Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

Closed Artificial ecosystems as a means of ecosystem studies for Earth and space needs.

Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together "holistic" and "merological" approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of "ecosystems health" concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. Grant numbers: N 99-04-96017, N25.     

Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

1.

Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.     

Mean energy of response to galactic cosmic ray spectrum: IMP 8 and Climax neutron monitor

Solar modulations of galactic cosmic ray (GCR) intensity contain a wealth of information about their transport in the heliosphere. To extract this information from the data one studies the dependence of the observed modulations on the mean energy of response of detectors providing data for the analyses. There is a great deal of confusion about the detector energy response to GCR spectrum in the literature. We present a preliminary report on the computations of the mean energy of response for the Climax neutron monitor (CL/NM) and IMP 8 cosmic ray nuclear composition instrument to GCR protons for 1973–1998, covering the solar cycles 21 and 22. We find that for penetrating proton channel on IMP 8 the mean energy changes by a factor of over two whereas for the neutron monitor the change is only 21%. However, the corresponding change for the computed modulation function is a factor of about 3.5.     

Aerosols and clouds in the upper troposphere–lower stratosphere region detected by GOMOS and ACE: Intercomparison and analysis of the years 2004 and 2005

Satellite-based limb occultation measurements are well suited for the detection and mapping of polar stratospheric clouds (PSCs) and cirrus clouds. PSCs are of fundamental importance for the formation of the Antarctic ozone hole that occurs every year since the early 1980s in Southern Hemisphere spring. Despite progress in the observation, modeling and understanding of PSCs in recent years, there are still important questions which remain to be resolved, e.g. PSC microphysics, composition, formation mechanisms and long-term changes in occurrence. In addition, it has recently become clear that cirrus clouds significantly affect the global energy balance and climate, due to their influence on atmospheric thermal structure.     

Remote upgrading of a space-borne instrument

The main purposes of experiment “Obstanovka” (“Environment” in Russian) consisting of several instruments are to measure a set of electromagnetic and plasma phenomena characterizing the space weather conditions, and to evaluate how such a big and highly energy consuming body as the International Space Station disturbs the surrounding plasma, and how the station itself is charged due to the operation of so many instruments, solar batteries, life supporting devices, etc. Two identical Langmuir electrostatic probes are included in the experiment “Obstanovka”. In this paper the Langmuir probes for “Obstanovka” experiment are described, including the choice of geometry (spherical or cylindrical), a more reliable method for the sweep voltage generation, an adaptive algorithm for the probe’s operation. Special attention is paid to the possibility for remote upgrading of the instrument from the ground using the standard communication channels.     

Interstellar planetary protection

In the coming decades the detection of Earth-like extrasolar planets, either apparently lifeless or exhibiting spectral signatures of life, will encourage design studies for craft to visit them. These missions will require the elaboration of an interstellar planetary protection protocol. Given a specific dose required to sterilize microorganisms on a spacecraft, a critical mean velocity can be determined below which a craft becomes self-sterilizing. This velocity is calculated to be below velocities previously projected for interstellar missions, suggesting that an active sterilization protocol prior to launch might be required. Given uncertainties in the surface conditions of a destination extrasolar planet, particularly at microscopic scales, the potential for unknown biochemistries and biologies elsewhere, or the possible inoculation of a lifeless planet that is habitable, then both lander and orbiter interstellar missions should be completely free of all viable organisms, necessitating a planetary protection approach applied to orbiters and landers bound for star systems with unknown local conditions for habitability. I discuss the case of existing craft on interstellar trajectories – Pioneer 10, 11 and Voyager 1 and 2.     

In situ analysis of Europa ices by short-range melting probes

A key aspect for understanding the astrobiological potential of planets and moons in the Solar system is the analysis of material embedded in or underneath icy layers on the surface. In particular in case of the icy crust of Jupiters moon Europa such investigation would be of greatest interest. For a Europa lander to be launched in the 2020–2030 timeframe, we propose to use a simplified instrumented melting probe which is able to access and sample depths of a few meters without the necessity of heavy and complicated drilling equipment.