Characterizing parameters of Jatropha curcas cell cultures for microgravity studies

Jatropha (Jatropha curcas) is a tropical perennial species identified as a potential biofuel crop. The oil is of excellent quality and it has been successfully tested as biodiesel and in jet fuel mixes. However, studies on breeding and genetic improvement of jatropha are limited. Space offers a unique environment for experiments aiming at the assessment of mutations and differential gene expression of crops and in vitro cultures of plants are convenient for studies of genetic variation as affected by microgravity. However, before microgravity studies can be successfully performed, pre-flight experiments are necessary to characterize plant material and validate flight hardware environmental conditions. Such preliminary studies set the ground for subsequent spaceflight experiments. The objectives of this study were to compare the in vitro growth of cultures from three explant sources (cotyledon, leaf, and stem sections) of three jatropha accessions (Brazil, India, and Tanzania) outside and inside the petriGAP, a modified group activation pack (GAP) flight hardware to fit petri dishes. In vitro jatropha cell cultures were established in petri dishes containing a modified MS medium and maintained in a plant growth chamber at 25 ± 2 °C in the dark. Parameters evaluated were surface area of the explant tissue (A), fresh weight (FW), and dry weight (DW) for a period of 12 weeks. Growth was observed for cultures from all accessions at week 12, including subsequent plantlet regeneration. For all accessions differences in A, FW and DW were observed for inside vs. outside the PetriGAPs. Growth parameters were affected by accession (genotype), explant type, and environment. The type of explant influenced the type of cell growth and subsequent plantlet regeneration capacity. However, overall cell growth showed no abnormalities. The present study demonstrated that jatropha in vitro cell cultures are suitable for growth inside PetriGAPs for a period of 12 weeks. The parameters evaluated in this study provide the basic ground work and pre-flight assessment needed to justify a model for microgravity studies with jatropha in vitro cell cultures. Future studies should focus on results of experiments performed with jatropha in vitro cultures in microgravity.     

Comparison of OClO nadir measurements from SCIAMACHY and GOME

The scanning imaging absorption spectrometer for atmospheric chartography was launched successfully onboard ENVISAT on March 1, 2002. It observes the solar radiation transmitted and backscattered from the atmosphere and reflected from the ground in nadir, limb and occultation viewing modes. Chlorine dioxide (OClO), an important indicator for stratospheric chlorine activation, can be measured in the UV spectral range by differential optical absorption spectroscopy (DOAS).

First results of the DOAS retrieval of OClO slant column densities from the SCIAMACHY nadir measurements are presented and compared to measurements of the global ozone monitoring experiment (GOME), which has successfully measured OClO since 1995. While SCIAMACHY operates in the same orbit, it measures ≈30 min earlier than GOME and has an increased spatial resolution (30 × 60 km² compared to 40 × 320 km² for GOME).     

Gravity sensing in moss protonemata.

Moss protonemata are a valuable system for studying gravitropism because both sensing and upward curvature (oriented tip growth) take place in the same cell. We review existing evidence, especially for Ceratodon purpureus, that addresses whether the mass that functions in sensing is that of amyloplasts that sediment. Recent experiments show that gravitropism can take place in media that are denser than the apical cell. This indicates that gravity sensing relies on an intracellular mass rather than that of the entire cell and provides further support for the starch-statolith hypothesis of sensing. Possible mechanisms for how amyloplast mass functions in sensing and transduction are discussed.     

Experimental investigations on hull-fin interferences of the LOTTE airship

In the present paper experimental aerodynamic investigations on an airship configuration at angle of attack with special emphasis on the hull-fin region are reported. In particular, visualizations of flow phenomena on both hull and fins are studied. Quantitative measurements of the integral force and moment characteristics as well as local pressure coefficients serve to establish a data pool for code validation.     

Comparison of hindlimb unloading and partial weight suspension models for spaceflight-type condition induced effects on white blood cells

Animal models are frequently used to assist in the determination of the long- and short-term effects of space flight. The space environment, including microgravity, can impact many physiological and immunological system parameters. It has been found that ground based models of microgravity produce changes in white blood cell counts, which negatively affects immunologic function. As part of the Center of Acute Radiation Research (CARR), we compared the acute effects on white blood cell parameters induced by the more traditionally used animal model of hindlimb unloading (HU) with a recently developed reduced weightbearing analog known as partial weight suspension (PWS). Female ICR mice were either hindlimb unloaded or placed in the PWS system at 16% quadrupedal weightbearing for 4 h, 1, 2, 7 or 10 days, at which point complete blood counts were obtained. Control animals (jacketed and non-jacketed) were exposed to identical conditions without reduced weightbearing. Results indicate that significant changes in total white blood cell (WBC), neutrophil, lymphocyte, monocyte and eosinophil counts were observed within the first 2 days of exposure to each system. These differences in blood cell counts normalized by day 7 in both systems. The results of these studies indicate that there are some statistically significant changes observed in the blood cell counts for animals exposed to both the PWS and HU simulated microgravity systems.     

Application of existing launch and flight system hardware for early SEI mission

A major paradigm shift in space exploration is at hand. While great discipline must be exercised, we can reinstitute the exploration of the Moon and Mars through use of current systems and technology. These early initiatives will require coupling available or growth boosters with evolving upper stages and spacecraft that employ new emerging technologies and subsystems to characterize the resources, topography, gravity and sites for future manned expeditions. We have the opportunity to perform first-rate scientific research while also serving the needs of the unfolding Space Exploration Initiative. These early programs can be pursued within limited budgets if management techniques, best described as “fast track”, are applied. Examples of such efforts that met all federal regulations, yet achieved significant returns in 1- to 2-year spans, are given.     

The german microgravity program: Objectives and present status

Within the space program of the Federal Republic of Germany the microgravity program in connection with the utilization of SPACELAB constitutes a central task which determines the long-term program concepts and also their relation to German participation in future ESA programs.The scientific preparatory programs under way for some years now have made further progress. Extensive flight experience and valuable scientific results were obtained on the basis of successful rocket pre-programs. The present paper describes the process in which scientific and organisational priorities are being defined for the planning and execution of the experimental programs.In order to obtain a sufficient number of flight opportunities, payloads for SPACE SHUTTLE missions, in particular under the NASA GAS Program, as well as experimental equipment such as the materials laboratory (MSDR) for FSLP are being developed. The German program focuses on preparing a German SPACELAB mission D1 planned for 1985, which is intended to verify the applicability and efficiency of manned research laboratories for industry and the scientific community. A second emphasis is on preparing the use of SHUTTLE-supported re-usable space platforms.     

A slotted waveguide array antenna from carbon fibre reinforced plastics for the European space SAR

This paper deals with the design of a Synthetic Aperture Radar (SAR)-antenna for the proposed European Remote Sensing Satellite. Electrical performance requirements and geometric constraints impose stringent conditions upon structural configuration and mechanical tolerances. A slotted waveguide array antenna is presented in a deployable configuration. It is composed of 5 radiating panels, and a foldable truss structure. Carbon fibre reinforced plastic material is used for structural elements and waveguides.     

A wave model for dwarf novae

The rapid coherent oscillation during a dwarf nova outburst is attributed to an accretion-driven wave going around the white dwarf component of the binary system. The increase and decrease in the period of this oscillation is due to the change in the velocity of the wave as it is first being driven and then damped. Qualitatively, a large number of observations can be explained with such a model. The beginnings of a mathematical representation of this model are developed.     

Experiments on velocity augmentation of spherical flames by grids

Using spherical wire mesh screens with a wire diameter d and a mesh size l, an experimental study was carried out involving homogeneous mixtures of propane/air, ethylene/air, acetylene/air and with oxygen enriched air. Streak photographs of the processes were taken, yielding information on the flame propagation speeds inside and outside the screen. The ratio of the two speeds immediately before and after crossing the screen, α, can be interpreted as practically equivalent (for the same “heat release”) to the ratio of flame velocities relative to the unburned gas, i.e. Values of (α − 1) are correlated with the Reynolds number based on the wire thickness d, density and viscosity of the unburned gas and flame propagation speed inside the wire mesh. The experiments were repeated with two and three spheres of different diameters, e.g. inner screen 9 cm in diameter and outer screen 18 cm in diameter or three screens, 9, 13.5 and 18 cm, respectively. The second screen usually added a factor of 2 on the value of α. The third screen produced very little additional effect. The highest value of α we ever obtained for mixtures with air was about 12 (for acetylene/air). With oxygen enriched air the situation is quite different. If the flame velocity of the laminar flame relative to the unburned gas exceeds 2 m/sec, the value of α may increase up to a level corresponding to detonation.