Identification of specific gravity sensitive signal transduction pathways in human A431 carcinoma cells.

Epidermal growth factor (EGF) activates a well characterized signal transduction cascade in human A431 epidermoid carcinoma cells. The influence of gravity on EGF-induced EGF-receptor clustering and early gene expression as well as on actin polymerization and actin organization have been investigated. Different signalling pathways induced by the agents TPA, forskolin and A23187 that activate gene expression were tested for sensitivity to gravity. EGF-induced c-fos and c-jun expression were decreased in microgravity. However, constitutive beta-2 microglobulin expression remained unaltered. Under simulated weightlessness conditions EGF- and TPA-induced c-fos expression was decreased, while forskolin- and A23187-induced c-fos expression was independent of the gravity conditions. These results suggest that gravity affects specific signalling pathways. Preliminary results indicate the EGF-induced EGF-receptor clustering remained unaltered irrespective of the gravity conditions. Furthermore, the relative filamentous actin content of steady state A431 cells was enhanced under microgravity conditions and actin filament organization was altered. Under simulated weightlessness actin filament organization in steady state cells as well as in EGF-treated cells was altered as compared to the 1 G reference experiment. Interestingly the microtubule and keratin organization in untreated cells showed no difference with the normal gravity samples. This indicates that gravity may affect specific components of the signal transduction circuitry.     

Marangoni convection in a liquid layer under the simultaneous action of a transverse magnetic field and rotation

Asymptotic stability characteristics with respect to the onset of Marangoni convection in a liquid layer under the simultaneous action of a large transverse magnetic field and a large rate of rotation are investigated. In certain parameter ranges there is a decrease in critical Marangoni number for increasing magnetic field and rotation depending on the coupling between interfacial perturbations and rotation.     

IR-spectroscopy of ionosphere from stratospheric balloons

The radiation spectra of the ionosphere in the 4–5 μm region have been determined from stratospheric balloons by means of the specially elaborated method of the registration of angular and spectral distribution of the radiation. The radiation bands of 4.4 μm and 4.8 μm which have 0.1–0.2 erg cm^?2 sr^?1s^?1. brightness obtained in two flights, are identified with the vibration-rotation transitions of the ion NO⁺ (0 - 1) and the molecules N¹⁴N¹⁵ (0 - 1) and CO (0 - 1) and (3 - 2) from altitudes between 120 and 500 km.     

Vertical profiles of halocarbons in the stratosphere

Stratospheric air samples collected between 10 and 35 km altitude my means of a cryogenic sampler were analyzed by gaschromatography. Thus vertical profiles of source gases for halogen radicals were derived, such as CCl₄, CCl₃F, CCl₂F₂, CClF₃, CF₄, C₂F₃Cl₃, C₂F₄Cl₂, C₂F₅Cl, C₂F₆, CH₃Cl, CH₃CCl₃, CHF₂Cl, CH₃Br, CBrF₃, and CBrCl₂F. Systematic discrepancies between measured and modelled halocarbon profiles point to deficiencies of present one- and two-dimensional models. Measurements of fully halogenated hydrocarbons provide a tool for systematically studying these deficiencies and thus improving the models.     

ELF radio signals produced in the auroral ionosphere by non-linear demodulation of signals from high-power amplitude-modulated transmitters

ELF and VLF radio signals were recorded in the afternoon to early morning (local time) between 24 March and 4 April 1979, in Northern Scandinavia. Apart from signals of natural origin, timing signals, i.e. six pips of equal duration of 105 ± 8 ms, at 1 kHz ± 0.5 Hz, were observed on the hour UT. Such signals only occur on days of relatively high geomagnetic activity during enhanced auroral electrojet activity. They are believed to be generated by non-linear demodulation (self-detection) of signals from two or more amplitude modulated transmitters in the USSR, operating at 173, 200, 236, 263 and 657 kHz. The simplest explanation for the observations is provided by the three transmitters operating at 173 kHz.     

Gravitational influence on binary alloy melt equilibria

Micro- and macrosegregation, the nonuniform redistribution of solute during solidification, are common to both casting and welding, processes of fundamental importance in materials engineering. In multicomponent crystal growth where solid/liquid density differences are appreciable gravity-induced separation can lead to significant spatial variations in resulting ingot composition. In fact, this phenomenon is also operative in liquid/liquid systems such as monotectic alloys exhibiting a liquid miscibility gap, where buoyancy-driven flows can result in a sometimes unwanted separation of phases upon cooling through the miscibility dome.     

Modeling Random Gyro Drift Rate by Data Dependent Systems

A new modeling procedure is presented in which a mathematical model for random gyro drift rate is obtained directly from the original observations without requiring any theoretical conjectures or preprocessing of the data. The adequate model so obtained confirms the presence of a random walk component causing the "steady state" drift and a stochastic component of the nature of correlated noise. It also provides a quantitative measure of the relative contribution of each component, useful in determining the quality of the gyro in guidance over extended periods.     

Application of remote sensing techniques in mineral resources survey — a case study pertaining to Singhbhum Shear Zone,Bihar, India

Geological analysis and evaluation of digitally processed and enhanced LANDSAT MSS data products for three test areas of Singhbhum Shear Zone, India has led to certain significant results regarding optimisation and utility of such techniques for geological studies and obtaining additional/new information regarding lithology and structure. Three band-to-band ratio images are found to give best results. Lithologic formations could be subdivided into a number of units. The Westward and southeastward extension of the shear zone could be delineated thereby indicating scope of mineralisation in these parts.     

Skylab experiment results: hematology studies

These studies were designed and coordinated to evaluate specific aspects of man's immunologic and hematologic systems which might be altered by or respond to the space flight environment. The biochemical functions investigated included cytogenetic damage to blood cells, immune resistance to disease, regulation of plasma and red cell volumes, metabolic processes of the red blood cell, and physical chemical aspects of red blood cell functions. Only minor changes were observed in the functional capacity of erythrocytes as determined by measuring the concentrations of selected intracellular enzymes and metabolites. Tests of red cell osmotic regulation indicated some elevation in the activity of the metabolic dependent Na-K pump, with no significant alterations in the cellular Na and K concentrations or osmotic fragility. A transient shift in red cell specific-gravity profile was observed on recovery, possibly related to changes in cellular water content. Measurements of hemoconcentration (hematocrit, hemoglobin concentration, red cell count) indicated significant fluctuations postflight, reflecting observed changes in red cell mass and plasma volume. There was no apparent reticulocytosis during the 18 days following the first manned Skylab mission in spite of a significant loss in red cell mass. However, the reticulocyte count and index did increase significantly 5 to 7 days after completion of the second, longer duration, flight. There were no significant changes in either the while blood cell count or differential. However, the capacity of lymphocytes to respond to an in vitro mitogenic challenge was repressed postflight, and appeared to be related to mission duration. The cause of this repression is unknown at this time. Only minor differences were observed in plasma protein patterns. In the second mission there were changes in the proteins involved in the coagulation process which suggested a hypercoagulative condition.