SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

ISCCP cloud analysis algorithm intercomparison

The International Satellite Cloud Climatology Project (ISCCP) will provide a uniform global climatology of satellite-measured radiances and derive a climatology of cloud radiative properties from these radiances. For this purpose, a pilot study of cloud analysis algorithms was initiated to define a state-of-the-art algorithm for ISCCP. This study compared the results of applying the nine different algorithms to the same satellite radiance data. The comparison allowed for a sharper understanding of the process of detecting clouds and shows that all algorithms can be improved by better information about clear sky radiance values (essentially equivalent to surface property information) and by better understanding of cloud size distribution variations. The dependence of all methods on cloud size distribution led to selection of an advanced bispectral threshold technique for ISCCP because this method is currently better understood and more developed. Further research on cloud algorithms is clearly suggested by these results.     

Latitudinal Variation of the Underlying Heliospheric Magnetic Field Direction: Comparison of the Ulysses First and Second Orbits

We discuss the underlying direction of the heliospheric magnetic field measured by Ulysses in the latitude range 6° S-65° S by examining distributions of the magnetic field azimuthal angle with respect to the simple Parker spiral model. During the first Ulysses traversal of this latitude range in 1992–1994, while solar activity was declining, the shape of the distributions obtained at high latitudes in the fast solar wind differed from that at lower latitudes. In the present data set, obtained during rising solar activity, both field polarities are present at all latitudes and the peaks of the distributions agree with the predicted spiral direction to first approximation. However, compared to the first orbit, a significantly greater percentage of the observed field vectors have large deviations from the spiral direction. This revised version was published online in August 2006 with corrections to the Cover Date.     

A compact dopplergraph/magnetograph suitable for space-based measurements of solar oscillations and magnetic fields

A compact Dopplergraph/magnetograph placed in a continuous solar-viewing orbit will allow us to make major advancements in our understanding of solar internal structure and dynamics. An international program is currently being conducted at JPL and Mt. Wilson to develop such an instrument. By combining a unique magneto-optical resonance filter with CID and CCD cameras we have been able to obtain full- and partial-disk Dopplergrams and magnetograms. Time series of the velocity images are converted into k-ω power spectra which show clear- the solar nonradial p-mode oscillations. Magnetograms suitable for studying the long-term evolution of solar active regions have also been obtained with this instrument. A flight instrument based on this concept is being studied for possible inclusion in the SOHO mission.     

Ulysses charged particle measurements between 1 and 5 AU from the sun.

Proton fluxes obtained by two instruments carried on the ESA/NASA Ulysses spacecraft are reported for the period from launch in October 1990 till Jupiter encounter in February 1992. Proton energy ranges are 24-59, 71-99, 130-320, 320-2100 and > 2100 MeV. The Sun was very active during this period, the events of March 1991 being some of the largest of the solar cycle. The relationship between events on the Sun and the observed proton flux is discussed.     

Satellites for the study of ocean primary productivity

Estimates of global marine primary productivity are currently based upon the ¹⁴C method for determining rates of plankton photosynthesis and upon the relatively sparse data available using shipboard sampling techniques. With recent advances in remote sensing and in multiplatform (ship, aircraft, and satellite) sampling strategies, it is now possible to significantly lower the variance in estimates of phytoplankton abundance and of population growth rates.Multiplatform sampling strategies are essential to assess the mean and variance of phytoplankton biomass on a regional or on a global basis. The relative errors associated with shipboard and satellite estimates of phytoplankton biomass and primary productivity, as well as the increased statistical accuracy now possible from the utilization of contemporaneous data from both sampling platforms, are discussed. It is shown that one of the more exciting and potentially useful aspects of oceanographic research today is our new ability to view large areas of the ocean synoptically.     

The identification of mesospheric frontal gravity-wave events at a mid-latitude site

Mesospheric frontal-type gravity waves are an uncommon type of wave disturbance that occurs in the mesospheric OH, Na, O₂, and O(¹S) nightglow. They are understood to be the result of gravity waves exhibiting various degrees of non-linear behavior. Despite their similar appearance in all-sky images, careful analysis reveals that there are at least two distinct types of frontal wave disturbances, each with completely different consequences in terms of vertical momentum transport and deposition. Therefore, a correct identification is important in order to characterize their propagation modes. In this report we present the frontal gravity wave activity that occurred during a twelve-month period at Millstone Hill (42.6°N, 172.5°W), a mid-latitude site, to illustrate their range of behaviors.     

Remote sensing: A case for moving space data towards the public good

This paper discusses whether current international and national regulation of remote sensing activities achieves a true balance between proprietary interests of producers of remote sensing data and information and the needs of the community in accessing that data and information. By subjecting remote sensing data to general copyright restrictions that are often coupled with exclusive licences, irrespective of type or use of data and/or information, the development of important secondary information markets could be negatively hampered. In the long run, over-regulating access to space data may prove counter-productive in the information age. Using examples of different modes of information dissemination currently being practised, the paper highlights the balances to be drawn between legal issues of private ownership of data and information and public good interests. It concludes with suggestions for a more coherent regulatory approach.     

Magnetospheric electric field variations caused by storm-time shock fronts

On January 20, 2005 there was an X 7.1 solar flare at 0636 UT with an accompanied halo coronal mass ejection (CME). The resultant interplanetary shock impacted earth ∼36 h later. Near earth, the Advanced Composition Explorer (ACE) spacecraft observed two impulses with a staircase structure in density and pressure. The estimated earth-arrival times of these impulses were 1713 UT and 1845 UT on January 21, 2005. Three MINIature Spectrometer (MINIS) balloons were aloft on January 21st; one in the northern polar stratosphere and two in the southern polar stratosphere. MeV relativistic electron precipitation (REP) observed by all three balloons is coincident (<3 min) with the impulse arrivals and magnetospheric compression observed by both GOES 10 and 12. Balloon electric field data from the southern hemisphere show no signs of the impulse electric field directly reaching the ionosphere. Enhancement of the balloon-observed convection electric field by as much as 40 mV/m in less than 20 min during this time period is consistent with typical substorm growth. Precipitation-induced ionospheric conductivity enhancements are suggested to be (a) the result of both shock arrival and substorm activity and (b) the cause of rapid (<6 min) decreases in the observed electric field (by as much as 40 mV/m). There is poor agreement between peak cross polar cap potential in the northern hemisphere calculated from Super Dual Auroral Radar Network (SuperDARN) echoes and horizontal electric field at the MINIS balloon locations in the southern hemisphere. Possible reasons for this poor agreement include (a) a true lack of north–south conjugacy between measurement sites, (b) an invalid comparison between global (SuperDARN radar) and local (MINIS balloon) measurements and/or (c) radar absorption resulting from precipitation-induced D-region ionosphere density enhancements.