Modeled microgravity increases filamentation, biofilm formation, phenotypic switching, and antimicrobial resistance in Candida albicans

Candida albicans is an opportunistic fungal pathogen responsible for a variety of cutaneous and systemic human infections. Virulence of C. albicans increases upon exposure to some environmental stresses; therefore, we explored phenotypic responses of C. albicans following exposure to the environmental stress of low-shear modeled microgravity. Upon long-term (12-day) exposure to low-shear modeled microgravity, C. albicans transitioned from yeast to filamentous forms at a higher rate than observed under control conditions. Consistently, genes associated with cellular morphology were differentially expressed in a time-dependent manner. Biofilm communities, credited with enhanced resistance to environmental stress, formed in the modeled microgravity bioreactor and had a more complex structure than those formed in control conditions. In addition, cells exposed to low-shear modeled microgravity displayed phenotypic switching, observed as a near complete transition from smooth to "hyper" irregular wrinkle colony morphology. Consistent with the presence of biofilm communities and increased rates of phenotypic switching, cells exposed to modeled microgravity were significantly more resistant to the antifungal agent Amphotericin B. Together, these data indicate that C. albicans adapts to the environmental stress of low-shear modeled microgravity by demonstrating virulence-associated phenotypes.     

A Superlimiting Phased-Array Receiving System in a Two-Source Environment

The performance of a uniformly spaced phased-steered line array with element channel superlimiting is studied for far-field sources consisting of 1) two sinusoidal signals with different frequencies and angular locations, and 2) a sinusoid and a noise signal at different angular locations. Attention is focused on the nonlinear case where internal noise is negligible compared to both input signals. The analysis for the two-sinusoid case gives the precise frequencies, positions, and amplitudes of all apparent sources. In addition to the two active sources, the array output has an array of images arranged symmetrically in sine space about the larger input, at intervals equal to the spacing between the two active sources. For the case of a separated sinusoid and a noise source, the analysis shows that the angular positions and average powers of the array outputs duplicate the double-sinusoid results, but the images have noise-like spectra. The analyses are confirmed by experimental results obtained with a 60-element superlimiting X-band array.     

Instrument Boom Mechanisms on the THEMIS Satellites; Magnetometer, Radial Wire, and Axial Booms

The five “Time History of Events and Macroscale Interactions during Substorms” (THEMIS) micro-satellites launched on a common carrier by a Delta II, 7925 heavy, on February 17, 2007. This is the fifth launch in the NASA MeDIum class EXplorer (MIDEX) program. In the mission proposal the decision was made to have the University of California Berkeley Space Sciences Laboratory (UCB-SSL) mechanical engineering staff provide all of the spacecraft appendages, in order to meet the short development schedule, and to insure compatibility. This paper describes the systems engineering, design, development, testing, and on-orbit deployment of these boom systems that include: the 1 and 2 meter carbon fiber composite magnetometer booms, the 40 and 50 m tip to tip orthogonal spin-plane wire boom pairs, and the 6.3 m dipole stiff axial booms.