Determination of lethality rate constants and D-values for heat-resistant Bacillus spores ATCC 29669 exposed to dry heat from 125°C to 200°C

Exposing flight hardware to dry heat is a NASA-approved sterilization method for reducing microbial bioburden on spacecraft. The existing NASA specification only allows heating the flight hardware between 104°C and 125°C to reduce the number of viable microbes and bacterial spores. Also, the NASA specifications only allow a four log reduction by dry heat microbial reduction because very heat-resistant spores are presumed to exist in a diverse population (0.1%). The goal of this research was to obtain data at higher temperatures than 125°C for one of the most heat-resistant microorganisms discovered in a spacecraft assembly area. These data support expanding the NASA specifications to temperatures higher than 125°C and relaxing the four log reduction specification. Small stainless steel vessels with spores of the Bacillus strain ATCC 29669 were exposed to constant temperatures between 125°C and 200°C under both dry and ambient room humidity for set time durations. After exposures, the thermal spore exposure vessels were cooled and the remaining spores recovered and plated out. Survivor ratios, lethality rate constants, and D-values were determined at each temperature. The D-values for the spores exposed under dry humidity conditions were always found to be shorter than those under ambient humidity. The temperature dependence of the lethality rate constants was obtained by assuming that they obeyed Arrhenius behavior. The results are compared to those of B. atrophaeus ATCC 9372. In all cases, the D-values of ATCC 29669 are between 20 and 50 times longer than those of B. atrophaeus ATCC 9372.     

Photochemical synthesis of citric acid cycle intermediates based on titanium dioxide

The emergence of the citric acid cycle is one of the most remarkable occurrences with regard to understanding the origin and evolution of metabolic pathways. Although the chemical steps of the cycle are preserved intact throughout nature, diverse organisms make wide use of its chemistry, and in some cases organisms use only a selected portion of the cycle. However, the origins of this cycle would have arisen in the more primitive anaerobic organism or even back in the proto-metabolism, which likely arose spontaneously under favorable prebiotic chemical conditions. In this context, we report that UV irradiation of formamide in the presence of titanium dioxide afforded 6 of the 11 carboxylic acid intermediates of the reductive version of the citric acid cycle. Since this cycle is the central metabolic pathway of contemporary biology, this report highlights the role of photochemical processes in the origin of the metabolic apparatus.     

The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payload

We report the first telemetered spaceflight science results from the orbiting Space Environment Survivability of Living Organisms (SESLO) experiment, executed by one of the two 10?cm cube-format payloads aboard the 5.5?kg Organism/Organic Exposure to Orbital Stresses (O/OREOS) free-flying nanosatellite. The O/OREOS spacecraft was launched successfully to a 72° inclination, 650?km Earth orbit on 19 November 2010. This satellite provides access to the radiation environment of space in relatively weak regions of Earth's protective magnetosphere as it passes close to the north and south magnetic poles; the total dose rate is about 15 times that in the orbit of the International Space Station. The SESLO experiment measures the long-term survival, germination, and growth responses, including metabolic activity, of Bacillus subtilis spores exposed to the microgravity, ionizing radiation, and heavy-ion bombardment of its high-inclination orbit. Six microwells containing wild-type (168) and six more containing radiation-sensitive mutant (WN1087) strains of dried B. subtilis spores were rehydrated with nutrient medium after 14 days in space to allow the spores to germinate and grow. Similarly, the same distribution of organisms in a different set of microwells was rehydrated with nutrient medium after 97 days in space. The nutrient medium included the redox dye Alamar blue, which changes color in response to cellular metabolic activity. Three-color transmitted intensity measurements of all microwells were telemetered to Earth within days of each of the 48?h growth experiments. We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments.     

积极主动的发动机维修

运营商越来越多地发现发动机预防性维修的长期价值. 要使航空公司能够对发动机维修变得更为积极主动,说起来容易做起来难,即使航空运输回暖和航空公司收入增加的情况下也是这样.     

The Effect of Hard Limiting in the Presence of Large Out-of-Band Interfering Signals

The effect of hard limiting on the phase of a test signal was investigated when large out-of-band interfering signals are present at the input to the limiter. First a single and then two discrete interfering signals were considered. The interfering signals and the test signal are restricted to narrow but separated bands. The test signal is recovered after limiting by narrowband filtering. The analysis of the single interfering signal is essentially the same as that performed by Cahn.1 In this correspondence experimental evidence is presented to verify the analysis and, in addition, the case of two interfering signals is considered.     

Ocean Engineering in the U. S. Navy

This paper is the complete text of an address given at the IEEE meeting in New York on March 23, 1966, in ?Undersea Technology Session,? covering the U. S. Navy's furtherance of the development of Ocean Engineering and some plans for the future. The items covered include the deep submergence systems project, SEALAB III, salvage work packages circa 1967, and additions to the Marine Engineering Laboratory.     

The new millennium program: Fast-track procurements

The National Aeronautics and Space Administration's (NASA's) New Millennium Program (NMP) has embarked on a technology flight-validation demonstration program to enable the kinds of missions that NASA envisions for the 21st century. Embedded in this program is the concept of rapid mission development supported by a fast-track procurement process. This process begins with the decision to initiate a procurement very early in the program along with the formation of a technical acquisition team. A close working relationship among the team members is essential to avoiding delays and developing a clear acquisition plan. The request for proposal (RFP) that is subsequently issued seeks a company with proven capabilities, so that the time allotted for responses from proposers and the length of proposals they submit can be shortened. The fast-track procurement process has been demonstrated during selection of NMP's industrial partners and has been proven to work.     

Application of time-variable process noise in terrestrial reference frames determined from VLBI data

In recent years, Kalman filtering has emerged as a suitable technique to determine terrestrial reference frames (TRFs), a prime example being JTRF2014. The time series approach allows variations of station coordinates that are neither reduced by observational corrections nor considered in the functional model to be taken into account. These variations are primarily due to non-tidal geophysical loading effects that are not reduced according to the current IERS Conventions (2010). It is standard practice that the process noise models applied in Kalman filter TRF solutions are derived from time series of loading displacements and account for station dependent differences. So far, it has been assumed that the parameters of these process noise models are constant over time. However, due to the presence of seasonal and irregular variations, this assumption does not truly reflect reality. In this study, we derive a station coordinate process noise model allowing for such temporal variations. This process noise model and one that is a parameterized version of the former are applied in the computation of TRF solutions based on very long baseline interferometry data. In comparison with a solution based on a constant process noise model, we find that the station coordinates are affected at the millimeter level.     

The Morphologies and Kinematics of Supernova Remnants

We review the major advances in understanding the morphologies and kinematics of supernova remnants (SNRs). Simulations of SN explosions have improved dramatically over the last few years, and SNRs can be used to test models through comparison of predictions with SNRs’ observed large-scale compositional and morphological properties as well as the three-dimensional kinematics of ejecta material. In particular, Cassiopeia A—the youngest known core-collapse SNR in the Milky Way—offers an up-close view of the complexity of these explosive events that cannot be resolved in distant, extragalactic sources. We summarize the progress in tying SNRs to their progenitors’ explosions through imaging and spectroscopic observations, and we discuss exciting future prospects for SNR studies, such as X-ray microcalorimeters.     

Editorial: Topical Collection on Multi-Point Measurements of the Thermosphere with the QB50 Mission

Space Science Reviews - Part of the InSight mission, the SEIS instrument (Seismic Experiment for Interior Structures), is planned to arrive on Mars in November 2018. In order to prepare its future...