Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Transformation of a delta: Converting estuaries to lakes

In this century large-scale transformations have been carried out in the delta of the Rhine, Meuse and Scheldt in The Netherlands. The following principal consequences are considered: the loss of estuarine ecosystems, the change in the process of sedimentation and erosion, and the development of new aquatic and terrestrial ecosystems. The ecological consequences of the transformation of the Grevelingen estuary into a salt-water lake and some surprising developments are described briefly. The need for a limited restoration of the water exchange between the lake and the North Sea is indicated, as well as the major impacts of the exchange. The necessity for the maintenance or restoration of estuaries, the ecological development and the possibilities of the new lakes, and the management of these lakes by engineering structures are discussed. Careful land-use planning and ecosystem management, backed by research, are needed to make the fullest possible use of the potential offered by new lakes. It is also concluded that salt lakes have interesting perspectives.     

Attitude and Oribit Estimation Using Stars and Landmarks

An extended Kalman filter is used to process line-of-sight measurements to stars and known landmarks providing a statistical indication of performance in estimating spacecraft attitude, orbital ephemeris, and the bias drift of a set of three strapdown gyros. The landmark measurements were assumed to have been taken from the imagery of an Earth-observing multispectral scanner. It is shown that filtering of these noisy measurements results in highly accurate estimates of the above parameters. Results are given showing the sensitivity of performance to various system parameters such as star tracker accuracy, errors in the knowledge of landmark position, and number of stars and landmarks processed.     

The structural features of the germanium-silicon solid solution crystals obtained under microgravity

Structural features of germanium-silicon solid solution crystals have been investigated and silicon distribution in the crystals has been studied. All the crystals obtained under microgravity had, in spite of good external shape and facetting, a poorer internal structure than those obtained on Earth. The distribution of silicon have been shown to be non-uniform. High dislocation densities were observed.     

Minimum Number of Satellites for Three-Dimensional Continuous Worldwide Coverage

Global positioning by means of satellites requires simultaneous observation by at least four satellites. The problem is to determine the minimum number of satellites and the corresponding orbital geometry necessary to satisfy this requirement on a continuous basis. To model the problem, a fixed number of users are assumed uniformly distributed in a known manner over the surface of the earth, and the satellites are restricted to exist in either three or four orbital planes. However, the orbit radius and inclination angle are left as variables. Under these assumptions, and starting with a small number of satellites which will be increased afterwards, an algorithm is developed to determine the visibility of satellites at each surface location. In this way it is possible to specify the minimum number of satellites needed by any desired orbital geometry. It is found that the number of satellites required for three-dimensional continuous worldwide coverage decreases as the orbit radius is increased. There appears to be no general trend regarding the effect of the inclination angle on the minimum number of satellites.     

On the stereoselective aminoacylation of RNA

Aminoacylation of the “internal” 2′-hydroxyl groups of poly(A) with the imidazolide of racemic DL-leucine resulted in the formation of an enantiomeric excess of the D-ester, whereas aminoacylation with the imidazolide of racemic N-3,5-dinitrobenzoyl-DL-leucine gave an enantiomeric excess of the L-ester. Comparison of these results with those obtained earlier for alanine shows that the larger side chain tends to favor the formation of the D-ester. High resolution proton magnetic resonance spectroscopy was performed on the D- and L-alanyl “internal” 2′-esters of 3′,5′-ApA. The resonance of the side chain methyl group of the D-ester is upfield from that of the L-ester, and the separation of the peaks increases with a decrease in temperature.     

On the quasi-biennial oscillation in equatorial stratospheric temperatures and total ozone

The results of a cross-spectral analysis between monthly mean temperatures at 100 mb, 50 mb and 30 mb over the equator and the corresponding monthly mean BUV total ozone at different latitude zones are presented for the period 1970–1977. Significant squared coherences between total ozone and 50 mb equatorial temperatures at 26 months are only found between 5 degrees on each side of the equator, between 45 deg N and 55 deg N and at 45 deg S. At latitudes were the QBO in stratospheric temperature diminishes so does the QBO in total ozone (i.e. close to 35 deg N and 15 deg S). Over subtropical latitudes there is a tendency towards a more biennial oscillation in total ozone (not correlated with the equatorial QBO in stratospheric temperatures) and at 65 deg N and 65 deg S total ozone oscillates at periods greater than the equatorial QBO.     

Probability of Anomaly Expressions for Random Waveform Registration

Registration by integral-square error correlation of one-dimensional discrete-time waveforms which are treated as random processes with specified autocorrelation functions is considered. An important design parameter for this class of problems is the probability of anomaly (a false dip in the correlation function) because it gives an indication of system immunity to gross registration errors. Explicit expressions for this parameter are not possible, so bounds and appoximations must be derived. Two upper bounds and an approximation for the probability of anomaly are derived here. The use of these expressions is illustrated by an example. The relative utility of these performance indicators is shown for the example by comparison with actual values of the probability of anomaly obtained by computer simulation.     

Enhanced resolution in simple radars

Many simple radars use fact-risetime pulses, but wide bandwidth does not translate into corresponding high resolution since the spectrum is far from flat. A scheme for enhancing down- and cross-range resolution of multiple targets through a two-step partial equalization of the spectrum is illustrated by detailed computer simulation, with emphasis on the tradeoffs between resolution and signal-to-noise ratios. Three examples are treated: two equal scattering centers, two unequal scattering centers, and compound target     

Sensor compensation for vehicle magnetic signatures

Most land vehicles whose positions need to be tracked are made primary of steel. The problem considered is that of finding locations within or on land vehicles where magnetic sensors can be mounted to measure heading accurately and of finding compensation methods that minimize the effect of the vehicle magnetic signature. Results obtained with various types of vehicles in several world locations are given. Advantages and disadvantages of pendulous mounting are considered, and vehicle magnetic changes caused by driving in areas of extremely strong magnetic fields such as those generated by DC-powered trains are discussed