular interactions with an atomic plane of the mineral. This means that the distribution of organic molecules was controlled by surfaces like those of halite's evaporitic growth forms. The experiments with halite also demonstrated that a mineral need not continuously incorporate organic molecules during its crystallization to preserve those molecules: After rejection by (non-incorporation into) the crystallizing halite, the organic nanoparticles increased in concentration in the evaporating brine. They ultimately either adsorbed in rectilinear patterns onto the hopper-enhanced surfaces and along discontinuities within the crystals, or they were encapsulated within fluid inclusions. Of additional importance in origin-of-life considerations is the fact that halite in the natural environment rapidly can change its role from that of a protective repository (in the absence of water) to that of a source of organic particles (as soon as water is present) when the mineral dissolves.     

Autonomic function testing aboard the ISS using “PNEUMOCARD”

Investigations of blood pressure, heart rate (HR), and heart rate variability (HRV) during long term space flights on board the “ISS” have shown characteristic changes of autonomic cardiovascular control. Therefore, alterations of the autonomic nervous system occurring during spaceflight may be responsible for in- and post-flight disturbances. The device “Pneumocard” was developed to further investigate autonomic cardiovascular and respiratory function aboard the ISS. The hard-software diagnostic complex “Pneumocard” was used during in-flight experiment aboard ISS for autonomic function testing. ECG, photoplethysmography, respiration, transthoracic bioimpedance and seismocardiography were assessed in one male cosmonaut (flight lengths six month). Recordings were made prior to the flight, late during flight, and post-flight during spontaneous respiration and controlled respiration at different rates.HR remained stable during flight. The values were comparable to supine measurements on earth. Respiratory frequency and blood pressure decreased during flight. Post flight HR and BP values increased compared to in-flight data exceeding pre-flight values. Cardiac time intervals did not change dramatically during flight. Pulse wave transit time decreased during flight. The maximum of the first time derivative of the impedance cardiogram, which is highly correlated with stroke volume was not reduced in-flight.Our results demonstrate that autonomic function testing aboard the ISS using “Pneumocard” is feasible and generates data of good quality. Despite the decrease in BP, pulse wave transit time was found reduced in space as shown earlier. However, cardiac output did not decrease profoundly in the investigated cosmonaut.Autonomic testing during space flight detects individual changes in cardiovascular control and may add important information to standard medical control. The recent plans to support a flight to Mars, makes these kinds of observations all the more relevant and compelling.