Preparation of the Biochip experiment on the EXPOSE-R2 mission outside the International Space Station

Biochips might be suited for planetary exploration. Indeed, they present great potential for the search for biomarkers – molecules that are the sign of past or present life in space – thanks to their size (miniaturized devices) and sensitivity. Their detection principle is based on the recognition of a target molecule by affinity receptors fixed on a solid surface. Consequently, one of the main concerns when developing such a system is the behavior of the biological receptors in a space environment. In this paper, we describe the preparation of an experiment planned to be part of the EXPOSE-R2 mission, which will be conducted on the EXPOSE-R facility, outside the International Space Station (ISS), in order to study the resistance of biochip models to space constraints (especially cosmic radiation and thermal cycling). This experiment overcomes the limits of ground tests which do not reproduce exactly the space parameters. Indeed, contrary to ground experiments where constraints are applied individually and in a limited time, the biochip models on the ISS will be exposed to cumulated constraints during several months. Finally, this ISS experiment is a necessary step towards planetary exploration as it will help assessing whether a biochip can be used for future exploration missions.     

The X/X/KA-band deep space transponder for the BepiColombo mission to mercury

This paper reports the main characteristics of the deep space transponder (DST) equipment that has been designed, developed and tested by Thales Alenia Space—Italy (TAS-I) for the European Space Agency (ESA) BepiColombo mission to Mercury.     

Analytical solutions for the relative motion of spacecraft subject to Lorentz-force perturbations

A spacecraft capable of producing higher-than-natural electrostatic charges may achieve propellantless orbital maneuvering via the Lorentz-force interaction with a planetary magnetic field. Development of maneuver strategies for these propellantless vehicles is complicated by the fact that the perturbative Lorentz force acts along only a single line of action at any instant. Relative-motion dynamical models are developed that lead to approximate analytical solutions for the motion of charged spacecraft subject to the Lorentz force. These solutions indicate that the principal effects of the Lorentz force on a spacecraft in a circular orbit are to change the intrack position and to change the orbit plane. A rendezvous example is presented in which a spacecraft with a specific charge of ?3.81 $\times$ 10^?4 C/kg reaches a target vehicle initially 10 km away (on the same equatorial low-Earth orbit) in 1 day. Fly-around maneuvers may be achieved in low-Earth orbit with specific charges on the order of 0.001 C/kg.     

A new universal equation for the time of transfer between two points of the central gravitational field

The paper presents the derivation of two new equations for calculating the time of transfer between two points of the central gravitational field: for hyperbolic orbits and the universal equation for elliptical and hyperbolic orbits. In the paper we have used as an independent variable, instead of the linear elements (semimajor axis, focal parameter of orbit or a chord connecting the ends of boundary radii of transfer), the angular parameter—the angle between the radius vector of an initial point of transfer and the vector of initial velocity of transfer. Paper’s material is a continuation of that presented in the “Space research“ Journal, vol. 2, March–April, 2009.     

Thyrotropin receptor and membrane interactions in FRTL-5 thyroid cell strain in microgravity

The aim of this work was to analyze the possible alteration of thyrotropin (TSH) receptors in microgravity, which could explain the absence of thyroid cell proliferation in the space environment. Several forms of the TSH receptor are localized on the plasma membrane associated with caveolae and lipid rafts. The TSH regulates the fluidity of the cell membrane and the presence of its receptors in microdomains that are rich in sphingomyelin and cholesterol. TSH also stimulates cyclic adenosine monophosphate (cAMP) accumulation and cell proliferation. Reported here are the results of an experiment in which the FRTL-5 thyroid cell line was exposed to microgravity during the Texus-44 mission (launched February 7, 2008, from Kiruna, Sweden). When the parabolic flight brought the sounding rocket to an altitude of 264?km, the culture media were injected with or without TSH in the different samples, and weightlessness prevailed on board for 6 minutes and 19 seconds. Control experiments were performed, in parallel, in an onboard 1g centrifuge and on the ground in Kiruna laboratory. Cell morphology and function were analyzed. Results show that in microgravity conditions the cells do not respond to TSH treatment and present an irregular shape with condensed chromatin, a modification of the cell membrane with shedding of the TSH receptor in the culture medium, and an increase of sphingomyelin-synthase and Bax proteins. It is possible that real microgravity induces a rearrangement of specific sections of the cell membrane, which act as platforms for molecular receptors, thus influencing thyroid cell function in astronauts during space missions.     

Adsorption of amino acids (ALA, CYS, HIS, MET) on zeolites: fourier transform infrared and Raman spectroscopy investigations

Minerals adsorb more amino acids with charged R-groups than amino acids with uncharged R-groups. Thus, the peptides that form from the condensation of amino acids on the surface of minerals should be composed of amino acid residues that are more charged than uncharged. However, most of the amino acids (74%) in today's proteins have an uncharged R-group. One mechanism with which to solve this paradox is the use of organophilic minerals such as zeolites. Over the range of pH (pH 2.66-4.50) used in these experiments, the R-group of histidine (His) is positively charged and neutral for alanine (Ala), cysteine (Cys), and methionine (Met). In acidic hydrothermal environments, the pH could be even lower than those used in this study. For the pH range studied, the zeolites were negatively charged, and the overall charge of all amino acids was positive. The conditions used here approximate those of prebiotic Earth. The most important finding of this work is that the relative concentrations of each amino acid (X=His, Met, Cys) to alanine (X/Ala) are close to 1.00. This is an important result with regard to prebiotic chemistry because it could be a solution for the paradox stated above. Pore size did not affect the adsorption of Cys and Met on zeolites, and the Si/Al ratio did not affect the adsorption of Cys, His, and Met. ZSM-5 could be used for the purification of Cys from other amino acids (Student-Newman-Keuls test, p<0.05), and mordenite could be used for separation of amino acids from each other (Student-Newman-Keuls test, p<0.05). As shown by Fourier transform infrared (FT-IR) spectra, Ala interacts with zeolites through the [Formula: see text] group, and methionine-zeolite interactions involve the COO, [Formula: see text], and CH(3) groups. FT-IR spectra show that the interaction between the zeolites and His is weak. Cys showed higher adsorption on all zeolites; however, the hydrophobic Van der Waals interaction between zeolites and Cys is too weak to produce any structural changes in the Cys groups (amine, carboxylic, sulfhydryl, etc.); thus, the FT-IR and Raman spectra are the same as those of solid Cys.     

Deducing Electron Properties from Hard X-ray Observations

X-radiation from energetic electrons is the prime diagnostic of flare-accelerated electrons. The observed X-ray flux (and polarization state) is fundamentally a convolution of the cross-section for the hard X-ray emission process(es) in question with the electron distribution function, which is in turn a function of energy, direction, spatial location and time. To address the problems of particle propagation and acceleration one needs to infer as much information as possible on this electron distribution function, through a deconvolution of this fundamental relationship. This review presents recent progress toward this goal using spectroscopic, imaging and polarization measurements, primarily from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI). Previous conclusions regarding the energy, angular (pitch angle) and spatial distributions of energetic electrons in solar flares are critically reviewed. We discuss the role and the observational evidence of several radiation processes: free-free electron-ion, free-free electron-electron, free-bound electron-ion, photoelectric absorption and Compton backscatter (albedo), using both spectroscopic and imaging techniques. This unprecedented quality of data allows for the first time inference of the angular distributions of the X-ray-emitting electrons and improved model-independent inference of electron energy spectra and emission measures of thermal plasma. Moreover, imaging spectroscopy has revealed hitherto unknown details of solar flare morphology and detailed spectroscopy of coronal, footpoint and extended sources in flaring regions. Additional attempts to measure hard X-ray polarization were not sufficient to put constraints on the degree of anisotropy of electrons, but point to the importance of obtaining good quality polarization data in the future.     

Improved rainfall estimation over the Indian region using satellite infrared technique

The GOES Precipitation Index (GPI) technique (Arkin, 1979) for rainfall estimation has been in operation for the last three decades. However, its applications are limited to the larger temporal and spatial scales. The present study focuses on the augmentation on GPI technique by incorporating a moisture factor for the environmental correction developed by Vicente et al. (1998). It consists of two steps; in the first step the GPI technique is applied to the Kalpana-IR data for rainfall estimation over the Indian land and oceanic region and in the second step an environmental moisture correction factor is applied to the GPI-based rainfall to estimate the final rainfall. Detailed validation with rain gauges and comparison with Tropical Rainfall Measuring Mission (TRMM) merged data product (3B42) are performed and it is found that the present technique is able to estimate the rainfall with better accuracy than the GPI technique over higher temporal and spatial domains for many operational applications in and around the Indian regions using Indian geostationary satellite data. Further comparison with the Doppler Weather Radar shows that the present technique is able to retrieve the rainfall with reasonably good accuracy.